Extraction and structural characteristics of extracellular polymeric substances (EPS), pellets in autotrophic nitrifying biofilm and activated sludge

Liang, Zhiwei; Li, Wenhong; Yang, Shiling; Du, Ping

doi:10.1016/j.chemosphere.2010.03.043

Cited by 254 publications

(71 citation statements)

References 29 publications

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“…Compared with the SEPS and LB-EPS, the Raman spectra of TB-EPS had changed significantly. The infrared spectra also demonstrated the disparity between TB-EPS and other EPS fractions (Liang et al, 2010;Chen et al, 2013a). The TB-EPS from the S-sludge and biofilm have also similar qualitative Raman spectra, which Raman peaks were primary assigned the functional groups of proteins and polysaccharides (Fig.…”

Section: Eps Characterization By Ramanmentioning

confidence: 89%

“…The SEPS and LB-EPS of the biofilm and S-sludge samples were extracted using the modified method described by Liang et al (2010). Approximately 20 mL of fresh samples were placed in 50 mL centrifuge tubes, mixed with deionized water to form a 45 mL suspension, and then treated by ultrasound at 20 kHz and 40 W for 30 s. The suspensions were then centrifuged at 2000g for 15 min at 4°C.…”

Section: Eps Extractionsmentioning

confidence: 99%

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Composition of EPS fractions from suspended sludge and biofilm and their roles in microbial cell aggregation

et al. 2014

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Section: Eps Characterization By Ramanmentioning

confidence: 89%

Section: Eps Extractionsmentioning

confidence: 99%

Composition of EPS fractions from suspended sludge and biofilm and their roles in microbial cell aggregation

et al. 2014

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“…For this reason it is possible that some intracellular material is extracted together with the EPS. Although cell lysis can be caused by physical and chemical extraction techniques (sonication 31,32 , NaOH 31,32 , EDTA 11,32 , CER 32 , heat 32 and high shear rates by mixing 19 ), the presence of intracellular material in recovered EPS still needs to be verified. The ionic gel-forming property of the extracted EPS is the main focus of this research, whether the recovered EPS contains intracellular material was not analyzed.…”

Section: Remarks For the Protocol Sectionmentioning

confidence: 99%

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge

Felz

Al-Zuhairy

Aarstad

et al. 2016

JoVE

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To evaluate and develop methodologies for the extraction of gel-forming extracellular polymeric substances (EPS), EPS from aerobic granular sludge (AGS) was extracted using six different methods (centrifugation, sonication, ethylenediaminetetraacetic acid (EDTA), formamide with sodium hydroxide (NaOH), formaldehyde with NaOH and sodium carbonate (Na 2 CO 3 ) with heat and constant mixing). AGS was collected from a pilot wastewater treatment reactor. The ionic gel-forming property of the extracted EPS of the six different extraction methods was tested with calcium ions (Ca 2+ ). From the six extraction methods used, only the Na 2 CO 3 extraction could solubilize the hydrogel matrix of AGS. The alginatelike extracellular polymers (ALE) recovered with this method formed ionic gel beads with Ca 2+. The Ca 2+ -ALE beads were stable in EDTA, formamide with NaOH and formaldehyde with NaOH, indicating that ALE are one part of the structural polymers in EPS. It is recommended to use an extraction method that combines physical and chemical treatment to solubilize AGS and extract structural EPS.

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“…The samples after nitrite pretreatment were extracted using the modified method described by Liang et al and to obtain extracellular polymeric substances (EPS) (Liang et al 2010).…”

Section: Eps Extractionmentioning

confidence: 99%

Hydrolysis and volatile fatty acids accumulation of waste activated sludge enhanced by the combined use of nitrite and alkaline pH

Huang

Liu

Sun

et al. 2015

Environ Sci Pollut Res

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Volatile fatty acids (VFAs) production from anaerobic digestion of waste activated sludge (WAS) is often limited by the slow hydrolysis and/or poor substrate availability. Increased attention has been given to enhance the hydrolysis and acidification of WAS recently. This study presented an efficient and green strategy based on the combined use of nitrite pretreatment and alkaline pH to stimulate hydrolysis and VFA accumulation from WAS. Results showed that both proteins and polysaccharides increased in the presence of nitrite, indicating the enhancement of sludge solubilization and hydrolysis processes. Mechanism investigations showed that nitrite pretreatment could disintegrate the sludge particle and disperse extracellular polymeric substances (EPS). Then, anaerobic digestion tests demonstrated VFA production increased with nitrite treatment. The maximal VFA accumulation was achieved with 0.1 g N/L nitrite dosage and pH 10.0 at a sludge retention time (SRT) of 7 days, which was much higher VFA production in comparison with the blank, sole nitrite pretreatment, or sole pH 10. The potential analysis suggested that the combined nitrite pretreatment and alkaline pH is capable of enhancing WAS digestion with a great benefit for biological nutrient removal (BNR).

show abstract

Extraction and structural characteristics of extracellular polymeric substances (EPS), pellets in autotrophic nitrifying biofilm and activated sludge

Cited by 254 publications

References 29 publications

Composition of EPS fractions from suspended sludge and biofilm and their roles in microbial cell aggregation

Composition of EPS fractions from suspended sludge and biofilm and their roles in microbial cell aggregation

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge

Hydrolysis and volatile fatty acids accumulation of waste activated sludge enhanced by the combined use of nitrite and alkaline pH

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