Extraction and structural characterization of collagen from fishbone by high intensity pulsed electric fields

Abstract

Collagen was extracted from fishbone by high intensity pulsed electric fields (PEF) and its structure was characterized. A maximum collagen yield of 16.13 mg/mL was achieved under the following conditions: pepsin dosage of 1%, material‐liquid ratio of 1:10, electric field strength of 20 kV/cm, and pulse number of 8. The change of each secondary structure component content was highly correlated with the PEF operating parameters (R2 > .900). The tertiary structure of collagen was changed by PEF. The surface hydrophobicity increased first and then decreased with the PEF operating parameters. Scanning electron microscopy results showed the tissues changes of sample particles from dispersed and disorganized to uniform by PEF, but the high electric field strength (>30 kV/cm) and pulse number (>8) causes samples over agglomerated, which were dispersed disorderly. This article has proposed a new approach for comprehensive utilization of fishbone, which has important research significance for the promotion of PEF. Practical Applications Collagen was extracted from fishbone by high intensity pulsed electric fields. The prepared fishbone collagen can be widely used in foods, cosmetics, biology, fertilizers, medical materials, and other fields. This article provides a new technique for comprehensive utilization of fishbone. The structure of extracted collagen was characterized. This opens up a new dimension in the study of the impact of PEF technology on protein structure.

Fish Collagen: Extraction, Characterization, and Applications for Biomaterials Engineering

“…The same group [ 58 ] in a recent study achieved a maximum collagen yield of 16.13 mg/mL from fish bones using pepsin 1%, with a PEF strength of 20 kV/cm. Desalting is also suggested as an essential process in bone collagen extraction due to its high hydroxyapatite and calcium content, which are removed by EDTA or HCl during the pretreatment; however, using HCl can degrade the collagen [ 54 ].…”

Subcritical Water Extraction and Hydrolysis of Cod (Gadus morhua) Frames to Produce Bioactive Protein Extracts

“…Jafarpour et al [8] have recently investigated the valorization of cod frames by means of enzymatic extraction and hydrolysis. Other authors have proposed the intensification of the process through extrusion methods [7], ultrasound assisted extraction (UAE) [9], or the application of pulsed electric fields [10].…”

Valorisation of food processing wastes using PEF and its economic advances – recent update

“…The collagen extracted from fish by‐products can be used as functional ingredients in food, chemical, medical, cosmetic and fertiliser fields. They have a significant role in maintaining physiological activities of the human body such as anti‐ageing, anti‐hypertension, improving bone density, platelet aggregation, skin metabolism, and bone formation (He et al ., 2019). Also, the texturising, gelling, and thickening properties of exhibited by the extracted collagen from fish bone can be utilised in food and beverage processing industries (Coppola et al ., 2020).…”