Extraction and subsequent determination of dialkyl phthalates in the soil using gas chromatography combined with tandem mass spectrometry

Пирогов, А. В.; Tolmacheva, N. G.; Шпигун, О. А.

doi:10.3103/s0027131414040087

Cited by 3 publications

(5 citation statements)

References 6 publications

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“…Phthalates were extracted with n-hexane (10 mL sample + 1 mL n-hexane). The determination was carried out by gas chromatography-mass spectrometry in the mode of registration of isolated ions [51,52]. Briefly, the column HP-5MS (30 m × 250 microns × 0.25 microns) ("Agilent Technologies", Santa Clara, CA, USA) was used, and the mobile phase was helium and the eluent flow rate was 1 mL/min.…”

Section: Determination Of Dbp In Water Samples By Gc-msmentioning

confidence: 99%

“…Temperature gradient was from 40 to 130 • C at a speed of 50 • C/min, from 130 to 250 • C at a speed of 5 • C/min, and from 250 to 300 • C at a speed of 10 • C/min. The source temperature was 230 • C. Mass spectrometric detection was performed on the following ion: m/z 149 [51,52]. An auxiliary m/z 223 peak was used to confirm the detection of DBP [53].…”

Section: Determination Of Dbp In Water Samples By Gc-msmentioning

confidence: 99%

“…To determine the level of phthalate contamination of Lake Onega, 16 water samples were collected. These samples were initially tested by GC-MS [50,52]. After analysis, we selected two samples that did not contain phthalates (#1 and #2).…”

Section: Determination Dbp In Real Samplesmentioning

confidence: 99%

“…A notable feature observed in the mass spectra of phthalates is the presence of a common fragment ion of protonated phthalate anhydride with a mass-to-charge ratio of m/z 149. This mass peak is characteristic of most phthalates with longer alkyl substituents and was used to identify DBP [52]. An auxiliary m/z 223 peak was used to confirm the detection of DBP (Figure S6) [54].…”

Section: Determination Dbp In Real Samplesmentioning

confidence: 99%

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Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay

Mukhametova,

Karimova,

Zharikova

et al. 2023

Biosensors

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Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to give them flexibility, strength and extensibility. However, due to its negative impact on human health, in particular reproductive functions and fetal development, the content of DBP must be controlled in food and the environment. The present study aims to develop a sensitive, fast and simple fluorescence polarization immunoassay (FPIA) using monoclonal antibodies derived against DBP (MAb-DBP) for its detection in open waters. New conjugates of DBP with various fluorescein derivatives were obtained and characterized: 5-aminomethylfluorescein (AMF) and dichlorotriazinylaminofluorescein (DTAF). The advantages of using the DBP-AMF conjugate in the FPIA method are shown, the kinetics of binding of this chemical with antibodies are studied, the analysis is optimized, and the concentration of monoclonal antibodies is selected for sensitivity analysis—16 nM. The calibration dependence of the fluorescence polarization signal for the detection of DBP was obtained. The observed IC50 (DBP concentration at which a 50% decrease in the fluorescence polarization signal occurs, 40 ng/mL) and the limit of detection (LOD, 7.5 ng/mL) values were improved by a factor of 45 over the previously described FPIA using polyclonal antibodies. This technique was tested by the recovery method, and the high percentage of DBP discovery in water ranged from 85 to 110%. Using the developed method, real water samples from Lake Onega were tested, and a good correlation was shown between the results of the determination of DBP by the FPIA method and GC-MS. Thus, the FPIA method developed in this work can be used to determine DBP in open-water reservoirs.

show abstract

Section: Determination Of Dbp In Water Samples By Gc-msmentioning

confidence: 99%

Section: Determination Of Dbp In Water Samples By Gc-msmentioning

confidence: 99%

Section: Determination Dbp In Real Samplesmentioning

confidence: 99%

Section: Determination Dbp In Real Samplesmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay

Mukhametova,

Karimova,

Zharikova

et al. 2023

Biosensors

View full text Add to dashboard Cite

show abstract

“…The determination was carried out by gas chromatography-mass spectrometry in the mode of registration of isolated ions. [26,27]. Briefly, the column HP-5MS (30m×250 microns×0.25 microns) ("Agilent Technologies", USA) was used, mobile phase -helium, eluent flow rate of 1 ml/min.…”

Section: Determination Of Dbp In Water Samples By Gc-msmentioning

confidence: 99%

Detection of Di-Butyl Phthalate in Surface Water by Fluorescence Polarization Assay and Gas Chromatography -Mass Spectrometry

Mukhametova,

Karimova,

Zharikova

et al. 2023

Preprint

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Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to give them flexibility, strength and extensibility. However, due to its negative impact on human health, in particular, reproductive function and fetal development, the content of DBP must be controlled in food and environmental objects. The present study aims to develop a sensitive, fast and simple polarization fluorescent immunoassay (FPIA) using monoclonal antibodies derived against DBP (MAb-DBP) for its detection in open waters. New conjugates of DBP with various fluorescein derivatives were obtained and characterized: 5-aminomethylfluorescein (AMF) and dichlorotriazinylaminofluorescein (DTAF). The advantages of using the DBP-AMF conjugate in the FPIA method are shown, the kinetics of binding of this drug with antibodies is studied, the analysis is optimized, and the concentration of monoclonal antibodies is selected for sensitive analysis - 16 nM. The calibration dependence of the fluorescence polarization signal for the detection of DBP was obtained. The observed IC50 (40 ng/mL) and LOD (10 ng/mL) values were improved by a factor of 35 for the previously described FPIA using polyclonal antibodies. This technique was tested by the added/found method, the percentage of DBP discovery in water ranged from 85 to 110%. Using the developed method, real water samples from Lake Onega were tested, and a good correlation was shown between the results of the determination of DBP by the FPIA method and GC-MS/MS. Thus, the FPIA method developed in this work can be used to determine DBP in open water reservoirs.

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Application of microemulsions for the extraction of o-phthalate esters from soil with subsequent separation of microemulsions, simultaneous preconcentration, and gas chromatographic analysis of target components

Tolmacheva

Пирогов

Шпигун

2017

Moscow Univ. Chem. Bull.

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Extraction and subsequent determination of dialkyl phthalates in the soil using gas chromatography combined with tandem mass spectrometry

Cited by 3 publications

References 6 publications

Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay

Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay

Detection of Di-Butyl Phthalate in Surface Water by Fluorescence Polarization Assay and Gas Chromatography -Mass Spectrometry

Application of microemulsions for the extraction of o-phthalate esters from soil with subsequent separation of microemulsions, simultaneous preconcentration, and gas chromatographic analysis of target components

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