Extraction‐Fermentation Hybrid (Extractive Fermentation)

Yang, Shang‐Tian; Lu, Congcong

doi:10.1002/9781118493441.ch15

Cited by 18 publications

(11 citation statements)

References 142 publications

(139 reference statements)

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“…However, the coproduction of acetic acid with butyric acid in the fermentation not only results in a relatively low butyric acid yield and purity (selectivity) but also a high recovery cost due to difficulty in separating butyric acid from acetic acid (Fu, Wang, et al, 2017; Zigova et al, 1999). Although metabolic engineering to reduce acetate and increase butyrate biosynthesis (X. G. Liu & Yang, 2006; X. G. Liu et al, 2006; Zhu et al, 2005) and in situ product separation through extractive fermentation (Wu & Yang, 2003; Yang & Lu, 2013; Zigova & Sturdik, 2000) have been successfully used to improve the final concentration, productivity, and yield of butyric acid produced in fermentation by C. tyrobutyricum , how to eliminate or significantly reduce acetic acid production in the fermentation remains a challenge (Jiang et al, 2018; Luo et al, 2018; Zhang, Yang, et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum

Meng

Tang³

et al. 2020

Biotech & Bioengineering

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Clostridium tyrobutyricum produces butyric and acetic acids from glucose. The butyric acid yield and selectivity in the fermentation depend on NADH available for acetate reassimilation to butyric acid. In this study, benzyl viologen (BV), an artificial electron carrier that inhibits hydrogen production, was used to increase NADH availability and butyric acid production while eliminating acetic acid accumulation by facilitating its reassimilation. To better understand the mechanism of and find the optimum condition for BV effect on enhancing acetate assimilation and butyric acid production, BV at various concentrations and addition times during the fermentation were studied. Compared with the control without BV, the addition of 1 μM BV increased butyric acid production from glucose by ∼50% in yield and ∼29% in productivity while acetate production was completely inhibited. Furthermore, BV also increased the coutilization of glucose and exogenous acetate for butyric acid production. At a concentration ratio of acetate (g/L) to BV (mM) of 4, both acetate assimilation and butyrate biosynthesis increased with increasing the concentrations of BV (0–6.25 μM) and exogenous acetate (0–25 g/L). In a fed‐batch fermentation with glucose and ∼15 g/L acetate and 3.75 μM BV, butyrate production reached 55.9 g/L with productivity 0.93 g/L/h, yield 0.48 g/g, and 97.4% purity, which would facilitate product purification and reduce production cost. Manipulating metabolic flux and redox balance via BV and acetate addition provided a simple to implement metabolic process engineering approach for butyric acid production from sugars and biomass hydrolysates.

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Section: Introductionmentioning

confidence: 99%

Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum

Meng

Tang³

et al. 2020

Biotech & Bioengineering

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“…The constant recovery of toxic products lowers the actual concentration in the culture broth [ 21 ]. That way, product inhibition is decreased, leading to increased solvent yields and productivities and improved substrate conversion rates [ 164 , 273 , 325 , 328 ].…”

Section: Continuous Fermentation Methodsmentioning

confidence: 99%

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Vees

Neuendorf

Pflügl

2020

Journal of Industrial Microbiology and Biotechnology

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The sustainable production of solvents from above ground carbon is highly desired. Several clostridia naturally produce solvents and use a variety of renewable and waste-derived substrates such as lignocellulosic biomass and gas mixtures containing H2/CO2 or CO. To enable economically viable production of solvents and biofuels such as ethanol and butanol, the high productivity of continuous bioprocesses is needed. While the first industrial-scale gas fermentation facility operates continuously, the acetone–butanol–ethanol (ABE) fermentation is traditionally operated in batch mode. This review highlights the benefits of continuous bioprocessing for solvent production and underlines the progress made towards its establishment. Based on metabolic capabilities of solvent producing clostridia, we discuss recent advances in systems-level understanding and genome engineering. On the process side, we focus on innovative fermentation methods and integrated product recovery to overcome the limitations of the classical one-stage chemostat and give an overview of the current industrial bioproduction of solvents.

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“…Liquid–liquid equilibrium of systems where an organic acid splits between an aqueous phase and an organic phase has been widely studied to test new and efficient extractive processes for removal and purification of organic acids to reduce the cost of the final separation steps for the process . Long-chain aliphatic amines have proved to be efficient for organic acid extraction from diluted solutions, − where tertiary amines have been most used and tested in several liquid–liquid equilibria with organic acids such as gallic, tartaric, butyric, and lactic acids, among others.…”

Section: Introductionmentioning

confidence: 99%

Liquid–Liquid Equilibria of Lactic Acid/Water Solutions in Tri-iso-octylamine/Dodecane/1-Dodecanol at 306.1, 310.1, and 316.1 K. Experimental Data and Prediction

2019

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The liquid–liquid equilibria of systems that involves lactic acid in the aqueous phase and tri-iso-octylamine with diluents as dodecane and 1-dodecanol (active or/and inert) were measured experimentally at three temperatures (306.15, 310.15, and 316.15 K). A previous liquid–liquid equilibrium model that is based on Nernst’s distribution law and mass action law equilibrium equations was extended and generalized for stoichiometric ratios (amine/acid) 1:n. The effect of the diluents and the tertiary amine on the liquid–liquid equilibrium is shown and quantified in terms of the predicted values of the distribution coefficient, chemical equilibrium constants, and temperature. The lactic acid concentration in equilibrium for the organic phase decreases as follows: water/LA/TiOA/1-dodecanol system > water/LA/TiOA/dodecane/1-dodecanol > system water/LA/TiOA/dodecane system.

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Extraction‐Fermentation Hybrid (Extractive Fermentation)

Cited by 18 publications

References 142 publications

Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum

Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Liquid–Liquid Equilibria of Lactic Acid/Water Solutions in Tri-iso-octylamine/Dodecane/1-Dodecanol at 306.1, 310.1, and 316.1 K. Experimental Data and Prediction

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