Extraction, Fractionation, And Antioxidant Examination Of Polyfloral Honey Originated From Bone Prefecture South Sulawesi Province

Abstract

The aim of this research to isolate and identify and to test the antioxidant activity of extract and fraction of polyfloral honey originated from Bone regency, South Sulawesi. The results showed that methanol gave results in flavonoid, tannin, saponnin, steroid, and alkaloid with IC50 value of DPPH method of 683,153 µg/mL. DCM extract gave positive results for tannin, steroid and alkaloid with IC50 values of 701,743 µg/mL. n-hexane extract positively contains tannin and alkaloid with IC50 1709,536 µg/Ml, water extract positevely contains tannin, sapponin steroid, alkaloid with IC50 value 1698,345 µg/mL. Pure honey contains all aspects tested with IC50 values 2826,471 µg/mL. This showed extracts and sample have weak antioxidant activity.

Antibacterial Activity and Toxicity of Honey Derived from Bone, South Sulawesi, Indonesia

“…The high toxicity of methanol crude extract against Artemia salina when compared to ethyl acetate fraction and methanol fraction may be due to a large number of compounds in the methanol crude extract, resulting in a synergistic effect. This theory is supported by Mandey et al (2019) who reported that both original honey and methanol crude extract of the honey contains all the major compounds of secondary metabolites such as saponins, steroids, alkaloids, flavonoids, and tannins. (Mandey et al, 2019), different from methanol fraction, which is the remainder of the separation of the n-hexane and ethyl acetate fractions.…”

“…This theory is supported by Mandey et al (2019) who reported that both original honey and methanol crude extract of the honey contains all the major compounds of secondary metabolites such as saponins, steroids, alkaloids, flavonoids, and tannins. (Mandey et al, 2019), different from methanol fraction, which is the remainder of the separation of the n-hexane and ethyl acetate fractions. These results are also in line with studies that have been reported by Astuti et al (2019), which show that methanol extract by macerated has the highest toxicity (55 µg/ml) than ethanol (105 µg/ml) and water extract (47241 µg/ml) using brine shrimp lethality assay (Astuti et al, 2019).…”

“…Methanol crude extract of the tested honey showed the greatest inhibition compared to the fraction. This may be due to compound content which still has many compounds, such as polar and non polar compounds (Mandey, Handayani, Nanda, & Noor, 2019). While, the n-hexane fraction, based on other research, only have tannin and alkaloid, making it have poor antibacterial activity than other fraction (Mandey et al, 2019).…”

“…The inhibition zone of methanol crude extract against S. aureus was 11.90 mm and E. coli was 10.10 mm at a concentration of 1000 µg/ml. Methanol crude extract still contains non polar and polar compounds (Mandey et al, 2019), while methanol fraction only contains only some compounds because it has been partitioned with n-hexane and ethyl acetate. Based on research conducted by Vaquero et al (2007) (Vaquero, Alberto, & de Nadra, 2007), this diameter shows that the tested honey has strong antimicrobial activity against both gram-positive and gram-negative.…”