Extraction, Fractionation and Characterization of Cotton (<i>Gossypium herbaceum</i> L.) Seed Proteins

Gandhi, Kelvin; Litoriya, Nitesh S; Shah, Avadh K.; Talati, J. G.

doi:10.5958/0974-4479.2017.00003.x

Cited by 5 publications

(2 citation statements)

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“…These were followed by albumins (20.8~32.2% and 23.3~29.7%) and glutelins (9.1~24.5% and 19.6~28.0%), respectively, in G. arboreum and G. hirsutum lines; prolamins being the lowest in both the lines. The pattern of distribution of albumin and globulin fractions in the seed protein was found in contradiction with the results of Gandhi et al (2017) and Sammour et al (1995) who reported albumins with 40 to 50% as the major dominating fraction followed by globulins with 21 to 42% in lines of G. herbaceum and G. barbadense. Like seed protein content as discussed earlier, the proportion of protein fractions and their individual polypeptides could also be altered under different conditions of genetic set up, plant nutrients and other growth conditions.…”

Section: Discussioncontrasting

confidence: 86%

Comparative studies on seed protein characteristics in eight lines of two Gossypium species

Singh

Kaur

2019

J Cotton Res

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Background: In order to achieve the targets aiming at the improvement of protein quality, knowledge regarding seed protein fractions and polypeptides constituting them in different crops is essential. Besides having high nutritional value as animal feed and human food, the protein isolates from cottonseed meal have also been proven promising as industrial raw materials for a number of applications. As far as Indian work on the characterization of cotton seed proteins is concerned, relatively meagre reports are available. Keeping in mind the importance of cotton seed proteins, lines belonging to Gossypium arboreum L. (Indian cotton) and G. hirsutum L. (American cotton) which are grown in all the major cotton growing states in India were selected for analysing their seed protein characteristics. Results: Whereas G. arboreum (A-genome) lines revealed a lower range of seed protein content i.e. 19.5~24.3%, an upper range (21.8~29.5%) could be observed in lines of G. hirsutum (AD-genome). Globulins represented dominating fraction in both species followed by albumins, glutelins and prolamins. A significant positive correlation between albumins/globulins and seed protein content in G. arboreum /G. hirsutum, respectively, was observed. Intraspecific electrophoretic variation in seed protein extracts was observed in the region of molecular weight 22 kDa-27 kDa in lines of both the species; however some lines with A-genome showed similarity in banding pattern with AD-genome. Four polypeptides with disulphide-linkages were also reported for the first time. Albumins were observed to reveal more variations in their electrophoretic pattern between the lines of two species followed by globulins. Conclusion: On the basis of present and previous studies, screening the lines with low or high protein content will lead the selection of lines with superior polypeptide fraction important for nutritional and industrial purposes. On comparing the composition and behaviour of four 2-S linked polypeptides with other plant groups, these were suggested to be legumin-like in nature. The similarity in banding patterns between the lines of A-genome and AD-genome species marked towards the close evolutionary relationship between these two. Albumin fractions on the basis of our results could be taken for cultivar differentiation in cotton crop.

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Section: Discussioncontrasting

confidence: 86%

Comparative studies on seed protein characteristics in eight lines of two Gossypium species

Singh

Kaur

2019

J Cotton Res

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“…SPI and CPI showed higher levels of large proteins (>37 kDa), while the majority of proteins in IPPI and RPI were <37 kDa. Observed proteins likely correspond with fractions of various albumins and globulins, which are the largest fractions of seed storage proteins, and many of which exist in the size ranges observed for OPIs [17][18][19][20][21][22] . Ultimately, these results show that despite the use of the same extraction method for each plant source, the resulting protein mixtures varied substantially, suggesting that functionality may differ between OPIs produced through the same method.…”

Section: Alkali Extraction Of Protein Meals Enables Simple and Low-co...mentioning

confidence: 94%

A Beefy-R culture medium: replacing albumin with rapeseed protein isolates

Stout

Rittenberg

Shub

et al. 2022

Preprint

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The development of cost-effective serum-free media is essential for the economic viability of cultured meat. A key challenge facing this goal is high-cost recombinant albumin that is necessary in some available serum-free media formulations. As such, there is substantial interest in finding albumin alternatives which are low-cost, effective, scalable, sustainable, and suitable for food applications. Recently, a serum-free medium termed Beefy-9 was developed for bovine satellite cells (BSCs), which relied on recombinant albumin as a key component to replace fetal bovine serum. Here we alter Beefy-9 by replacing albumin with rapeseed protein isolate, a bulk-protein solution obtained from agricultural waste-streams through simple isoelectric protein precipitation. This new medium, termed Beefy-R, improves BSC growth compared with Beefy-9 while maintaining cell phenotype and myogenicity. These results offer an effective, low-cost, and sustainable alternative to albumin for serum-free culture of muscle stem cells, thereby addressing a key hurdle facing cultured meat production.

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