Extraction-free LAMP assays for generic detection of Old World Orthopoxviruses and specific detection of Mpox virus

Li, Zhiru; Sinha, Amit; Zhang, Yinhua; Tanner, Nathan; Cheng, Hui-Ting; Premsrirut, Prem; Carlow, Clotilde K. S.

doi:10.1038/s41598-023-48391-z

Cited by 5 publications

(1 citation statement)

References 49 publications

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“…The need for DNA extraction, cold chain transport, and storage of molecular reagents hinder the deployment of isothermal amplification tests to the field settings. However, recent innovations have enabled development of lyophilized isothermal amplification reagents to un-necessitate the need for cold chain transport system, and recent publication on extraction free LAMP assays further show the potential of isothermal amplification assays to be used for disease detection in limited resource settings [ 62 , 63 ].…”

Section: Introductionmentioning

confidence: 99%

Schistosomiasis diagnosis: Challenges and opportunities for elimination

Ally,

Kanoi,

Ochola

et al. 2024

PLoS Negl Trop Dis

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Overview The roadmap adopted by the World Health Organization (WHO) for eliminating neglected tropical diseases aims to eliminate schistosomiasis, as a public health concern, by 2030. While progress has been made towards reducing schistosomiasis morbidity control in several sub-Saharan African countries, there is still more that needs to be done. Proper surveillance using accurate diagnostics with acceptable sensitivity and specificity is essential for evaluating the success of all efforts against schistosomiasis. Microscopy, despite its low sensitivity, remains the gold standard approach for diagnosing the disease. Although many efforts have been made to develop new diagnostics based on circulating parasite proteins, genetic markers, schistosome egg morphology, and their paramagnetic properties, none has been robust enough to replace microscopy. This review highlights common diagnostic approaches for detecting schistosomiasis in field and clinical settings, major challenges, and provides new and novel opportunities and diagnosis pathways that will be critical in supporting elimination of schistosomiasis. Methods We searched for relevant and reliable published literature from PubMed, Scopus, google scholar, and Web of science. The search strategies were primarily determined by subtopic, and hence the following words were used (schistosom*, diagnosis, Kato–Katz, antibody test, circulating antigen, POC-CCA, UCP-LF-CAA, molecular diagnostics, nucleic acid amplification test, microfluidics, lab-on a disk, lab-on chip, recombinase polymerase amplification (RPA), LAMP, portable sequencer, nanobody test, identical multi-repeat sequences, diagnostic TPPs, REASSURED, extraction free), and Boolean operators AND and/OR were used to refine the searching capacity. Due to the global public health nature of schistosomiasis, we also searched for reliable documents, reports, and research papers published by international health organizations, World Health Organization (WHO), and Center for Disease control and Elimination.

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Section: Introductionmentioning

confidence: 99%

Schistosomiasis diagnosis: Challenges and opportunities for elimination

Ally,

Kanoi,

Ochola

et al. 2024

PLoS Negl Trop Dis

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Rapid and highly sensitive colorimetric LAMP assay and integrated device for visual detection of monkeypox virus

Peng,

Hu,

Xue

et al. 2024

Analytica Chimica Acta

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CRISPR-based strategies for sample-to-answer monkeypox detection: current status and emerging opportunities

Ahamed,

Politza,

Liu

et al. 2024

Nanotechnology

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The global health threat posed by the Monkeypox virus (Mpox) requires swift, simple, and accurate detection methods for effective management, emphasizing the growing necessity for decentralized point-of-care (POC) diagnostic solutions. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), initially known for its effective nucleic acid detection abilities, presents itself as an attractive diagnostic strategy. CRISPR offers exceptional sensitivity, single-base specificity, and programmability. Here, we reviewed the latest developments in CRISPR-based POC devices and testing strategies for Mpox detection. We explored the crucial role of genetic sequencing in designing crRNA for CRISPR reaction and understanding Mpox transmission and mutations. Additionally, we showed the integration of CRISPR-Cas12 strategy with pre-amplification and amplification-free methods. Our study also focused on the significant role of Cas12 proteins and the effectiveness of Cas12 coupled with recombinase polymerase reaction (RPA) for Mpox detection. We envision the future prospects and challenges, positioning CRISPR-Cas12-based POC devices as a frontrunner in the next generation of molecular biosensing technologies.

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Extraction-free LAMP assays for generic detection of Old World Orthopoxviruses and specific detection of Mpox virus

Cited by 5 publications

References 49 publications

Schistosomiasis diagnosis: Challenges and opportunities for elimination

Schistosomiasis diagnosis: Challenges and opportunities for elimination

Rapid and highly sensitive colorimetric LAMP assay and integrated device for visual detection of monkeypox virus

CRISPR-based strategies for sample-to-answer monkeypox detection: current status and emerging opportunities

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