Extraction, Isolation and Identification of Useful Phyto Constituents from Dichloromethane Leave Extract of Abutilon Pannosum and Grewia Tenax Using Q-TOF LC/MS

doi:10.20431/2349-0403.0410001

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“…Compounds with anti-diabetic potential, viz., cyclopentadeca-4, 12-dienone from Grewia hirsute have been reported by Abirami 21. Tridecanoic acid, Octanoic acid, Eicosanoic acid and Octadecatrienoic acid from Grewia tenax have been reported by Mital 22…”

Section: Introductionmentioning

confidence: 86%

<p>Isolation, pharmacological evaluation and molecular docking studies of bioactive compounds from Grewia optiva</p>

Bari¹,

Zahoor²,

Zeb³

et al. 2019

DDDT

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BackgroundTraditionally, Grewia optiva is widely used for the treatment of many diseases like dysentery, fever, typhoid, diarrhea, eczema, smallpox, malaria and cough.MethodsShade-dried roots of G. optiva were extracted with methanol. Based on HPLC results, chloroform and ethyl acetate fractions were subjected to silica column isolation and four compounds: glutaric acid (V), 3,5 dihydroxy phenyl acrylic acid (VI), (2,5 dihydroxy phenyl) 3',6',8'-trihydroxyl-4H chromen-4'-one (VII) and hexanedioic acid (VIII) were isolated in pure form. Ellman’s assay was used to determine the anticholinesterase potential of isolated compounds while their antioxidant potential was estimated by DPPH and ABTS scavenging assays.ResultsAmongst the isolated compounds, VI and VII exhibited excellent percent inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) (83.23±1.11, 82.72±2.20 and 82.11±2.11, 82.23±1.21, respectively, at 1000 µg/mL) with IC50 of 76, 90, 78 and 92 µg/mL, respectively. Highest percent radicals scavenging against DPPH and ABTS (87.41±1.20 and 86.13±2.31) with IC50 of 64 and 65 µg/mL, respectively, were observed for compound VII. Molecular docking studies also supported the binding of compound VI and VII with the target enzyme. The para-hydroxyl group of the phenolic moiety is formed hydrogen bonds with the active site water molecule and the side chain carbonyl and hydroxyl residues of enzyme.ConclusionThe isolated compounds inhibited the DPPH and ABTS-free radicals, and AChE and BChE enzymes. It was concluded that these compounds could be used in relieving the oxidative stress and pathological symptoms associated with excessive hydrolysis of acetyl and butyryl choline. The results of the study were supported by docking studies for compounds VI and VII.

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Section: Introductionmentioning

confidence: 86%