Extraction method for preparing pyridylamino sugar derivatives and application to porcine gastric mucus glycoprotein analysis

Down-regulation of the α-Gal Epitope Expression inN-Glycans of Swine Endothelial Cells by Transfection with theN-Acetylglucosaminyltransferase III Gene

“…To this solution, 200 l of a reducing reagent, 39 mg of borane-dimethylamine complex (Wako Pure Chemicals) in 200 l of glacial acetic acid, which was prepared just prior to use, was added, and the resulting solution was heated at 80°C for 50 min. Excess reagents were removed by benzene extraction and gel filtration, as described previously (44,45). The resulting pyridylaminated (PA) oligosaccharides were further purified using a Cellulose Cartridge Glycan preparation kit (Takara) according to the procedures recommended by the supplier.…”

Recent Developments in Liquid Chromatography and Capillary Electrophoresis for the Analysis of Glycoprotein Glycans

“…2-AP reaction mixtures are purified using a gel filtration column (TSK-gel HW-40F, 1.3 × 20 cm) with recoveries of approximately 80%. 53,54 Ion-exchange columns 55 and solvent extraction 56 can be used for smaller oligosaccharides. A labeling reaction mixture of 8-aminopyrene-1,3,6-trisulfonic acid (APTS) can be purified by gel filtration using a Sephadex G10 column.…”

Structural Analysis of O-Linked Oligosaccharide-Alditols by Electrospray–Tandem Mass Spectrometry after Mild Periodate Oxidation and Derivatization with 2-Aminopyridine

“…The reaction mixture was vortexed and heated at 80°C for 60 min and then cooled to room temperature. To remove the borane-pyridine complex, 75 and 85% methanol was added in succession to the reaction mixture and evaporated (58). After addition of 500 l of deionized water, the pH of the solution was adjusted to 10 with diluted ammonia solution and the excess of reagents was extracted with 500 l of chloroform (eight times).…”