Extraction of antioxidant and antimicrobial compounds from Inga marginata Willd bark and pulp using different extraction techniques and phytochemical characterization

Flores, Déborah Cristina Barcelos; Boeira, Caroline Pagnossim; Weis, Grazielle Castagna Cezimbra; Mello, Rosâne; Reis, Frederico Luiz; Morandini, Liziane Maria Barassuol; Morel, Ademir F.; Santos, Davi Teixeira dos; Flores, Érico Marlon de Moraes; Záchia, Renato Aquino; Nogueira-Librelotto, Daniele Rubert; Rolim, Clarice Madalena Bueno; Rosa, Cláudia Severo da

doi:10.1016/j.ifset.2022.103244

Cited by 3 publications

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies have shown that plant extracts have antibacterial and antifungal activities, which may be related to the presence of bioactive compounds (Gökbulut et al., 2013 ), (Flores et al., 2023 ). Therefore, in this study, the disk diffusion test was designed to determine the potential of MAE–IHRE and maceration–IHRE to inhibit the growth of some strains.…”

Section: Resultsmentioning

confidence: 99%

Microwave‐assisted extraction optimization of sesquiterpene lactones from Inula helenium roots: A sustainable approach to reduce energy consumption and carbon footprint

Özcan,

Dikmen,

Özcan

et al. 2023

Food Science & Nutrition

View full text Add to dashboard Cite

Inula helenium roots are consumed as natural flavor components and raw or cooked as food, and their extracts are rich in sesquiterpene lactones such as alantolactone (AL) and isoalantolactone (IAL), which have recently attracted great attention due to their pharmacological properties. The industrial utilization of these compounds requires the development of green, efficient, cost‐effective, and sustainable extraction protocols. Therefore, this study focused on the optimization of microwave‐assisted extraction (MAE) process variables using Face‐Centered Central Composite Design (FC‐CCD). Then, maceration was applied as a conventional technique, and these techniques were compared in terms of extraction efficiency, morphological changes, antimicrobial activities, carbon emissions, and energy consumption. As a result, optimal MAE conditions, i.e., EtOH: water ratio (X1) = 100:0, liquid/sample ratio (X2) = 30:1 mL/g, microwave power (X3) = 300 W, and irradiation time (X4) = 5 min, were obtained with AL and IAL yields of 54.99 ± 0.11 (mg/g) and 48.40 ± 0.19 (mg/g), respectively. The extract obtained by MAE had similar or better activity than positive controls in most cases and formed the largest inhibition zones against E. coli (29.5 ± 0.71 mm) and A. niger (34.75 ± 1.06 mm). Morphological changes of I. helenium roots after extraction were observed by scanning electron microscopy. Additionally, MAE was 43.4 times faster than maceration, resulting in 228.6 times less energy consumption and carbon emissions. Based on these findings, it is recommended to use MAE as an industrial green technique for the extraction of sesquiterpene lactones with potential applications in nutraceuticals and food products in terms of sustainable economy and environmental protection.

show abstract

Section: Resultsmentioning

confidence: 99%

Microwave‐assisted extraction optimization of sesquiterpene lactones from Inula helenium roots: A sustainable approach to reduce energy consumption and carbon footprint

Özcan,

Dikmen,

Özcan

et al. 2023

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

A comparison of phytoconstituent and functional loaded low moisture food from Averrhoa bilimbi using freeze drying and oven drying methods

Yusoff,

Azelee,

Chua

et al. 2024

Food Measure

View full text Add to dashboard Cite

Extraction of Sesquiterpene Lactones from Inula helenium Roots by High-Pressure Homogenization and Effects on Antimicrobial, Antioxidant, and Antiglycation Activities

Özcan,

Dikmen Meral

et al. 2024

Food Bioprocess Technol

View full text Add to dashboard Cite

The sesquiterpene lactones (SL) of Inula helenium (I. helenium) roots were extracted using high-pressure homogenization (HPH) and compared against those derived from maceration. The effect of process conditions on the extraction efficiency of bioactive compounds (alantolactone, isoalantolactone, total phenols, and flavonoids); the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging activity, ferric-reducing antioxidant power (FRAP), and antimicrobial activity of the extract; and the inhibition of advanced glycated end product (AGE) formation were assessed. The HPH of 90 MPa for 4 passes demonstrated the highest alantolactone (38.1 ± 0.7 mg/g) and isoalantolactone yields (34.4 ± 0.2 mg/g), respectively, which were significantly higher than the rates obtained using maceration (20.0 ± 0.2 mg/g and 18.9 ± 0.7 mg/g). Under optimal extraction conditions of 90 MPa for 4 passes, inhibition of AGE formation in the extract reached a rate of 97.16 ± 1.86%, with the highest DPPH and FRAP of 231.63 ± 2.96 µg/mL and 949.43 ± 1.86 µmol TE/100 mL, respectively. HPH exhibited lethal activity against all tested bacteria and fungi strains (Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Aspergillus niger, Fusarium oxysporum, and Penicillum expansum) at a concentration of 15 µL. SEM results proved that HPH severely damaged the cell structures of roots, increased solvent permeability, and improved the extraction rate of bioactive compounds. Additionally, this study also demonstrated that HPH yields high recovery with a specific energy consumption of 0.22 kWh/kg, which is close to the recommended minimum energy demand for an extraction procedure within industrial production processes. Therefore, these findings showed that HPH can be used as an efficient extraction technique for the production of SL in related industries.

show abstract

Extraction of antioxidant and antimicrobial compounds from Inga marginata Willd bark and pulp using different extraction techniques and phytochemical characterization

Cited by 3 publications

References 34 publications

Microwave‐assisted extraction optimization of sesquiterpene lactones from Inula helenium roots: A sustainable approach to reduce energy consumption and carbon footprint

Microwave‐assisted extraction optimization of sesquiterpene lactones from Inula helenium roots: A sustainable approach to reduce energy consumption and carbon footprint

A comparison of phytoconstituent and functional loaded low moisture food from Averrhoa bilimbi using freeze drying and oven drying methods

Extraction of Sesquiterpene Lactones from Inula helenium Roots by High-Pressure Homogenization and Effects on Antimicrobial, Antioxidant, and Antiglycation Activities

Contact Info

Product

Resources

About