Extraction of Antioxidants fromAronia mitschuriniiJuice Using Macroporous Resins

Abstract

Antioxidants play a vital role in the human body by defending cells from damage caused by free radicals, highly reactive products of oxidation reactions. A major source of antioxidants is fruits and vegetables. Aronia mitschurinii , a breed created at the end of the 19th century by crossbreeding wild Aronia melanocarpa and Russian Mountain Ash, produces fruits with one of the highest known content of hydrophilic antioxidants. Aronia fruit contains a potent blend of anthocyanins, polyphenols, and flavonoids. The most popular way of consuming the fruit is through juicing. Yet, due to very high concentrations of tannins in the juice, very few food-related applications have been developed. Resin extraction of antioxidants provides an alternative for utilizing valuable phytochemicals from crops for applications in the food industry as nutraceutical supplements and more. To increase the market value of the plant, it is important to determine what resins can extract the optimum concentration of antioxidants from aronia juice, pulp, and whole berries. We have shown that macroporous resins such as Amberlite XAD 1180N, Amberlite XAD 7HP, Amberlite XAD 761, and Amberlite FPX66, which have been reported to be effective in extracting the anthocyanins and polyphenols from other fruit juices, skins of red grapes, and the wild breed, are also effective for use in juice, pulp, and whole fruits of Aronia mitchurinii . However, the extremely high content of antioxidants presents a challenge to obtaining high recovery; a notable change in the juice/resin ratio is required to obtain a higher recovery value. Our results showed that Amberlite FPX66 was the best at extracting anthocyanins, polyphenols, and flavonoids from aronia juice. A separate experiment conducted to determine how to optimize the efficiency of FPX66 extraction revealed that increasing the resin/juice ratio increased the percent recovery of anthocyanins from aronia juice. Moreover, we have compared recovery between juice, pulp, and whole aronia berries and batch versus column extraction.

Mexican Native Black Bean Anthocyanin-Rich Extracts Modulate Biological Markers Associated with Inflammation

“…Delphinidin-3-glucoside, genistein, rutin, naringenin, catechin, myricetin, and rosmarinic acid were not found in REF2. Fractioning and purifying processes modified the phytochemical profile in each treatment as a consequence of the affinity of the molecules for the resin and the solvent phases in the HPCCC process [ 44 ]. Raw extract fractions (REF1, REF2) presented the lowest number of compounds compared to purified extract fractions (PEF1, PEF2), which showed most of the compounds contained in the original extract.…”

“…Moreover, the fractionation profile shows that quercetin-3-glucoside (Q3G) was present in all fractions, indicating its stability and solubility at different conditions. Similarly, kaempferol-3-glucoside was identified in the second fractions of the extracts (REF2 and PEF2), indicating that this compound interacted better with the stationary phase in HPCCC for both RE and PE [ 44 ]. At the same elution time in the HPCCC, quercetin-3-galactoside was present in PE, REF1, and PEF1.…”

Сок Черноплодной Рябины: 10 Веских Причин Добавить В Свой Рацион

“…Плоды черноплодки богаты сильнодействующими биологически активными соединениями и имеют один из самых мощных антиоксидантных составов. [15] Многочисленные научные эксперименты подтверждают, что сок аронии Мичурина весьма эффективен в борьбе с окислительным стрессом, вызванным гипергликемией, показывает хорошие результаты в терапии диабета 2-го типа. [16]…”

A theoretical study on radical scavenging activity of phenolic derivatives naturally found within Alternaria alternata extract

“…In order to reduce the excessive production of ROS, and then to avoid its adverse effects, the action of antioxidants plays an important role in protecting the cell from damage caused by the free radical. 11,12 They have the ability to scavenge free radicals owing to the presence of a number of OH groups. ROS are also produced through the activation of pro-oxidant enzymes like xanthine oxidase (XO) inside the peroxisomes.…”