Extraction of dried oral fluid spots (DOFS) for the identification of drugs of abuse using liquid chromatography tandem mass spectrometry (LC-MS/MS)

Gorziza, Roberta Petry; Cox, Jos A.; Limberger, Renata Pereira; Arroyo, Luis

doi:10.1016/j.forc.2020.100254

Cited by 17 publications

(28 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dried Matrix Spots (DMS) have been of interest in forensic toxicology (Chepyala et al 2017 ; Sadler Simões et al 2018 ; Caramelo et al 2019 ; Ribeiro et al 2019 ; Seymour et al 2019 ; Gorziza et al 2020 ), especially to simplify on-site sample collection while reducing time and resources. It consists of applying a small quantity of a biological sample (e.g., 50 µL) into a paper substrate, and set it to dry (Hannon and Therrell 2014 ; Resano et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…DMS are also designed for a simple and fast sample extraction, reducing resources cost and chemical waste (Déglon et al 2015 ). When coupled to sensitive detection instruments, such as liquid chromatography tandem mass spectrometry (LC–MS/MS), it can provide drug detection at lower concentrations (Gorziza et al 2020 ). THC identification and quantification have been shown in analytical methods using dried blood spots (DBS) (Thomas et al 2012 ; Mercolini et al 2013 ; Kyriakou et al 2016 ; Protti et al 2017 ), but THC isolation have been studied only for an extraction protocol using dried oral fluid spots (DOFS) (Stoykova et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the aim of this study is to include THC and CBD detection to an established DOFS sampling protocol and an extraction procedure (Gorziza et al 2020 ), which covered the identification and quantification of amphetamine, methamphetamine, benzoylecgonine, ketamine and mitragynine, using a LC–MS/MS instrument. To the best of our knowledge, CBD has been included for the first time in DMS, and it can help to evaluate cannabis medical use, among other scenarios.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) extraction FROM dried oral fluid spots (DOFS) and LC–MS/MS detection

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Oral fluid is a widely studied matrix able to isolate the primary Cannabis constituent THC, facilitating its detection via mass spectrometry, and in most cases link these findings to recent drug use. As an alternative to liquid oral fluid, dried oral fluid spots (DOFS) is a simple and a low-cost sampling technique. It has shown improved stability compared to liquid samples, allowing for the possibility to preserve the specimens under various temperature and humidity conditions. The sampling strategy is straightforward and involves the application of a small quantity of oral fluid aliquot to a paper substrate that is set to air dry allowing for on-site collection at a large-scale demand. The goal of this study is to study THC and CBD extraction from DOFS, applying a previous established protocol for a LC–MS/MS qualitative method validation. Although other drugs of abuse have been included in DOFS methods, this is the first method validation including cannabinoids. An alternative oral fluid extraction method (WAX-S tips) is demonstrated to improve the recovery of the analytes. Methods A pool of blank oral fluid was used to prepare THC and CBD spiked DOFS samples for method validation and application. Spiked oral fluid was used to demonstrate WAX-S tips THC and CBD extraction. All samples were analyzed on a LC–MS/MS instrument. Results The qualitative method validation for THC and CBD confirmation in DOFS included method selectivity, matrix effects (< 20%), recovery (average of 25%), process efficiency (average of 21%), LOD (2 ng/mL for THC and 4 ng/mL for CBD), absence of carryover, and DOFS stability (70% in 35 days) as figures of merit. The method application in blindly prepared samples demonstrated the method capability to identify THC and CBD. WAX-S tips extraction showed an average of 91% recovery of THC and CBD from liquid oral fluid. Conclusions THC and CBD extraction from DOFS showed low recoveries. However, the LC–MS/MS qualitative confirmation of THC and CBD in DOFS could improve cannabinoids screening in oral fluid, as it shows adequate LOD and stability over time. This method has potential for assisting the screening of drivers under possible drug influence by facilitating sample transportation and temporary storage in dried spot form. Additional research is suggested for WAX-S tips extraction and quantitative method validation.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Study of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) extraction FROM dried oral fluid spots (DOFS) and LC–MS/MS detection

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…No entanto, seu alto custo de aquisição e manutenção é limitado para laboratórios forenses que tenham orçamento compatível. Na literatura é possível encontrar diversos métodos desenvolvidos com o MS, ou in tandem (MS/MS) para determinação de MDMA, MDA, e estimulantes do tipoanfetamina em amostras de fluido oral (CONCHEIRO et al, 2008;SERGI et al, 2009;ROSITANO et al, 2016;JACQUES;DOS SANTOS;LIMBERGER, 2019;GORZIZA et al, 2020) e apreendidas (KHAJEAMIRI et al, 2011;COUCHMAN et al, 2019;TEÓFILO et al, 2020), com diversas variações no modo de preparo das amostras, desde simples diluições (dilution-and-shoot) a convencionais extrações líquido-líquido. Os limites de detecção e de quantificação reportados para ambos os analitos podem chegar a níveis na ordem de 0,2 e 0,5 ng mL -1 no fluido oral (WOOD et al, 2003) e até 0,0001 ng mL -1 para o cabelo.…”

Section: Cromatografia Líquida (Lc-dad Lc-fd E Lc-ms)unclassified

“…utilizou um sistema LC-HRMS como técnica de confirmação após amostras de OF serem coletadas com o dispositivo Drugwipe-5S (cut off de 100 ng mL -1 ), sendo os analitos extraídos com metanol. Outros trabalhos em DOFS são os deGorziza et al (2020) eRibeiro et al (2019). Ambos utilizam métodos baseados em MS/MS e possuem LDs tão baixos quanto 0,2 ng mL -1 para 50 µL de fluido oral.…”

unclassified

Dried Spots associado com um método LC-DAD para determinação simultânea de MDMA (ecstasy) e MDA em amostras de fluído oral

Duarte¹

View full text Add to dashboard Cite

O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) -Código de Financiamento 001.Devido ao espaço limitado desta seção que não me permite agradecer devidamente a todas as pessoas, deixo palavras de agradecimento, mas com um sentimento de profundo reconhecimento para aquelas que não citar aqui. Primeiramente, ao professor Marcelo, pela orientação, oportunidade e apoio nas escolhas realizadas durante meu ingresso e subsequentes estudos no mestrado, o meu sincero agradecimento pela confiança que depositou em mim e disponibilidade que sempre revelou para comigo. Ao professor José Fernando, por estar sempre à disposição, seja para sanar dúvidas, compartilhar experiências, ou até mesmo emprestar seus valiosos e queridos livros em prol do conhecimento. Ao professor Anderson, pelos ensinamentos e disponibilidade. Agradeço também pelas valiosas sugestões e doação de vários reagentes para que eu pudesse concluir o meu trabalho de mestrado.Ao Bruno, grande amigo e irmão que fiz neste período de pós-graduação. Meu enorme agradecimento pelos momentos de descontração, pela força, críticas, parceria, e a todos os bons momentos passados aos longos destes dois anos, inclusive nas longas, cansativas, mas divertidas horas de trabalho no laboratório. À Fernanda, por me auxiliar em vários momentos dentro da vida de pós-graduando, pelos conselhos, puxões de orelha e, claro, pelas amizades, boas conversas e risadas. Vocês são pessoas incríveis que merecem sempre o de melhor. Ao Guilherme, que me auxiliou em vários momentos para me enviar os dados que no computador estavam, e a todas as pessoas que fizeram parte do meu cotidiano durante esses anos em Ribeirão Preto e que, de alguma forma, compartilharam comigo momentos de alegria e conhecimento. A todos, o meu muito obrigado. À minha família, um enorme e sincero obrigado pelo apoio e por sempre acreditarem em mim, mesmo nos momentos mais difíceis. Obrigado, pai e mãe, por todos os sacrifícios que fizeram e fazem por mim. Não existem palavras o suficiente para demonstrar a minha gratidão.Não existem palavras que expressem o quanto os dois significam para mim. À minha querida e incrível Ana, que sempre teve paciência e que me apoiou desde que nos conhecemos. Agradeço imensamente pelo carinho, amor e companheirismo que tem me demonstrado. Você tem o dom de fazer as pessoas felizes e é um exemplo de superação.Obrigado por sempre acreditar em mim, obrigado pela transmissão de confiança e de força, em todos os momentos. Nos momentos mais difíceis procurei em você motivação para continuar e nunca me desapontei. Por tudo, a minha enorme gratidão. RESUMODUARTE, L. O. Dried Spots associado com um método LC-DAD para determinação simultânea de MDMA (ecstasy) e MDA em amostras de fluido oral. 2021. Dissertação (Mestrado em Ciências,

show abstract

Advancements in liquid chromatography‐mass spectrometry for amphetamine analysis in unconventional biological matrices: Focus on oral fluid and hair samples

Guizolfi,

Kramer,

Menck

et al. 2024

Separation Science Plus

View full text Add to dashboard Cite

Amphetamines (AMPHs) and their derivatives are potent central nervous system stimulants, in 2020 an astonishing 34 million regular users were reported worldwide. Consequently, there has been a critical need for the development of methods capable of detecting these compounds in alternative biological matrices. The review article discusses the analysis of amphetamines and their derivatives in alternative biological matrices, such as hair and oral fluid (OF), using liquid chromatography‐mass spectrometry. Compiles data from 28 articles published between 2012 and 2022, focusing on 13 articles on OFs and 15 on hair analysis. The article highlights the advantages of using these alternative matrices, including less invasive collection methods, greater sample stability, and simplified extraction processes. Additionally, prevalent analytical practices such as the use of C18 columns and the incorporation of formic acid and methanol into mobile phases are discussed. Furthermore, it demonstrates that the most used method for collecting OF is the Quantisal device, as well as solid phase extraction is also the most used for the same matrix. The review serves as a guide for researchers interested in analytical methods for identifying AMPHs, offering insights into advances, challenges, and the most used in the area.

show abstract

Extraction of dried oral fluid spots (DOFS) for the identification of drugs of abuse using liquid chromatography tandem mass spectrometry (LC-MS/MS)

Cited by 17 publications

References 49 publications

Study of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) extraction FROM dried oral fluid spots (DOFS) and LC–MS/MS detection

Study of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) extraction FROM dried oral fluid spots (DOFS) and LC–MS/MS detection

Dried Spots associado com um método LC-DAD para determinação simultânea de MDMA (ecstasy) e MDA em amostras de fluído oral

Advancements in liquid chromatography‐mass spectrometry for amphetamine analysis in unconventional biological matrices: Focus on oral fluid and hair samples

Contact Info

Product

Resources

About