Extraction of Gossypol from Cottonseed Meal

Abstract

The levulose equivalent of the dextrose, 140.7:12.4, is again 11.3 mg., so that the figures for levulose and dextrose, third approximation, are not changed further.The ratio between levulose and dextrose is thus found to be 98.7:140.7, or as 41:59. For this ratio Table IB gives a Lane and Eynon factor of 43.3 instead of 43.4, and the total reducing sugars are therefore not 239.4, but 238.8 mg. In this particular case a second calculation, on the basis of 238.8 mg. of total reducing sugars, is hardly necessary, because the difference is within the limits of error.If it is made, the second calculation gives 98.7 mg. of levulose and 140.1 mg. of dextrose in 100 ml. of solution or in 25.84 grams of raw sugar. The final result is therefore 0.382 per cent of levulose, 0.542 per cent of dextrose, 0.924 per cent of total reducing sugars.The results of the analyses of this and other raw sugars are compiled in Table IV.

Gossypol, a Pigment of Cottonseed.

“…Gossypol readily forms a highly insoluble crystalline compound with two molecules of aniline (45), a fact that has formed the basis of methods for estimating the gossypol content (both free and "bound") of cottonseed and cottonseed meal. The gravimetric procedures of Carruth (46) and Withers (230) for the conversion of gossypol into its dianiline derivative were employed by Sherwood (206) and Clark (51) and refined by Halverson and Smith (104,105,106,107,218,219). The methods employed by Royce and Kibler (190,193) for the determination of the gossypol content of crude cottonseed oils likewise depend upon the gravimetric determination of gossypol through the dianiline derivative.…”

Rapid Separation of Gossypol from Cottonseeds by a Solid-Phase Synthetic Method

“…The gossypol based polymeric drug Kagocel has been proven to be effective in numerous clinical tests for the treatment and prevention of influenza and herpes infections in adults and children.4-6 Total synthesis of gossypol has been achieved,7 and many methods for extraction and separation have been applied to purify it. [8][9][10][11] The shortcomings of these preparation methods include large requirements of time, high-cost, and the usage of toxic solvents. The chemical structure of gossypol is unstable as gossypol is air and light sensitive, so it is urgent to find a rapid method to purify it for the purpose ofmedical use or futher research.…”