Extraction of honokiol from Artemisia argyi and in vitro and in vivo investigation of its antifungal activity

Wang, Xianxian; Han, Xiaobin; Wang, Shu; Wang, Yaobin; Wang, Peng; Zhong, Zhao; Qin, Hao; Jing, Changliang; Liang, Chen

doi:10.1080/14786419.2022.2071887

Cited by 11 publications

(5 citation statements)

References 29 publications

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“…In this study, the inhibitory effects of seven phytochemicals against F. commune GUCCGF6 based on mycelial growth inhibition were studied with four phytochemicals found to have notable antifungal effects, and honokiol demonstrating the highest inhibition. Honokiol has been found to inhibit other fungi, such as Alternaria alternata and Rhizoctonia solani (Chen et al., 2019; Wang et al., 2022; Yan et al., 2020). The results of our pot study showed that honokiol, 2‐allylphenol, magnolol and carvacrol had significant control efficacy on rhizome rot of P. cyrtonema , but the antifungal mechanisms of these phytochemicals require further investigation.…”

Section: Discussionmentioning

confidence: 99%

Phytochemicals demonstrate potential fungicidal activity against Fusarium commune, a newly identified pathogenic fungus causing rhizome rot in Polygonatum cyrtonema (giant Chinese Solomon's seal)

Wei,

Zhao,

et al. 2024

Journal of Phytopathology

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Polygonatum cyrtonema (giant Chinese Solomon's seal) is a perennial herb belonging to the family Liliaceae, with significant medicinal and dietary value. However, a severe rhizome rot has led to a decline in the yield and quality of this species. In this study, a fungus was isolated from symptomatic rhizome samples and identified as Fusarium commune through molecular and morphological analyses. In pathogenicity tests, the identification of F. commune as the causative agent of rhizome rot was confirmed by fulfilling Koch's postulates. Biological characteristics indicated that the optimal growth conditions for F. commune were a temperature of 25°C, pH of 8, carbon source of soluble starch, nitrogen source of peptone and a photoperiod of 24 h light. In order to reduce the use of chemical fungicides and explore natural substances to control the disease, the sensitivity of F. commune to seven phytochemicals was studied. Four phytochemicals (honokiol, 2‐allylphenol, magnolol and carvacrol) showed inhibitory activity, with honokiol having a 50% of the maximal effect concentration (EC50) of 9.99 ± 0.14 mg/L. The results of pot experiment showed that the four phytochemicals had significant control efficacy on the rhizome rot of P. cyrtonema, and the highest control efficacy was observed with honokiol. These findings provide a scientific basis for the control of rhizome rot in P. cyrtonema.

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Section: Discussionmentioning

confidence: 99%

Phytochemicals demonstrate potential fungicidal activity against Fusarium commune, a newly identified pathogenic fungus causing rhizome rot in Polygonatum cyrtonema (giant Chinese Solomon's seal)

Wei,

Zhao,

et al. 2024

Journal of Phytopathology

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“…Moreover, aldehydes, alcohols, and phenolic compounds have shown certain antifungal activities in previous studies (Xie et al 2017;Lee et al 2010). Phytochemicals have a low risk of resistance development and high antifungal activity, and are considered safe and environmentally friendly..Honokiol has been found to inhibit other fungi, such as Alternaria alternata and Rhizoctonia solani (Chen et al 2019;Yan et al 2020;Wang et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Identification of a new root rot-causing pathogen in Polygonatum cyrtonema Hua and screening of phytochemicals for its control

Wei¹,

Wang²,

Zhao³

et al. 2023

Preprint

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Polygonatum cyrtonema Hua is a perennial herb belonging to the Liliaceae family, with significant medicinal and dietary value. However, root rot， a server disease, has led to a decline in yield and quality of this species. In this study, a fungus was isolated from symptomatic samples of root rot and identified as Fusarium commune through molecular and morphological analyses. After re-isolation, the identification of F. commune as the causative agent of root rot was confirmed by fulfilling Koch's postulates in subsequent tests.. Biological characteristics indicated that the optimal growth conditions for F. commune were a temperature of 25℃, pH of 8, carbon source of soluble starch, nitrogen source of peptone, and a photoperiod of 24 hours. In order to reduce the use of chemical fungicides and explore natural substances to control the disease, the sensitivity of F. commune to seven phytochemicals was studied. Four phytochemicals showed apparent inhibitory activity and honokiol displaying the highest antifungal activity, having a 50% of the maximal effect concentration (EC50) of 8.2628 ± 0.27 mg/L. These findings provide a scientific basis for the control of root rot in P. cyrtonema Hua.

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“…R. solanacearum RS10 was isolated from a diseased tobacco plant (Bai et al, 2016 ). The phytopathogenic fungi used in this study were Alternaria alternata, Phytophthora nicotianae, Botrytis cinerea, Fusarium oxysporum, Cucumber anthracnose , and Rhizoctonia cerealis (Xie et al, 2021 ; Wang et al, 2022 ). All strains were preserved in our laboratory and stored in 25% (v/v) glycerol at −80°C.…”

Section: Methodsmentioning

confidence: 99%

Biocontrol potential of Bacillus velezensis EM-1 associated with suppressive rhizosphere soil microbes against tobacco bacterial wilt

et al. 2022

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Tobacco bacterial wilt caused by Ralstonia solanacearum is one of the most devastating diseases. Microbial keystone taxa were proposed as promising targets in plant disease control. In this study, we obtained an antagonistic Bacillus isolate EM-1 from bacterial wilt-suppressive soil, and it was considered rhizosphere-resident bacteria based on high (100%) 16S rRNA gene similarity to sequences derived from high-throughput amplicon sequencing. According to 16S rRNA gene sequencing and MLSA, strain EM-1 was identified as Bacillus velezensis. This strain could inhibit the growth of R. solanacearum, reduce the colonization of R. solanacearum in tobacco roots, and decrease the incidence of bacterial wilt disease. In addition, strain EM-1 also showed a strong inhibitory effect on other phytopathogens, such as Alternaria alternata and Phytophthora nicotianae, indicating a wide antagonistic spectrum. The antimicrobial ability of EM-1 can be attributed to its volatile, lipopeptide and polyketide metabolites. Iturin A (C14, C15, and C16) was the main lipopeptide, and macrolactin A and macrolactin W were the main polyketides in the fermentation broth of EM-1, while heptanone and its derivatives were dominant among the volatile organic compounds. Among them, heptanones and macrolactins, but not iturins, might be the main potential antibacterial substances. Complete genome sequencing was performed, and the biosynthetic gene clusters responsible for iturin A and macrolactin were identified. Moreover, strain EM-1 can also induce plant resistance by increasing the activity of CAT and PPO in tobacco. These results indicated that EM-1 can serve as a biocontrol Bacillus strain for tobacco bacterial wilt control. This study provides a better insight into the strategy of exploring biocontrol agent based on rhizosphere microbiome.

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Extraction of honokiol from Artemisia argyi and in vitro and in vivo investigation of its antifungal activity

Cited by 11 publications

References 29 publications

Phytochemicals demonstrate potential fungicidal activity against Fusarium commune, a newly identified pathogenic fungus causing rhizome rot in Polygonatum cyrtonema (giant Chinese Solomon's seal)

Phytochemicals demonstrate potential fungicidal activity against Fusarium commune, a newly identified pathogenic fungus causing rhizome rot in Polygonatum cyrtonema (giant Chinese Solomon's seal)

Identification of a new root rot-causing pathogen in Polygonatum cyrtonema Hua and screening of phytochemicals for its control

Biocontrol potential of Bacillus velezensis EM-1 associated with suppressive rhizosphere soil microbes against tobacco bacterial wilt

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