Extraction of humic substances from fresh waters on solid-phase cartridges and their study by Fourier transform ion cyclotron resonance mass spectrometry

Zherebker, Alexander; Perminova, Irina V.; Konstantinov, Andrey I.; Volikov, Alexander B.; Kostyukevich, Yury; Kononikhin, Alexey; Nikolaev, E. N.

doi:10.1134/s1061934816040109

Cited by 20 publications

(18 citation statements)

References 15 publications

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“…2(c). 130,131 These results indicated that the higher proportion of hydrophobic alkyl carbon and aromatic carbon in plant residues, the less biodegradable, and the higher proportion of hydrophilic polysaccharides, the more easily degraded, usually completed in a few months. 132 Nowadays, an extensive effort has been devoted to understanding sorptive interactions between HOCs and natural DOM, particularly, the structure-activity correlations.…”

Section: Interaction Mechanism Of Cdom-soil-ocsmentioning

confidence: 97%

Structural characteristics, analytical techniques and interactions with organic contaminants of dissolved organic matter derived from crop straw: a critical review

et al. 2018

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Section: Interaction Mechanism Of Cdom-soil-ocsmentioning

confidence: 97%

Structural characteristics, analytical techniques and interactions with organic contaminants of dissolved organic matter derived from crop straw: a critical review

et al. 2018

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“…The FA fractions (FA1 and FA2) were isolated according to the modified solid‐phase extraction procedure as described elsewhere (Dittmar et al, ; Zherebker et al, ). In brief, a Bond Elut PPL cartridge (Megapack, 5 g) was activated by a consecutive discharge of 30 ml methanol and 30 ml 0.01M HCl prior to extraction.…”

Section: Methodsmentioning

confidence: 99%

The Molecular Composition of Humic Substances Isolated From Yedoma Permafrost and Alas Cores in the Eastern Siberian Arctic as Measured by Ultrahigh Resolution Mass Spectrometry

et al. 2019

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Ongoing climate change is making the large pool of organic matter (OM) stored in Arctic permafrost vulnerable to mobilization; thus, garnering a deeper understanding of molecular transformations within the abundant pool of soil OM, specifically humic substances, is crucial. Here we present the first high-resolution mass-spectrometry examination of molecular compositions of humic acid (HA) and fulvic acid (FA) isolated from organic-rich deep yedoma (Pleistocene age ice-rich permafrost) and alas (thermokarst deposit formed during permafrost thaw) cores. The FA fractions were dominated by oxygen-rich unsaturated compounds, whereas the HA fractions were mostly composed of relatively reduced saturated and aromatic moieties. A substantial increase in contribution of both CHO-only and N-containing aliphatic compounds was observed in the HA fractions of the yedoma OM with depth, whereas the alas HA fractions were depleted in aliphatics but enriched with condensed and hydrolyzable tannins. The observed differences in compositional space of the immobile OM stored in the deep yedoma versus alas deposits were consistent with evolution of OM during thermokarst lake genesis, implying intense microbial degradation of N-rich OM released from the yedoma deposits and accumulation of highly degraded, plant-derived OM. The patterns of molecular transformations of OM were apparent in compositional space of the least degraded HA fractions as compared to much more oxidized FA fractions. This shows great promise of molecular exploration of the alkali-extracted OM, comprising up to 50% of the total organic carbon in deep permafrost both for paleoreconstructions and predictions of climate feedback to released OM due to permafrost thaw.

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“…These clear outliers affected the number-averaged values given in Table 2. The data obtained by the a, c, and e laboratories were characterized by the dominant contribution of oxidized aromatic species with an O/C atomic ratio between 0.5 and 0.7, which is inherent for NOM from temperate and tropical regions [26,27]. The data sets of SRHA-b and SRHA-d did not show any distinct features.…”

Section: Intercomparison Of the Fticr Ms Data For The Srha Samplementioning

confidence: 96%

Interlaboratory comparison of humic substances compositional space as measured by Fourier transform ion cyclotron resonance mass spectrometry (IUPAC Technical Report)

Zherebker

Kim

Schmitt‐Kopplin

et al. 2020

Pure and Applied Chemistry

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Interlaboratory comparison on the determination of the molecular composition of humic substances (HS) was undertaken in the framework of IUPAC project 2016-015-2-600. The analysis was conducted using high resolution mass spectrometry, nominally, Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) with electrospray ionization. Six samples of HS from freshwater, soil, and leonardite were used for this study, including one sample of humic acids (HA) from coal (leonardite), two samples of soil HA (the sod-podzolic soil and chernozem), two samples of soil fulvic acids (FA) (the sod-podzolic soil and chernozem), and one sample of freshwater humic acids (the Suwannee River). The samples were analyzed on five different FTICR MS instruments using the routine conditions applied in each participating laboratory. The results were collected as mass lists, which were further assigned formulae for the determination of molecular composition. The similarity of the obtained data was evaluated using appropriate statistical metrics. The results have shown that direct comparison of discrete stoichiometries assigned to the mass lists obtained by the different laboratories yielded poor results with low values of the Jaccard similarity score – not exceeding 0.56 (not more than 56 % of the similar peaks). The least similarity was observed for the aromatics-rich HA samples from leonardite (coal) and the chernozem soil, which might be connected to difficulties in their ionization. The reliable similarity among the data obtained in this intercomparison study was achieved only by transforming a singular point (stoichiometry) in van Krevelen diagram into a sizeable pixel (a number of closely located stoichiometries), which can be calculated from the population density distribution. The conclusion was made that, so far, these are descriptors of occupation density distribution, which provide the metrics compliant with the data quality requirements, such as the reproducibility of the data measurements on different instruments.

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Extraction of humic substances from fresh waters on solid-phase cartridges and their study by Fourier transform ion cyclotron resonance mass spectrometry

Cited by 20 publications

References 15 publications

Structural characteristics, analytical techniques and interactions with organic contaminants of dissolved organic matter derived from crop straw: a critical review

Structural characteristics, analytical techniques and interactions with organic contaminants of dissolved organic matter derived from crop straw: a critical review

The Molecular Composition of Humic Substances Isolated From Yedoma Permafrost and Alas Cores in the Eastern Siberian Arctic as Measured by Ultrahigh Resolution Mass Spectrometry

Interlaboratory comparison of humic substances compositional space as measured by Fourier transform ion cyclotron resonance mass spectrometry (IUPAC Technical Report)

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