Extraction of intended palpation times from facial EMGs in a mouse model of active sensing

Schroeder, Joseph Bradley; Ritt, Jason T.

doi:10.1109/embc.2013.6609926

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Figure 4(a) shows an example of bilateral EMG RMS recordings (solid black traces) compared to 'gold-standard' whisker angles (dashed traces) estimated by manually tracking whisker angles in high speed video (500 frames s −1 ). We observed here and reported previously that EMG provides a reliable estimate of protraction times, as the EMG signal generally begins increasing prior to an observed increase in whisker angle [32].…”

Section: Resultssupporting

confidence: 80%

“…To facilitate closed-loop optogenetic feedback, we utilized facial electromyography (EMG) in the Thy1 (excitatory) mice described above to estimate whisker protractions events in real time as described previously [32]. An important advantage to OptoZIF Drive is its two part protective shell, which facilities EMG electrodes (or other secondary recordings) to be connected to the drive during the implant surgery prior to enclosing the drive.…”

Section: Resultsmentioning

confidence: 99%

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OptoZIF Drive: a 3D printed implant and assembly tool package for neural recording and optical stimulation in freely moving mice

et al. 2016

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Objective Behavioral neuroscience studies in freely moving rodents require small, light-weight implants to facilitate neural recording and stimulation. Our goal was to develop an integrated package of 3D printed parts and assembly aids for labs to rapidly fabricate, with minimal training, an implant that combines individually positionable microelectrodes, an optical fiber, zero insertion force (ZIF-clip) headstage connection, and secondary recording electrodes, e.g. for electromyograms (EMG). Approach Starting from previous implant designs that position recording electrodes using a control screw, we developed an implant where the main drive body, protective shell, and non-metal components of the microdrives are 3D printed in parallel. We compared alternative shapes and orientations of circuit boards for electrode connection to the headstage, in terms of their size, weight, and ease of wire insertion. We iteratively refined assembly methods, and integrated additional assembly aids into the 3D printed casing. Main Results We demonstrate the effectiveness of the OptoZIF Drive by performing real time optogenetic feedback in behaving mice. A novel feature of the OptoZIF Drive is its vertical circuit board, which facilities direct ZIF-clip connection. This feature requires angled insertion of an optical fiber that still can exit the drive from the center of a ring of recording electrodes. We designed an innovative 2-part protective shell that can be installed during the implant surgery to facilitate making additional connections to the circuit board. We use this feature to show that facial EMG in mice can be used as a control signal to lock stimulation to the animal's motion, with stable EMG signal over several months. To decrease assembly time, reduce assembly errors, and improve repeatability, we fabricate assembly aids including a drive holder, a drill guide, an implant fixture for microelectode “pinning”, and a gold plating fixture. Significance The expanding capability of optogenetic tools motivates continuing development of small optoelectric devices for stimulation and recording in freely moving mice. The OptoZIF Drive is the first to natively support ZIF-clip connection to recording hardware, which further supports a decrease in implant cross-section. The integrated 3D printed package of drive components and assembly tools facilities implant construction. The easy interfacing and installation of auxiliary electrodes makes the OptoZIF Drive especially attractive for real time feedback stimulation experiments.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

OptoZIF Drive: a 3D printed implant and assembly tool package for neural recording and optical stimulation in freely moving mice

et al. 2016

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“…We have previously described our custom hyperdrives for optogenetic stimulation and recording of SI [21], and 6th Annual International IEEE EMBS Conference on Neural Engineering San Diego, California, 6 -8 November, 2013 adaptation of EMG from rats [6], [22] to mice [23]. Briefly, in a single surgery, hyperdrives were implanted over left SI (3.75 mm lateral, 1.5 mm posterior from Bregma), and bilateral, bipolar EMG electrodes inserted under the mystacial pad to measure aggregate muscular activity.…”

Section: B Signal Acquisition and Processingmentioning

confidence: 99%

Stimulation of somatosensory cortex locked to whisker motions in a mouse model of active sensing

Schroeder

Mariano

Telian

et al. 2013

2013 6th International IEEE/EMBS Conference on Neural Engineering (NER)

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Active sensing incorporates closed-loop behavioral selection of information during sensory acquisition. The rodent whisker tactile system provides an ideal platform for studying these processes. We developed methods to deliver optogenetic feedback to mouse primary somatosensory cortex (SI), timelocked to active sensing motions (whisking) estimated through facial electromyography (EMG). To explore the impact of SI activity on individual whisker motions, we delivered stimulation locked to EMG threshold crossings at multiple delays. We found that stimulation regularized whisking (increasing overall periodicity), and shifted whisking frequency. These behavioral changes emulate changes observed when rodents actively contact objects, suggesting a role for SI in action selection that could guide design of sensory neuroprostheses sensitive to active sensing context.

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Selection of head and whisker coordination strategies during goal-oriented active touch

Schroeder

Ritt

2016

Journal of Neurophysiology

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In the rodent whisker system, a key model for neural processing and behavioral choices during active sensing, whisker motion is increasingly recognized as only part of a broader motor repertoire employed by rodents during active touch. In particular, recent studies suggest whisker and head motions are tightly coordinated. However, conditions governing the selection and temporal organization of such coordinated sensing strategies remain poorly understood. We videographically reconstructed head and whisker motions of freely moving mice searching for a randomly located rewarded aperture, focusing on trials in which animals appeared to rapidly "correct" their trajectory under tactile guidance. Mice orienting after unilateral contact repositioned their whiskers similarly to previously reported head-turning asymmetry. However, whisker repositioning preceded head turn onsets and was not bilaterally symmetric. Moreover, mice selectively employed a strategy we term contact maintenance, with whisking modulated to counteract head motion and facilitate repeated contacts on subsequent whisks. Significantly, contact maintenance was not observed following initial contact with an aperture boundary, when the mouse needed to make a large corrective head motion to the front of the aperture, but only following contact by the same whisker field with the opposite aperture boundary, when the mouse needed to precisely align its head with the reward spout. Together these results suggest that mice can select from a diverse range of sensing strategies incorporating both knowledge of the task and whisk-by-whisk sensory information and, moreover, suggest the existence of high level control (not solely reflexive) of sensing motions coordinated between multiple body parts.

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Extraction of intended palpation times from facial EMGs in a mouse model of active sensing

Cited by 3 publications

References 24 publications

OptoZIF Drive: a 3D printed implant and assembly tool package for neural recording and optical stimulation in freely moving mice

OptoZIF Drive: a 3D printed implant and assembly tool package for neural recording and optical stimulation in freely moving mice

Stimulation of somatosensory cortex locked to whisker motions in a mouse model of active sensing

Selection of head and whisker coordination strategies during goal-oriented active touch

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