Extraction of iron from plant leaves by Fe (II) chelators

Abadı́a, Javier; Monge, E.; Montañés, L.; Heras, L.

doi:10.1080/01904168409363241

Cited by 70 publications

(34 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar reduction of added Fe(III) was observed in the leaf extracts prepared by incubating the material in N HCl according to Takkar and Kaur (24) (Fig. 2C), or by incubating the material in 2,2'-bipyridyl, following the Abadia et al (1) procedure (Fig. 2D) …”

Section: Iron Reduction In Leaf Extractsmentioning

confidence: 70%

“…These results indicate the presence of strong reducing substances in the leaf extracts. We also measured the reduction of added Fe(III) using other methods for Fe(II) determination (1,9,24). Iron (100 Ag) added as Fe(III) at the onset of the incubation period was completely reduced in 1.5 h by g leafextracts in o-phe under both light and dark conditions ( Fig.…”

Section: Iron Reduction In Leaf Extractsmentioning

confidence: 99%

“…This fraction has been suggested to comprise Fe in the ferrous form (24). A number of methods using different extractants, chiefly weak acids or chelating agents, are now available for the determination of different fractions of Fe in plants (1,3,9,11,12,14,18,19,24).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Reduction of Iron by Leaf Extracts and Its Significance for the Assay of Fe(II) Iron in Plants

Mehrotra

Gupta²

1990

Plant Physiol.

View full text Add to dashboard Cite

"Active Fe" in plants has generally been interpreted to be ferrous iron. This study evaluated some of the commonly followed active Fe determination procedures based on Fe(ll) measurements. In each of 12 species examined, leaf extracts exhibited strong reducing activity and quickly reduced any added Fe(lIl) with or without light. The reducing activity was attributed to ascorbic acid and phenols in the plant extracts. The reliability of the Fe(ll) iron determination procedures in plants and the interpretation that active Fe is Fe(ll) are questionable. arietinum L.), citrus (Citrus aurantium L.), grape ( Vitis vinifera L.), maize (Zea mays L.), mulberry (Morus alba L.), radish (Raphanus sativus L.), rice (Oryza sativa L.), and ridge gourd (Luffa acutangula (L.) Roxb.) from 10 to 12 randomly selected field-grown plants were combined and cleaned against surface contamination by successive washings with 0.1 N HCI, tap water, deionized water, and finally glass distilled water. After blotting, the leaf samples were chopped finely (1-2 mm) using stainless steel scissors, and suitable quantities were drawn. The weighed samples were stored at 4°C and utilized for extraction within 1 h.

show abstract

Section: Iron Reduction In Leaf Extractsmentioning

confidence: 70%

Section: Iron Reduction In Leaf Extractsmentioning

confidence: 99%

See 1 more Smart Citation

Reduction of Iron by Leaf Extracts and Its Significance for the Assay of Fe(II) Iron in Plants

Mehrotra

Gupta²

1990

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…The iron was determined by atomic absorption spectrometry. The determination of the physiological iron was carried out following the method of Abadía et al (1984), using -'-dipyridyl.…”

Section: Methodsmentioning

confidence: 99%

Proteins, Photosynthetic Pigments and Polar Lipids Changes of Thylakoid Biomembrane From Prunus Affected by Iron Chlorosis

Pacheco¹,

García²,

Falcón³

1997

Acta Hortic.

Self Cite

View full text Add to dashboard Cite

The effect of iron chlorosis on proteins, photosynthetic pigments and acyl lipid composition of adult field grown peach tree leaves has been studied. No differences in the samples have been found between the iron extracted by ,´-dipyridyl (active iron) and total iron analysed by atomic absorption spectrometry, both analyses are good determinations to detect iron chlorosis. The levels of proteins, photosynthetic pigments and acyl lipids were reduced under iron chlorosis, although the concentrations of proteins and polar lipids per chlorophyll unit were higher in the chlorotic peach tree than in the control.

show abstract

“…-2,2' Bipyridyl solution (1.5% , pH 7.0) (2,2'Bip.) according to the EVALUATION OF DIFFERENT PLANT ACTIVE ... method described by Abadia et al (1984). -0.1N ethylendiamine tetraacetic acid pH 7.0 (EDTA) according to the method described by Mehrotra et al (1985).…”

Section: Active Ironmentioning

confidence: 99%

Evaluation of Different Plant Active Iron Extractants under Conditions of Egypt

2015

Egypt.J. Soil Sci.

View full text Add to dashboard Cite

Extraction of iron from plant leaves by Fe (II) chelators

Cited by 70 publications

References 10 publications

Reduction of Iron by Leaf Extracts and Its Significance for the Assay of Fe(II) Iron in Plants

Reduction of Iron by Leaf Extracts and Its Significance for the Assay of Fe(II) Iron in Plants

Proteins, Photosynthetic Pigments and Polar Lipids Changes of Thylakoid Biomembrane From Prunus Affected by Iron Chlorosis

Evaluation of Different Plant Active Iron Extractants under Conditions of Egypt

Contact Info

Product

Resources

About