Extraction of lipids from cottonseed tissue: III. Cyclopropenoid fatty acids from glanded and glandless seed

Jacks, T. J.; Hensarling, T. P.; Yatsu, L. Y.; Brown, L. E.

doi:10.1007/bf02671105

J Americ Oil Chem Soc

1973

DOI: 10.1007/bf02671105

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Extraction of lipids from cottonseed tissue: III. Cyclopropenoid fatty acids from glanded and glandless seed

T. J. Jacks

T. P. Hensarling

L. Y. Yatsu

et al.

Abstract: Principal storage sites of cyclopropenoid fatty acids in glanded and glandless cottonseed tissues were investigated by measuring the content of cyclopropenoid fatty acids in lipids obtained by a cytoplasmic disruptive solvent (hexane-acetone-water) and a non-

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Cited by 2 publications

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Electron microscopic cytochemistry of cyclopropenoids in cottonseed (Gossypium hirsutum L.) tissues

Yatsu¹,

Kircher²

1987

Lipids

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An electron microscopic cytochemical procedure for locating cyclopropenoids (CYP) in oilseeds is proposed. The procedure is based on the fact that metallic silver is produced when AgNO3 reacts with CYP. Since so many other compounds react with AgNO3, we treated specimens that do and not contain CYP with AgNO3 and looked for electron-dense stains that were unique to CYP(+) seeds. Virtually everything in all seed specimens, regardless of the presence or absence of CYP, was stained. However, cottonseeds contained two additional staining constituents not found in controls: (i) certain large, intracellular droplets, and (ii) the cuticles, both occurring exclusively in the axial organs. Extraction of the speciments with ether abolished the stainability of the droplets but did not affect cuticles. Arguments for the validity of the test are presented.

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Electron microscopic cytochemistry of cyclopropenoids in cottonseed (Gossypium hirsutum L.) tissues

Yatsu¹,

Kircher²

1987

Lipids

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Lipids in foods: Problems and procedures in collating data

Kinsella

Posati

Weihrauch

et al. 1975

C R C Critical Reviews in Food Technology

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Extraction of lipids from cottonseed tissue: III. Cyclopropenoid fatty acids from glanded and glandless seed

Abstract: Principal storage sites of cyclopropenoid fatty acids in glanded and glandless cottonseed tissues were investigated by measuring the content of cyclopropenoid fatty acids in lipids obtained by a cytoplasmic disruptive solvent (hexane-acetone-water) and a non-

Cited by 2 publications

References 6 publications

Electron microscopic cytochemistry of cyclopropenoids in cottonseed (Gossypium hirsutum L.) tissues

Electron microscopic cytochemistry of cyclopropenoids in cottonseed (Gossypium hirsutum L.) tissues

Lipids in foods: Problems and procedures in collating data

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