Extraction of Mastitis Pathogen DNA from Sample Collecting Cards: Practical Consequences

Durel, Luc; Benoit, François; Treilles, Michaël; Farre, Michael

doi:10.15744/2348-9790.1.602

Journal of Veterinary Science and Animal Husbandry

2015

DOI: 10.15744/2348-9790.1.602

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Extraction of Mastitis Pathogen DNA from Sample Collecting Cards: Practical Consequences

Luc Durel¹,

François Benoit²,

Michaël Treilles³

et al.

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Cited by 2 publications

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“…FTA cards have been previously used for the detection of other bacteria in BTM samples (Durel et al, 2015;Azevedo et al, 2016) and with other matrices, such as blood, for detection of C. burnetii (Pascucci et al, 2015) in wild animals. To our knowledge, FTA cards have never been used for C. burnetii DNA detection from BTM.…”

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confidence: 99%

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QTest: A new way to easily sample, store, and ship samples to perform Q fever PCR analysis on bulk tank milk

Treilles¹,

Charollais²,

Guatteo

et al. 2021

JDS Communications

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In dairy cattle at the herd level, Coxiella burnetii can be diagnosed by PCR on bulk tank milk (BTM). To facilitate the collection and shipment of BTM samples to the laboratory, we validated the use of Whatman FTA Elute Micro Cards for the conservation of BTM in 2 studies. The first study evaluated the stability of samples over time and at 2 different storage temperatures. The second compared results for BTM stored on FTA cards and raw BTM stored as is.

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mentioning

confidence: 99%

“…This option can prevent the problems encountered under field conditions; indeed, FTA cards are already used for the diagnosis of pathogens responsible for mastitis in milk, including Staphylococcus aureus, Streptococcus agalactiae, and Mycoplasma spp. (Durel et al, 2015;Tardy et al, 2019).…”

mentioning

confidence: 99%

QTest: A new way to easily sample, store, and ship samples to perform Q fever PCR analysis on bulk tank milk

Treilles¹,

Charollais²,

Guatteo

et al. 2021

JDS Communications

View full text Add to dashboard Cite

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Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

2022

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Dried Blood Spots (DBS) technology has become a valuable tool in medical studies, however, in veterinary and biological research DBS technology applications are still limited. Up-to-date no review has comprehensively integrated all the evidence existing across the fields, technologies and animal species. In this paper we summarize the current applications of DBS technology in the mentioned areas, and provide a scope of different types of dried sample carriers (cellulose and non-cellulose), sampling devices, applicable methods for analyte extraction and detection. Mammals, birds, insects and other species are represented as the study objects. Besides the blood, the review considers a variety of specimens, such as milk, saliva, tissue samples and others. The main applications of dried samples highlighted in the review include epidemiological surveys and monitoring for infections agents or specific antibodies for disease/vaccination control in households and wildlife. Besides the genetic investigations, the paper describes detection of environmental contaminants, pregnancy diagnosis and many other useful applications of animal dried samples. The paper also analyses dried sample stability and storage conditions for antibodies, viruses and other substances. Finally, recent developments and future research for DBS technology in veterinary medicine and biological sciences are discussed.

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Extraction of Mastitis Pathogen DNA from Sample Collecting Cards: Practical Consequences

Cited by 2 publications

References 18 publications

QTest: A new way to easily sample, store, and ship samples to perform Q fever PCR analysis on bulk tank milk

QTest: A new way to easily sample, store, and ship samples to perform Q fever PCR analysis on bulk tank milk

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

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