Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization

Onoja, Emmanuel; Chandren, Sheela; Razak, Fazira Ilyana Abdul; Wahab, Roswanira Abdul

doi:10.1016/j.jbiotec.2018.07.036

Cited by 40 publications

(32 citation statements)

References 87 publications

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“…The support was prepared by dispersing freshly prepared magnetite (1.0 g) [3] in deionized water (5.0 mL), acidified with 6.0 M HCl (1 mL) and sonicated for 10 min. In a separate plastic vial, 6.0 M HCl solution (1.0 mL) was added to the sodium silicate solution (25.0 mL) to lower the pH of the solution to pH 12.0.…”

Section: Preparation Of Supportmentioning

confidence: 99%

“…The reasons behind this have to do with its well-reported good stereospecificity, high enantioselectivity, and stability in organic solvents, as well as possessing good thermal stability, the capability of catalyzing a plethora of important industrial reactions. Among these reactions, CRL catalyzes several reactions including hydrolysis, transesterification, esterification, and interesterification which require mild optimal working conditions through an energy-saving route [1][2][3][4]. However, like any other forms of free enzymes, the free form of CRL is vulnerable to deactivation under harsh industrial settings [5].…”

Section: ■ Introductionmentioning

confidence: 99%

“…Resorting to using renewable and cost-effective natural polymers, for instance, rice husk [6], chitosan [7], bamboo [8] and OPL [3] to prepare matrices for biocatalysts development, is in fact, a greener technique for this purpose. Numerous studies exploring the different plant-based matrices have been reported.…”

Section: ■ Introductionmentioning

confidence: 99%

“…A versatile crosslinker, glutaraldehyde [22] was chosen to covalently bind the primary amino groups of CRL to the surface of OPL-silica support matrix (CRL/G/A/SiO2-M). This homobifunctional crosslinker activates the functional groups of different support matrices by linking the primary amino groups on the support surface with those of the protein molecules to be immobilized [3,4,[22][23][24].…”

Section: ■ Introductionmentioning

confidence: 99%

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Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Onoja

Wahab

2019

Indones. J. Chem.

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Till date, studies that investigated the effect of glutaraldehyde concentration on catalytic efficacy of biocatalyst developed with silica-derived from oil palm leaves (OPL) as support, are unknown. The study presents the preparation of a support consisting of silica extracted from OPL coated over magnetite (G/A/SiO2-M) for the immobilization of Candida rugosa lipase (CRL). Herein, the effect of glutaraldehyde concentration on the catalytic efficacy of immobilized CRL was assessed by the enzymatic production of butyl butyrate as a model. Fourier transform infrared (FTIR) spectra and immobilization parameters indicated that covalent bound CRL on functionalized OPL-derived silica-magnetite composite activated with 4% (v/v) glutaraldehyde solution (100 mM, pH 7.0) (CRL/G/A/SiO2-M) and pretreated in toluene, resulted in a protein loading and an immobilization yield of 68.3 mg/g and 74.3%, respectively. The resultant CRL/G/A/SiO2-M biocatalyst which specific activity was 61.9 U/g catalyzed the esterification production of 76.5% butyl butyrate in just 3 h, as confirmed by analyses of the purified ester using FTIR and 1H NMR spectroscopy. Hence, the finding envisages the promising use of G/A/SiO2-M support fabricated from discarded OPL as a carrier for immobilization and activation of CRL, in conjunction to being a good alternative source of renewable silica.

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Section: Preparation Of Supportmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Glutaraldehyde Concentration on Catalytic Efficacy of <i>Candida rugosa</i> Lipase Immobilized onto Silica from Oil Palm Leaves

Onoja

Wahab

2019

Indones. J. Chem.

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“…Biomass-derived from both whether living or non-living organisms, as well as can equally apply to both animal and vegetable derived materials. Others have described that the term 'biomass' is related to the organically based waste matter of plant and animal origins [1,2], in which sewage and municipal solid wastes are also found in this category [3]. Whereas Kwietniewska and Tys [4] described biomass rather differently, namely as the biodegradable fraction of agricultural products, wastes, and residues, including those of vegetable and animal substances, alongside wastes from forestry and related industries.…”

Section: Introductionmentioning

confidence: 99%

On the Renewable Polymers from Agro-industrial Biomass: A Mini Review

Wahab

Gowon²,

Elias

2019

J. Idn. Chem. Soc.

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Plant biomass is the most abundant natural resources on earth. However, current strategies for the utilization of agricultural biomass is far from efficient, thus environmental issues related to incompetent management of biomass prevail. Innovative handling of surplus biomass can yield several rewards, which includes renewability and sustainability of the commodity as feedstock for industrial and energy purposes. In fact, an array of different parts of a plant or agro-industrial biomass, for instance, shell, husks, wood, and leaves were successfully converted into advanced carbon materials, for use as absorbent, catalyst enzyme support, electrode, etc. In this review, an extensive literature survey related to areas of renewable sources of biopolymer in both the agricultural and industrial sectors were performed. Information on their industrial value and uses, the fundamentals of their extraction alongside the benefits and major drawbacks of their utilization, are also highlighted. We aim to show that the smart utilization of unwanted agro-industrial biomass encompasses a portion of a bigger scheme that intelligently uses biomass to complement current agricultural advancements that create smart crops and growing them using cleverly designed technology. The best part of this “Waste to Wealth” concept is that every part of the crop is fully utilized. However, a set of clear criteria must be in place to ensure a sustained momentum, so that the green approach of responsible biomass utilization will be fully embraced by nations worldwide.

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