Extraction of natural hydroxyapatite from tilapia fish scales using alkaline treatment

Zainol, Ismail; Adenan, Nur Hamiza; Rahim, N A; Jaafar, Che Nor Aiza

doi:10.1016/j.matpr.2019.06.072

Cited by 26 publications

(18 citation statements)

References 14 publications

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“…The SEM micrographs of HAp extracted from fish scales are shown in Figure 4. It can be seen in Figure 4A that the material consists exclusively of particles, since the presence of collagen fibers was not visualized as reported by Zainol et al 27 This result reinforces the efficiency of alkaline treatment in removing organic matter. The biomaterial is composed of agglomerated particles without a defined morphology.…”

Section: Resultssupporting

confidence: 84%

In vitro evaluation of natural hydroxyapatite from Osteoglossum bicirrhosum fish scales for biomedical application

Horta

Moura

Aguilar

et al. 2021

Int J Applied Ceramic Tech

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This study reports the obtainment of bioactive hydroxyapatite (HAp) extracted from scales of arowana fish (FSHA) (Osteoglossum bicirrhosum) by alkaline treatment followed by calcination at 600 and 800°C. The cell viability and bioactivity of hydroxyapatite particles (FSHA) were investigated and compared with those of HAp synthesized (s.HA) by the precipitation method. The HAp particles from fish scales showed non‐toxic behavior to dental pulp stem cells similar to HAp synthesized. Additionally, bioactivity assays show that the Hap from natural source forms the bone‐like apatite layer faster than s.HA sample, after being incubated in McCoy medium for 3 days. The results illustrate that HAp obtained from Osteoglossum bicirrhosum fish scale bio‐waste showed excellent biocompatibility. Besides, this study provides an effective method for converting low‐cost bio‐waste into a value‐added and it can be a potential alternative biomaterial for various biomedical applications.

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Section: Resultssupporting

confidence: 84%

In vitro evaluation of natural hydroxyapatite from Osteoglossum bicirrhosum fish scales for biomedical application

Horta

Moura

Aguilar

et al. 2021

Int J Applied Ceramic Tech

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show abstract

“…The isolated reflection at  of .5°, corresponding to the (002) Miller plan, was chosen for the calculation leading to the crystallite size of 10.5 ± 1 nm. This result agrees with the size measured at 12 nm for fish scale of Catla catla after calcination at 200°C [50] or with the size of 20-30 nm which was measured by SEM with transmission mode for hydroxyapatite particles of tilapia fish scales [51]. 11.20, the uptake decreased from its greatest value of 34.26 mg g -1 to 0.59 mg g -1 .…”

Section: Characterization Of Fs Biosorbentsupporting

confidence: 90%

Fish scale of Sardina pilchardus as a biosorbent for the removal of Ponceau S dye from water: Experimental, designing and Monte Carlo investigations

Jaafar

Darchen²,

Driouich³

et al. 2022

Inorganic Chemistry Communications

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“…In the present study, we isolated and characterized MC and CHA from P. olivaceus using pepsin hydrolysis and alkaline hydrolysis, respectively [34]. Numerous studies have reported about HA and collagen extracted and isolated from different fish species, such as Oncorhynchus keta, Thunnus obesus, and Oreochromis [35][36][37]. Unlike thermally extracted HA, HA extracted through alkaline hydrolysis retains its carbonate groups, because carbonate cannot be separated without heat treatment [36].…”

Section: Discussionmentioning

confidence: 99%

A 3D-Printed Polycaprolactone/Marine Collagen Scaffold Reinforced with Carbonated Hydroxyapatite from Fish Bones for Bone Regeneration

Kim

Heo

et al. 2022

Marine Drugs

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In bone tissue regeneration, extracellular matrix (ECM) and bioceramics are important factors, because of their osteogenic potential and cell–matrix interactions. Surface modifications with hydrophilic material including proteins show significant potential in tissue engineering applications, because scaffolds are generally fabricated using synthetic polymers and bioceramics. In the present study, carbonated hydroxyapatite (CHA) and marine atelocollagen (MC) were extracted from the bones and skins, respectively, of Paralichthys olivaceus. The extracted CHA was characterized using Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analysis, while MC was characterized using FTIR spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The scaffolds consisting of polycaprolactone (PCL), and different compositions of CHA (2.5%, 5%, and 10%) were fabricated using a three-axis plotting system and coated with 2% MC. Then, the MC3T3-E1 cells were seeded on the scaffolds to evaluate the osteogenic differentiation in vitro, and in vivo calvarial implantation of the scaffolds was performed to study bone tissue regeneration. The results of mineralization confirmed that the MC/PCL, 2.5% CHA/MC/PCL, 5% CHA/MC/PCL, and 10% CHA/MC/PCL scaffolds increased osteogenic differentiation by 302%, 858%, 970%, and 1044%, respectively, compared with pure PCL scaffolds. Consequently, these results suggest that CHA and MC obtained from byproducts of P. olivaceus are superior alternatives for land animal-derived substances.

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Extraction of natural hydroxyapatite from tilapia fish scales using alkaline treatment

Cited by 26 publications

References 14 publications

In vitro evaluation of natural hydroxyapatite from Osteoglossum bicirrhosum fish scales for biomedical application

In vitro evaluation of natural hydroxyapatite from Osteoglossum bicirrhosum fish scales for biomedical application

Fish scale of Sardina pilchardus as a biosorbent for the removal of Ponceau S dye from water: Experimental, designing and Monte Carlo investigations

A 3D-Printed Polycaprolactone/Marine Collagen Scaffold Reinforced with Carbonated Hydroxyapatite from Fish Bones for Bone Regeneration

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