Extraction of nucleic acids from blood: unveiling the potential of active pneumatic pumping in centrifugal microfluidics for integration and automation of sample preparation processes

Brassard, D.; Geißler, Matthias; Descarreaux, Marianne; Tremblay, Dominic; Daoud, Jamal; Clime, Liviu; Mounier, Maxence; Charlebois, D.; Veres, Teodor

doi:10.1039/c9lc00276f

Cited by 68 publications

(60 citation statements)

References 36 publications

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“…(1) mechanical-by grinding, freeze-thawing, potterization, centrifugation, sonication or bead-beating; (2) chemical-by detergents, solubilizing membrane lipids, or by chaotropic agents, perforating cell membranes with the denaturation of trans-membrane proteins, by Both types, mostly based on a magnetic beads extraction or, less commonly, on silica filtration, are developed onto a rotating platform where sample and reagents motion is enabled by centrifugal and related forces (Coriolis and Euler) that, usually in combination with pneumatic forces, handle the fluidic system and enable sample processing. This principle is applied in traditional rectangular LOCs [36,71,86] and in LOD systems [18,41,51,53,54,60]. These devices generally manage the sample by changing speed and sense of rotation: a particular example of a LOD is represented by LabDisk analyzer [28,64] which implements an automated lyse-bind-wash-elute protocol by means of a gas-phase transition magnetophoresis principle, which combines magnetic and centrifugal forces to carry NA-complexed magnetic beads through the separation liquid/gas interfaces between each reaction chamber.…”

Section: Raw Samples Pre-treatmentmentioning

confidence: 99%

“…Different silica structures have been implemented in LOCs for NA extraction (Figure 5). As an interesting example, Brassard et al [36] utilize a double layer of glass microfiber filters (composed of silica with sodium, potassium or calcium) where the first layer (2.7 µm. pored) acts as a pre-filter for whole blood, while the second layer (0.7 µm.…”

Section: Solid-phase Extractionmentioning

confidence: 99%

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An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples

Obino

Vassalli

Franceschi

et al. 2021

Sensors

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Nucleic acid (NA) extraction is a basic step for genetic analysis, from scientific research to diagnostic and forensic applications. It aims at preparing samples for its application with biomolecular technologies such as isothermal and non-isothermal amplification, hybridization, electrophoresis, Sanger sequencing and next-generation sequencing. Multiple steps are involved in NA collection from raw samples, including cell separation from the rest of the specimen, cell lysis, NA isolation and release. Typically, this process needs molecular biology facilities, specialized instrumentation and labor-intensive operations. Microfluidic devices have been developed to analyze NA samples with high efficacy and sensitivity. In this context, the integration within the chip of the sample preparation phase is crucial to leverage the promise of portable, fast, user-friendly and economic point-of-care solutions. This review presents an overview of existing lab-on-a-chip (LOC) solutions designed to provide automated NA extraction from human raw biological fluids, such as whole blood, excreta (urine and feces), saliva. It mainly focuses on LOC implementation aspects, aiming to describe a detailed panorama of strategies implemented for different human raw sample preparations.

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Section: Raw Samples Pre-treatmentmentioning

confidence: 99%

Section: Solid-phase Extractionmentioning

confidence: 99%

An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples

Obino

Vassalli

Franceschi

et al. 2021

Sensors

View full text Add to dashboard Cite

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“…To this end, the bioanalytical protocols are dissected into laboratory unit operations (LUOs), each of them controlled by normally closed valve. Centrifugally implemented LUOs have been covered extensively in the literature, e.g., for metering / aliquoting [45][46][47], mixing [48][49][50][51], incubation, purification / concentration / extraction [35,52], homogenisation [53,54], particle filtering [55][56][57][58][59] and droplet generation [60][61][62].…”

Section: Laboratory Unit Operationsmentioning

confidence: 99%

“…By virtue of the ubiquitous and unidirectional centrifugal field acting simultaneously on all liquids loaded to their potentially fast spinning device, valving concepts play a prominent role in LoaD technologies. While there have been active modes of control, e.g., by co-rotating pumps [34,35] or sacrificial barriers [36,37] opened by stimuli delivered by instrument mounted units, rotationally actuated schemes have attracted particular attention due to their smooth alignment with the simple and robust concept of LoaD platform with a rugged instrument "playing" a mostly single-use disc cartridge.…”

Section: Introductionmentioning

confidence: 99%

Design Optimization of Centrifugal Microfluidic "Lab-on-a-Disc" Systems towards Fluidic Larger-Scale Integration

Ducrée¹

2021

Preprint

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Enhancing the degree of functional multiplexing while assuring operational reliability and manufacturability at competitive costs are crucial components to enable comprehensive sample-to-answer automation, e.g., for use in common, decentralized “Point-of-Care” or “Point-of-Use” scenarios. This paper demonstrates a model-based ‘digital twin’ approach which efficiently supports the algorithmic design optimization of exemplary centrifugo-pneumatic (CP) dissolvable-film (DF) siphon valves towards larger-scale integration (LSI) of well-established “Lab-on-a-Disc” (LoaD) systems. Obviously, the spatial footprint of the valves and their upstream laboratory unit operations (LUOs) have to fit, at a given radial position prescribed by its occurrence in the assay protocol, into the locally available disc space. At the same time, the retention rate of rotationally actuated valve and, most challenging, its band width related to unavoidable experimental tolerances need to slot into a defined interval of the practically allowed frequency envelope. A set of design rules, metrics, and methods and instructive showcases for computationally assisted optimization of valve structures are presented.

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“…Many of these methods are summarized in ►Fig. 3(C), and include capillary forces, 171 vacuum driven flow, 146 gravity driven flow, 199 mechanical peristaltic pumps, 200 centrifugation, 201 surface acoustic wave actuation, 202 manual finger-press actuation, 203 and pneumatic peristaltic pumps. 204,205 While numerous on-chip blood actuation methods are outlined in the literature, generally very little consideration is given to material hemocompatibility, or functional effects of the manipulation mechanisms regarding operational hemocompatibility, factors that may severely hamper application of on-chip blood handling systems.…”

Section: Microfluidic Technologies For On-chip Blood Manipulationmentioning

confidence: 99%

A Review of Design Considerations for Hemocompatibility within Microfluidic Systems

Szydzik

Brazilek

Nesbitt

2020

Semin Thromb Hemost

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The manipulation of blood within in vitro environments presents a persistent challenge, due to the highly reactive nature of blood, and its multifaceted response to material contact, changes in environmental conditions, and stimulation during handling. Microfluidic Lab-on-Chip systems offer the promise of robust point-of-care diagnostic tools and sophisticated research platforms. The capacity for precise control of environmental and experimental conditions afforded by microfluidic technologies presents unique opportunities that are particularly relevant to research and clinical applications requiring the controlled manipulation of blood. A critical bottleneck impeding the translation of existing Lab-on-Chip technology from laboratory bench to the clinic is the ability to reliably handle relatively small blood samples without negatively impacting blood composition or function. This review explores design considerations critical to the development of microfluidic systems intended for use with whole blood from an engineering perspective. Material hemocompatibility is briefly explored, encompassing common microfluidic device materials, as well as surface modification strategies intended to improve hemocompatibility. Operational hemocompatibility, including shear-induced effects, temperature dependence, and gas interactions are explored, microfluidic sample preparation methodologies are introduced, as well as current techniques for on-chip manipulation of the whole blood. Finally, methods of assessing hemocompatibility are briefly introduced, with an emphasis on primary hemostasis and platelet function.

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Extraction of nucleic acids from blood: unveiling the potential of active pneumatic pumping in centrifugal microfluidics for integration and automation of sample preparation processes

Abstract: NAs are extracted from blood using a pneumatic centrifugal platform.

Cited by 68 publications

References 36 publications

An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples

An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples

Design Optimization of Centrifugal Microfluidic "Lab-on-a-Disc" Systems towards Fluidic Larger-Scale Integration

A Review of Design Considerations for Hemocompatibility within Microfluidic Systems

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