Extraction of Oleic Acid from Animals Oil and Its Anti-inflammatory Effect on Network Pharmacology

Abstract

The purpose of this paper is to explore the possible mechanisms of anti-inflammatory and scar repair by Mongolian horse oil. We used TCM database and literature mining to collect active compounds of horse oil and used Swiss TargetPrediction and SuperPred server to find targets of compounds. Anti-inflammatory drug targets were collected through the CTD database. Go annotation of targets and KEGG pathway were enriched and analyzed through Metascape database platform. Molecular docking between active ingredients and targets was verified by AutoDock software. Metascape analysis revealed that the key candidate targets were significantly enriched in a number of pathways associated with inflammatory pathology. The results of molecular docking showed that oleic acid, a major component of animals oil, could influence the regulatory functions of TNF, NGF, IL6, IL1B, Jun, and CDK1. This suggests that animals oil can regulate the development of inflammation through its active ingredient, oleic acid, and can influence the expression of multiple signaling pathways, with theoretical endogenous interactions with TNF, NGF, IL6, IL1B, JUN, and CDK1 proteins.

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“…GO annotation analysis and KEGG pathway analysis were conducted on the key targets by using the Metascape platform ( Yu et al, 2021 ). The results are shown in Figure 7 .…”

“…The key targets were put into the Metascape database, and the species were set as “ Homo sapiens ,” with p < 0.01 from the results of PPI analysis. GO annotations of targets and KEGG pathways were enriched and analyzed through the Metascape ( http://metascape.org ) database platform, which could analyze the biological processes and pathways of genes ( Yu et al, 2021 ). In addition, the correspondence was established among active compounds, key targets, and the top 20 pathways of the KEGG pathway enrichment analysis results and visualized by Cytoscape 3.8.2 software.…”