Extraction of p-coumaric acid from agricultural residues and separation using ‘sugaring out’

Dhamole, Pradip B.; Chavan, S. M.; Patil, Pallavi S.; Feng, Hao; Bule, Mahesh V.; Kinninge, Pallavi

doi:10.1007/s11814-016-0020-y

Cited by 24 publications

(10 citation statements)

References 27 publications

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“…Sucrose contains one glucose and one fructose molecule compared to two glucose molecules of maltose. They each can form eight hydrogen bonds whereas glucose and xylose can form only five and four bonds, respectively . However, the difference in number of moles of sucrose (0.876 moles) and maltose (0.876 moles) for the same mass of glucose (1.665 moles) at 300 g/L limits the efficiency of two phase formation .…”

Section: Results and Discussion For Optimization Of Lbef Systemmentioning

confidence: 99%

“…As the sugar concentration of bottom phase increases, there are lesser hydrogen bonds available for the released proteins to attach, they rise to the top phase and can be extracted. The increase in volume of ACN with higher glucose concentration also enhances extraction of non‐polar proteins as ACN is hydrophobic (non‐polar) compared to water . Statistical analysis of this study suggested that the data obtained is significant (p < 0.05) and 300 g/L of sugar concentration was selected for optimization of further parameters.…”

Section: Results and Discussion For Optimization Of Lbef Systemmentioning

confidence: 99%

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Optimization of protein extraction from Chlorella Vulgaris via novel sugaring‐out assisted liquid biphasic electric flotation system

Koyande

Chew

Lim

et al. 2019

Engineering in Life Sciences

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Microalgae biomass has been consumed as animal feed, fish feed and in human diet due to its high nutritional value. In this experiment, microalgae specie of Chlorella Vulgaris FSP‐E was utilized for protein extraction via simple sugaring‐out assisted liquid biphasic electric flotation system. The external electric force provided to the two‐phase system assists in disruption of rigid microalgae cell wall and releases the contents of microalgae cell. This experiment manipulates various parameters to optimize the set‐up. The liquid biphasic electric flotation set‐up is compared with a control liquid biphasic flotation experiment without the electric field supply. The optimized separation efficiency of the liquid biphasic electric flotation system was 73.999 ± 0.739% and protein recovery of 69.665 ± 0.862% compared with liquid biphasic flotation, the separation efficiency was 61.584 ± 0.360% and protein recovery was 48.779 ± 0.480%. The separation efficiency and protein recovery for 5 × time scaled‐up system was observed at 52.871 ± 1.236% and 73.294 ± 0.701%. The integration of simultaneous cell‐disruption and protein extraction ensures high yield of protein from microalgae. This integrated method for protein extraction from microalgae demonstrated its potential and further research can lead this technology to commercialization.

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Section: Results and Discussion For Optimization Of Lbef Systemmentioning

confidence: 99%

Section: Results and Discussion For Optimization Of Lbef Systemmentioning

confidence: 99%

Optimization of protein extraction from Chlorella Vulgaris via novel sugaring‐out assisted liquid biphasic electric flotation system

Koyande

Chew

Lim

et al. 2019

Engineering in Life Sciences

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“…Other potential scalable approaches have been described, such as enzymatic [107][108][109][110][111][112][113][114], alkaline [115][116][117][118][119][120] and based on different types of aqueous media (e.g., cyclodextrins, montmorillonite K-10/LiOH, green liquor) [121][122][123][124][125][126][127][128][129][130]; ionic liquids [131][132][133][134][135], deep eutectic solvents [136][137][138], constituting alternative methods for the recovery of high added-value compounds from agro-industrial waste aiming at obtaining the best analytical, economical and socio-environmental compromise [139][140][141][142].…”

Section: From Conventional Solvent Separation To Enhancement Processimentioning

confidence: 99%

Green and Sustainable Separation of Natural Products from Agro-Industrial Waste: Challenges, Potentialities, and Perspectives on Emerging Approaches

Zuin

Ramin

2018

Topics in Current Chemistry Collections

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New generations of biorefinery combine innovative biomass waste resources from different origins, chemical extraction and/or synthesis of biomaterials, biofuels, and bioenergy via green and sustainable processes. From the very beginning, identifying and evaluating all potentially high value-added chemicals that could be removed from available renewable feedstocks requires robust, efficient, selective, reproducible, and benign analytical approaches. With this in mind, green and sustainable separation of natural products from agro-industrial waste is clearly attractive considering both socio-environmental and economic aspects. In this paper, the concepts of green and sustainable separation of natural products will be discussed, highlighting the main studies conducted on this topic over the last 10 years. The principal analytical techniques (such as solvent, microwave, ultrasound, and supercritical treatments), by-products (e.g., citrus, coffee, corn, and sugarcane waste) and target compounds (polyphenols, proteins, essential oils, etc.) will be presented, including the emerging green and sustainable separation approaches towards bioeconomy and circular economy contexts.

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“…Detailed phase separation conditions for acetonitrile, water, and glucose system were studied [24]. In the last few years sugaring-out has been extensively used for separation of various products which include 2,3 butanediol [25], lactic acid [26], acetoin [27], succinic acid [28,29], antibiotics [30,31], proteins [32], phenolics [33,34] and even in analysis of some compounds [35,36]. Glucose results in better separation and hence is used in most of the studies on sugaring out extraction [22][23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

“…1) lation and the solute was crystallized [29]. Besides, sugaring-out has several other advantages, such as rapid phase separation, works over a wide temperature range (1-30 o C) without changing environment conditions (e.g., pH), and minimizing the corrosion problem [24,34].…”

Section: Introductionmentioning

confidence: 99%

Sugaring-out extraction of erythromycin from fermentation broth

Moharkar

Dhamole

2021

Korean J. Chem. Eng.

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This study reports the sugaring-out extraction of erythromycin from fermentation broth using acetonitrile (ACN) as solvent and glucose as a mass separating agent. Different process parameters--glucose concentration, temperature, ACN/water ratio and pH--were optimized to achieve maximum extraction of erythromycin. 88% (w/w) of erythromycin was extracted from the model system with following optimized conditions: glucose 156.3 g/L; temperature 4 o C; ACN/water ratio 1 and pH 8.3. Further, the effect of typical fermentation media components (starch, soybean flour, CaCO 3 , NaCl and (NH 4 ) 2 SO 4 ) on sugaring out extraction of erythromycin was also investigated. Starch, soybean flour and CaCO 3 were observed to affect erythromycin extraction only at higher concentration. Removal of suspended solids from simulated as well as real broth prior to extraction enhanced the extraction efficiency (from 72% to 87%). Sugaring out extraction of erythromycin was found to be more effective than salting out extraction. Also, higher partition coefficient was achieved in the present work than other reported methods using carbohydrates as mass separating agent. Further, it was found that the antimicrobial activity of erythromycin was preserved during sugaring out extraction of erythromycin.

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Extraction of p-coumaric acid from agricultural residues and separation using ‘sugaring out’

Cited by 24 publications

References 27 publications

Optimization of protein extraction from Chlorella Vulgaris via novel sugaring‐out assisted liquid biphasic electric flotation system

Optimization of protein extraction from Chlorella Vulgaris via novel sugaring‐out assisted liquid biphasic electric flotation system

Green and Sustainable Separation of Natural Products from Agro-Industrial Waste: Challenges, Potentialities, and Perspectives on Emerging Approaches

Sugaring-out extraction of erythromycin from fermentation broth

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