Extraction of pectin from sugar beet pulp by enzymatic and ultrasound-assisted treatments

Effect of pectin extraction method on properties of cellulose nanofibers isolated from sugar beet pulp

“…Extraction of pectin by enzymatic hydrolysis Extraction of pectin from SBP using cellulase and xylanase mixture was carried out as previously published by Abou-Elseoud et al (2021). A mixture of xylanase and cellulase in a ratio of 1:1.5 was prepared in citrate buffer at 25°C.…”

“…After 2 h, the precipitated pectin was centrifuged at 10,000 rpm for 20 min, washed with 70% ethanol, centrifuged again, and dried at 40°C for 48 h. The pectin yield was calculated as follows Eq. 2: Pectin yield ð%Þ ¼ ðdry weight of pectin/dry weight of SBPÞ Â 100: ð2Þ Extraction of pectin by acid hydrolysis was carried out as previously published by Abou-Elseoud et al (2021). SBP was suspended in water at a liquor ratio of 1:15 and acidified to pH 1 with sulfuric acid.…”

“…Pectin extraction from the cell wall of sugar beet requires breaking the bonds between cellulose, hemicelluloses, and pectin. Although hydrolysis using mineral acids is a common industrial method for pectin extraction, other methods, such as enzymatic hydrolysis, have also been studied (Zykwinska et al 2008;Concha et al 2013;Babbar et al 2016;Pacheco et al 2019;Abou-Elseoud et al 2021). In addition to being a more environmentally sound approach than mineral acid hydrolysis, enzymatic hydrolysis was also found to be effective in terms of pectin yield in these previous studies.…”

“…In addition, because different methods can be used for pectin extraction, the effect of these methods on the properties of isolated CNFs needs to be studied. The different methods of pectin extraction of course affect its properties; for example, pectin isolated from sugar beet pulp using cellulase/xylanase enzymes mixture was found to have lower galacturonic acid than that produced using sulfuric acid hydrolysis but pectin isolated by the enzymes mixture was higher in neutral sugars content, degree of esterification, and molecular weight (Abou-Elseoud et al 2021).…”

Effect of pectin extraction method on properties of cellulose nanofibers isolated from sugar beet pulp

“…Pectin extraction from the cell wall of sugar beet requires breaking the bonds between cellulose, hemicellulases, and pectin. Although hydrolysis using mineral acids is a common industrial method for pectin extraction, other methods, such as enzymatic hydrolysis, have also been studied (Zykwinska et al 2008;Concha et al 2013;Babbar et al 2016;Pacheco et al 2019;Abou-Elseoud et al 2021). In addition to being a more environmentally sound approach than mineral acid hydrolysis, enzymatic hydrolysis was also found to be effective in terms of pectin yield in these previous studies.…”