Extraction of phenolic compounds from fresh and wilt kesum plant using liquid biphasic flotation

Chia, Shir Reen; Foo, Shan Ping; Hew, Yoong Shern; Loh, Yuh Juann; Devadas, Vishno Vardhan; Chew, Kit Wayne; Show, Pau Loke

doi:10.1016/j.seppur.2020.116831

Cited by 16 publications

(5 citation statements)

References 22 publications

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“…Several authors reported different extraction methods of bioactives from tobacco and its waste [ 5 , 7 , 17 , 29 , 30 , 31 , 32 , 33 ]. However, no information could be found concerning the chemical composition of phenolic compounds, flavonoids, sugars, and alkaloids of tobacco leaf waste extracts; using non-conventional solvents such as NaDES does not appear to have been in the scope of any researcher.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the NaDES showed both chemical and thermal stability; these properties are associated with the stability of the extracted bioactive molecules during storage for their subsequent incorporation into a pharmaceutical and/or cosmetic product. Moreover, different solvents/biomass ratios, different extraction conditions, and extraction methodologies could be used to obtain biomolecules, i.e., maceration, liquid biphasic flotation, ultrasound, supercritical fluids, microwave-assisted extraction [ 5 , 7 , 17 , 29 , 30 , 31 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Nicotiana tabacum Leaf Waste: Morphological Characterization and Chemical-Functional Analysis of Extracts Obtained from Powder Leaves by Using Green Solvents

et al. 2023

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Tobacco cultivation and industrialization are characterized by the production of trillions of pre-harvest and post-harvest waste biomasses each year with the resulting negative effects on the environment. The leaves of blunt, pre-harvest waste, could be further used to obtain bioactive metabolites, i.e., polyphenols and alkaloids, for its potential cosmetic use. This study was conducted to obtain bio-compounds from pre-harvest tobacco leaf waste (var. Virginia) by applying conventional and green solvents (NaDES). Leaves and ground leaf waste were characterized based on their microscopic features. Conventional solvents, such as water, acetone, ethanol, and non-conventional solvents, such as Natural Deep Eutectic Solvents (NaDES), i.e., sucrose:lactic acid (LAS), frutose:glucose:sucrose (FGS), lactic acid:sucrose:water (SALA), choline chloride:urea (CU), and citric acid: propylene glycol (CAP) were used for bioactive extraction from tobacco waste powder. CU, FGS, and acetone/ethanol had similar behavior for the best extraction of alkaloids (6.37–11.23 mg ACE/g tobacco powder). LAS, FGS, SALA, and CU were more effective in phenolic compound extraction than conventional solvents (18.13–21.98 mg AGE/g tobacco powder). Because of this, LAS and SALA could be used to obtain phenolic-enriched extracts with lower alkaloid content rather than CU and FGS. Extracts of the powder obtained with conventional solvent or CU showed a high level of sugars (47 mg/g tobacco powder) The ABTS antioxidant capacity of tobacco leaf powder was higher in the extracts obtained with CU, FGS, and acetone (SC50 1.6–5 µg GAE/mL) while H2O2 scavenging activity was better in the extracts obtained with LAS, CAP and SALA (SC50 3.8–8.7 µg GAE/mL). Due to the biocompatibility of the NaDES with the components of tobacco leaf waste, the opportunity to apply these extracts directly in antioxidant formulations, such as cosmetics, phytotherapic, and other formulations of topic use seems promising. Furthermore, NaDES constituents, i.e., urea and organic acid can also have beneficial effects on the skin.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nicotiana tabacum Leaf Waste: Morphological Characterization and Chemical-Functional Analysis of Extracts Obtained from Powder Leaves by Using Green Solvents

et al. 2023

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“…The liquid biphasic flotation (LBF) system is another emerging and modern liquid–liquid extraction method that faces the difficulties of LLE regarding separation efficiency and low extraction yields. Chia et al [ 36 ] have applied this novel extraction method with low-cost equipment to extract phenolic compounds from kesum plant. The liquid biphasic system integrates the techniques of ATPE [ 37 ] and solvent sublation (a type of adsorption bubble separation technique) [ 38 ].…”

Section: Techniques and Extraction Systems For Phenolic Compoundsmentioning

confidence: 99%

Extraction Systems and Analytical Techniques for Food Phenolic Compounds: A Review

Lama-Muñoz

Contreras

2022

Foods

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Phenolic compounds are highly valuable food components due to their potential utilisation as natural bioactive and antioxidant molecules for the food, cosmetic, chemical, and pharmaceutical industries. For this purpose, the development and optimisation of efficient extraction methods is crucial to obtain phenolic-rich extracts and, for some applications, free of interfering compounds. It should be accompanied with robust analytical tools that enable the standardisation of phenolic-rich extracts for industrial applications. New methodologies based on both novel extraction and/or analysis are also implemented to characterise and elucidate novel chemical structures and to face safety, pharmacology, and toxicity issues related to phenolic compounds at the molecular level. Moreover, in combination with multivariate analysis, the extraction and analysis of phenolic compounds offer tools for plant chemotyping, food traceability and marker selection in omics studies. Therefore, this study reviews extraction techniques applied to recover phenolic compounds from foods and agri-food by-products, including liquid–liquid extraction, solid–liquid extraction assisted by intensification technologies, solid-phase extraction, and combined methods. It also provides an overview of the characterisation techniques, including UV–Vis, infra-red, nuclear magnetic resonance, mass spectrometry and others used in minor applications such as Raman spectroscopy and ion mobility spectrometry, coupled or not to chromatography. Overall, a wide range of methodologies are now available, which can be applied individually and combined to provide complementary results in the roadmap around the study of phenolic compounds.

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“…36,38 Effect of flotation time on separation of ⊍-La Generally, too short a flotation time was not conducive to achieving successful separation, whereas an excessive flotation time would have adverse effects as a result of environmental factors (such as temperature and oxygen). 39 As shown in Fig. 5 Analysis of recovery of UCON and purification of ⊍-La by temperature induction method UCON was the representative example of polymer that was suitable for thermal induced separation, and the recovered polymer had been successfully recycled in the separation of active molecules.…”

Section: Effect Of Flow Velocity On Separation Of ⊍-Lamentioning

confidence: 99%

Direct separation and purification of α‐lactalbumin from cow milk whey by aqueous two‐phase flotation of thermo‐sensitive polymer/phosphate

Jiang

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND: ⊍-lactalbumin (⊍-La) is of great interest to the industry as a result of its excellent functional properties and nutritional value. Aqueous two-phase flotation (ATPF) of thermo-sensitive polymer poly (ethylene glycol-ran-propylene glycol) monobutyl ether (UCON) and KH 2 PO 4 was applied to directly separate and purify ⊍-La from milk whey, which was purposed to simplify the production process and reduced cost of production.RESULTS: The effect of ATPF composition and operating parameters on the flotation efficiency (E) and purity of ⊍-La were investigated. The optimal conditions included 2 min of premixing time, 30 mL min −1 flow velocity and 20 min of flotation time, whereas the composition conditions comprised 35.0 mL 0.18 g mL −1 phosphate solution (containing 10% (cow milk whey/salt solution, v/v) cow milk whey, 50 ppm defoamer and 2 g NaCl) and 5.0 mL of 40% (w/w) UCON solution. Under the optimal conditions, E of ⊍-La was 95.67 ± 1.04% and purity of ⊍-La was 98.78 ± 1.19%. UCON was recovered by a thermally-induced phase separation and reused in next ATPF process without reducing E of ⊍-La. Purified ⊍-La was characterized by several key technologies. The results indicated that ⊍-La in cow milk whey could be directly separated and purified by the ATPF and the purity was satisfactory. Moreover, it was suggested there was no obvious structure difference between the ⊍-La separated by ATPF and the ⊍-La standard.CONCLUSION: The present study enabled the recycling of UCON, providing an effective, economically viable and environmentally friendly approach for the separation and purification of protein.

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Extraction of phenolic compounds from fresh and wilt kesum plant using liquid biphasic flotation

Cited by 16 publications

References 22 publications

Nicotiana tabacum Leaf Waste: Morphological Characterization and Chemical-Functional Analysis of Extracts Obtained from Powder Leaves by Using Green Solvents

Nicotiana tabacum Leaf Waste: Morphological Characterization and Chemical-Functional Analysis of Extracts Obtained from Powder Leaves by Using Green Solvents

Extraction Systems and Analytical Techniques for Food Phenolic Compounds: A Review

Direct separation and purification of α‐lactalbumin from cow milk whey by aqueous two‐phase flotation of thermo‐sensitive polymer/phosphate

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