Extraction of phycobiliprotein pigments from Oscillatoria princeps by using different methods

Abstract

In the current study, one isolation was obtained from the Cyanophyta Oscillatoria princeps which was collected from pond water located in Khor Al-Zubair/Basrah during August 2018. The current study aims at extracting and purifying of some effective pigments from the above isolate, test them chemically. Allophycocyanin (APC) pigments were highest among other pigments in sample removal-oil compared to the quantities of concentrations of phycoerythrin (PE) and phycocyanin (PC) pigments. The stationary phase in the industrial media have produced the largest amount of pigments. Phycobiliproteins were observed through the UV-Visible peaks. Freeze-dried samples or removal-oil showed that phycobiliproteins aren't affected by increasing or decreasing the sonication period. The study showed that it’s possible to use different media for pigments extraction as buffers and acetate buffer has been mastered. The freezing-thawing method is the best compared with other methods, the concentration of phycoerythrin (PE) pigments began to increase gradually from 6-12 days at PC and PE pigments extract, respectively. According to the statistical analysis, there are significance differences in Phycoerythrin. pigments concentration from 9th day to the 12th day.