Extraction of polyphenols in Himanthalia elongata and determination by high performance liquid chromatography with diode array detector prior to its potential use against oxidative stress

Belda, Mariola; Sanchez, Desiré; Bover, Elena; Prieto, B.; Padrón, C; Cejalvo‐Lapeña, Dolores; Lloris, J.M.

doi:10.1016/j.jchromb.2016.09.001

Cited by 31 publications

(20 citation statements)

References 39 publications

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“…and also presented relevant quantities of compounds C, CA, and E ( Table 5). The results here agree with recently published studies focused on the macroalgae Himanthalia elongata (Ochrophyta) which have shown relevant amounts of phenolic compounds increased to levels of mg per gram of freeze-dried algae [42], which seems to be common in many macroalgae species [43]. These contents of phenolic compounds are significantly higher compared to other previously published data [29,30].…”

Section: Phenolic Profile Bysupporting

confidence: 91%

Phenolic Profile and Antioxidant Activity of Crude Extracts from Microalgae and Cyanobacteria Strains

Jerez-Martel

García-Poza

Rodríguez-Martel

et al. 2017

Journal of Food Quality

152

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Aqueous and methanolic extracts of several microalgae (Ankistrodesmus sp., Spirogyra sp., Euglena cantabrica, and Caespitella pascheri) and cyanobacteria (Nostoc sp., Nostoc commune, Nodularia spumigena, Leptolyngbya protospira, Phormidiochaete sp., and Arthrospira platensis) were screened for their radical scavenging activity against the stable radical 1,1-diphenyl-2-picrylhydrazyl. Despite the fact that water was a more efficient solvent to extract greater amount of extractable substances, it seems that methanol was more efficient to extract a selected group of compounds with a higher antioxidant activity. In addition, the identification of 4 simple phenolics (gallic, syringic, protocatechuic, and chlorogenic acids) and the flavonoids (+) catechin and (-) epicatechin was carried out by using reverse phase high performance liquid chromatography. The strain Euglena cantabrica showed the highest concentration of phenolic compounds, particularly gallic and protocatechuic acids (5.87 and 2.97 mg per gram of dried biomass, resp.). Aqueous and methanolic extracts of microalgae Euglena cantabrica also exhibited the highest antioxidant activity, probably due to the presence of the high contents of phenolics.

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Section: Phenolic Profile Bysupporting

confidence: 91%

Phenolic Profile and Antioxidant Activity of Crude Extracts from Microalgae and Cyanobacteria Strains

Jerez-Martel

García-Poza

Rodríguez-Martel

et al. 2017

Journal of Food Quality

152

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“…The increment of acetone above 70% caused a downward tendency in the TPhC of the extracts, suggesting that, in such conditions, the polarity of the solvent is above the ideal for phlorotannin extraction. Other authors demonstrated that concentrations between 40 to 80% of acetone were more effective than pure acetone or water to produce brown algae extracts with high content in phlorotannins [24,29,30,31]. Interestingly, Leyton et al demonstrated that water was the best solvent for phlorotannin extraction in Macrocystis pyrifera [32].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Phlorotannins Extraction from Fucus vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders

et al. 2019

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Phlorotannins are phloroglucinol-based phenolic compounds, occurring particularly in brown macroalgae, that have been recognized for their promising bioactive properties. In this study, the extraction of phlorotannins from Fucus vesiculosus was evaluated with particular emphasis on the influential parameters, including the solvent concentration, solvent-solid ratio, extraction temperature and extraction time, using a single-factor design followed by a Box-Behnken design. The maximum total phlorotannin content, determined using the 2,4-dimethoxybenzaldehyde (DMBA) method, corresponded to 2.92 ± 0.05 mg of phloroglucinol equivalents/g dry seaweed (mg PGE/g DS), and was achieved for extracts carried out with acetone 67% (v/v), a solvent-solid ratio of 70 mL/g and temperature at 25 °C. This crude extract, together with a semi-purified phlorotannin fraction, were further evaluated for their anti-enzymatic capacity against α-glucosidase, α-amylase and pancreatic lipase, both showing promising inhibitory effects, particularly against α-glucosidase for which a greater inhibitory effect was observed compared to the pharmaceutical drug acarbose (IC50 = 4.5 ± 0.8 and 0.82 ± 0.3 μg/mL, respectively, against 206.6 ± 25.1 μg/mL). Additionally, the ultra-high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) analysis carried out on the ethyl acetate fraction revealed the presence of fucols, fucophlorethols, fuhalols and several other phlorotannin derivatives. Moreover, possible new phlorotannin compounds, including fucofurodiphlorethol, fucofurotriphlorethol and fucofuropentaphlorethol, have been tentatively identified in this extract. Overall, this study provides evidence that F. vesiculosus phlorotannin-rich extracts hold potential for the management of the activity of α-glucosidase, α-amylase and pancreatic lipase, which are well known to be linked to metabolic disorders such as diabetes and obesity.

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“…Table 1, Table 2, Table 3 and Table 4 summarize the results of studies on phenolic potential of different brown algae species of different geographical origin: Algeria [39], Australia [28,40,41,42], Brazil [43], China [44,45], Denmark [46], France [47,48,49,50], Iceland [51], India [12,37], Iran [52], Ireland [53,54,55,56,57,58], Japan [59,60,61,62], Korea [63], Lebanon [64], Malaysia [65,66,67], New Zealand [42], Portugal [50], South Korea [68], Spain [69,70,71,72,73], Thailand [74,75], and Tunisia [4,76,77]. The investigated species belong to different orders: Desmarestiales (1 species), Dictyotales (12 species), Ectocarpales (1 species), Fucales (42 species), Laminariales (14 species), Sphacelariales (3 species), and Tilopteridales (1 species).…”

Section: Brown Algae Phenolicsmentioning

confidence: 99%

Phenolic Content of Brown Algae (Pheophyceae) Species: Extraction, Identification, and Quantification

et al. 2019

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Over the last few decades, isolations and chemical characterizations of secondary metabolites with proved biological activities have been of interest for numerous research groups across the world. Phenolics, as one of the largest and most widely distributed group of phytochemicals, have gained special attention due to their pharmacological activity and array of health-promoting benefits. Reports on phenolic potentials of marine algae, especially brown algae (Pheophyceae) that are characterized by the presence of phlorotannins, are still scarce. The aim of this review paper is to provide an overview of current knowledge about phenolic potential of different brown algae species (74 species from 7 different orders). Studies on brown algae phenolics usually involve few species, thus the focus of this review is to provide information about the phenolic potential of reported algae species and to get an insight into some issues related to the applied extraction procedures and determination/quantification methods to facilitate the comparison of results from different studies. The information provided through this review should be useful for the design and interpretation of studies investigating the brown algae as a source of valuable phytochemicals.

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Extraction of polyphenols in Himanthalia elongata and determination by high performance liquid chromatography with diode array detector prior to its potential use against oxidative stress

Cited by 31 publications

References 39 publications

Phenolic Profile and Antioxidant Activity of Crude Extracts from Microalgae and Cyanobacteria Strains

Phenolic Profile and Antioxidant Activity of Crude Extracts from Microalgae and Cyanobacteria Strains

Optimization of Phlorotannins Extraction from Fucus vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders

Phenolic Content of Brown Algae (Pheophyceae) Species: Extraction, Identification, and Quantification

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