Extraction of protein from churpi of yak milk origin: Size reduction, nutraceutical potential and as a wall material for resveratrol

Gani, Asir; Noor, Nairah; Joseph-Leenose-Helen, Jenno; Shah, Asima; Ashraf, Zanoor ul

doi:10.1016/j.fbio.2022.101612

Cited by 11 publications

(4 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is attributed to the higher dissolution/disintegration of the apple protein in the alkaline medium followed by the release of chlorogenic acid [ 15 ]. These results are in accordance with a previous study reported by Gani et al [ 19 ] in which churpi protein was used as wall material for the encapsulation of resveratrol. These results show that the release of chlorogenic acid from the apple protein matrix is pH-dependent and starts its release in the intestines.…”

Section: Resultssupporting

confidence: 93%

“…The encapsulated capsules were characterised by size, polydispersity index, and surface charge using dynamic light scattering. Particle size is an important characteristic for understanding the behaviour of the encapsulated capsules, such as their encapsulation efficiency, release kinetics, solubility, and functionality [ 19 ]. The particle size of the native- (N-Protein) and nano-protein (Us-Protein) was found in the range of 1.2 µm to 484 nm, which significantly ( p < 0.05) increased upon the encapsulation process as presented in Table 1 .…”

Section: Resultsmentioning

confidence: 99%

“…The enhanced EE of the Nano-Chl could be ascribed to the nanosized particles used for encapsulation. Fine particle sizes have good EE because of their ability to produce a film around the bioactive ingredient; hence, there is higher retention of chlorogenic acid [ 18 , 19 ]. Ariyarathna et al [ 24 ] reported the somehow similar results of chickpea protein loaded with folate.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Upscaling of Apple By-Product by Utilising Apple Seed Protein as a Novel Wall Material for Encapsulation of Chlorogenic Acid as Model Bioactive Compound

Gani

Ashraf²,

Shah³

et al. 2022

Foods

View full text Add to dashboard Cite

Encapsulation is a versatile technique used to protect sensitive bioactive compounds under gastrointestinal conditions. In this study, nanoencapsulation of chlorogenic acid into the apple seed protein matrix was performed using the green technique ultrasonication to protect it from harsh gastric conditions and increase its biological activity and bioavailability upon digestion. Both nano (Nano-Chl) and native capsules (NT-Chl) were characterised by particle size, charge, structure, and morphology. The encapsulation efficiency, release behaviour, antioxidant and antidiabetic properties were also evaluated. The experimental results show that the particle size of the NT-Chl and Nano-Chl was found in the range of 1.4 µm to 708 nm. The encapsulation efficiency was found to be 69% and 80% for NT-Chl and Nano-Chl, respectively. Furthermore, an in vitro digestion study revealed that Nano-Chl showed controlled-release behaviour under simulated intestinal conditions in comparison to NT-Chl. Moreover, Nano-Chl showed enhanced antioxidant and antidiabetic activity in comparison to NT-Chl after simulated digestion. It was concluded that the protein from apple seeds could be utilised as a functional ingredient itself or as a wall material for the encapsulation of sensitive bioactive compounds. Furthermore, these encapsulated particles can be fortified into different food formulations for the development of functional food.

show abstract

Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Upscaling of Apple By-Product by Utilising Apple Seed Protein as a Novel Wall Material for Encapsulation of Chlorogenic Acid as Model Bioactive Compound

Gani

Ashraf²,

Shah³

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…First, the chemical composition of ZIF‐8 NPs, Res@ZIF‐8 NPs and Res@ZIF‐8/TA NPs was detected by FTIR spectroscopy, as shown in Figure 2A. Compared with ZIF‐8, Res@ZIF‐8 NPs showed a new absorption peak at 965.3 cm −1 (double bond), 22 indicating that Res was successfully encapsulated in the framework of ZIF‐8 NPs. Compared with Res@ZIF‐8 NPs, the wide peak at 3315.9 cm −1 of Res@ZIF‐8/TA NPs was from the absorption peak of the OH stretching vibration peak in TA, 23 confirming that TA successfully was attached to Res@ZIF‐8/TA NPs.…”

Section: Resultsmentioning

confidence: 99%

pH‐responsive resveratrol‐loaded ZIF‐8 nanoparticles modified with tannic acid for promoting colon cancer cell apoptosis

Sun,

Li,

Yuan

et al. 2023

J Biomed Mater Res

View full text Add to dashboard Cite

Resveratrol (Res) is known for its potential in treating various types of cancers, with a particular advantage of causing minimal toxic side effects. However, its clinical application is constrained by challenges such as poor bioavailability, low water solubility, and chemical instability in neutral and alkaline environments. In light of these limitations, we have developed a pH‐responsive drug delivery nanoplatform, Res@ZIF‐8/TA NPs, which exhibits good biocompatibility and shows promise for in vitro cancer therapy. Benefiting from the mild reaction conditions provided by zeolitic imidazolate frameworks (ZIFs), a “one‐pot method” was used for drug synthesis and loading, resulting in a satisfactory loading capacity. Notably, Res@ZIF‐8/TA NPs respond to acidic environments, leading to an improved drug release profile with a controlled release effect. Our cell‐based experiments indicated that tannic acid (TA) modification enhances the biocompatibility of ZIFs. 3‐(4,5)‐dimethylthiahiazo (‐z‐y1)‐3,5‐di‐ phenytetrazoliumromide (MTT assay), Hoechst 33342/PI staining, cell scratch assay, Transwell and Reverse Transcription quantitative PCR (RT‐qPCR) assays further demonstrated that Res@ZIF‐8/TA NPs inhibited colon cancer cell migration and invasion, and promoted apoptosis of colon cancer cells, suggesting a therapeutic potential and demonstrating anti‐cancer properties. In conclusion, the Res@ZIF‐8/TA NPs pH‐responsive drug delivery systems we developed may offer a promising avenue for cancer therapy. By addressing some of the challenges associated with Res‐based treatments, this system could contribute to advancements in cancer therapeutics.

show abstract

Exploring the multimodal health‐promoting properties of resveratrol: A comprehensive review

Faisal,

Mazhar,

Batool

et al. 2024

Food Science & Nutrition

View full text Add to dashboard Cite

Resveratrol, a natural polyphenol in various plants, has gained significant attention for its potential health‐promoting properties. It has been demonstrated, after reviewing various clinical and in vitro studies, that resveratrol possesses potent antioxidant potential. Resveratrol demonstrates cellular component protection by directly neutralizing free radicals (FRs) and enhancing the expression of natural antioxidant enzymes, thereby mitigating oxidative damage to proteins, lipids, and nucleic acids. Clinical trials have shown promising results, indicating that resveratrol supplementation can enhance antioxidant defenses and reduce oxidative damage markers in various populations. In addition to its antioxidant effects, resveratrol exhibits potent anti‐inflammatory properties. It can modulate key inflammatory pathways, such as nuclear factor‐kappa B (NF‐κB) and mitogen‐activated protein kinases (MAPKs), thereby suppressing the production of pro‐inflammatory cytokines and chemokines. Furthermore, resveratrol's multimodal effects extend beyond its antioxidant and anti‐inflammatory properties. It has been discovered to exert regulatory effects on various cellular processes, including apoptosis, cell cycle progression, angiogenesis, and immunological responses. The primary aim of this review paper is to provide a thorough overview of the current knowledge on resveratrol, including its chemical composition, bioaccessibility, clinical effectiveness, and utilization in nanotechnology to enhance its bioavailability. From future perspectives, revising the administration methods for certain contexts and understanding the underlying systems responsible for resveratrol's effects will require further inquiry. For the highest potential health results, advanced trial‐based research is necessary for combinational nano‐delivery of resveratrol.

show abstract

Extraction of protein from churpi of yak milk origin: Size reduction, nutraceutical potential and as a wall material for resveratrol

Cited by 11 publications

References 69 publications

Upscaling of Apple By-Product by Utilising Apple Seed Protein as a Novel Wall Material for Encapsulation of Chlorogenic Acid as Model Bioactive Compound

Upscaling of Apple By-Product by Utilising Apple Seed Protein as a Novel Wall Material for Encapsulation of Chlorogenic Acid as Model Bioactive Compound

pH‐responsive resveratrol‐loaded ZIF‐8 nanoparticles modified with tannic acid for promoting colon cancer cell apoptosis

Exploring the multimodal health‐promoting properties of resveratrol: A comprehensive review

Contact Info

Product

Resources

About