2021
DOI: 10.1002/jsfa.11575
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Extraction of selected prohealth substances from Curcuma longa and Zingiber officinale in artificial digestive juices

Abstract: BACKGROUND The study determined the content of selected bioelements, toxic metals and phenolic acids occurring in Curcuma longa L. and Zingiber officinale Rosc. Natural raw materials in the form of powder, slices, dried and fresh rootstock, tablets and capsules were analyzed. To determine the actual bioavailability of the metals and phenolic acids present in the raw materials, they were extracted into artificial digestive juices (artificial gastric and intestinal juices). RESULTS The maximum amounts of bioelem… Show more

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“…The extracts were dissolved quantitatively in HPLC-grade methanol and re-filtered through membrane filters. The prepared methanol extracts were used for determining the content of phenolic acids using RP-HPLC according to an optimized procedure described elsewhere [ 21 ]. The process was performed using an HPLC (Merck-Hitachi, Merck KGaA, Darmstadt, Germany) apparatus equipped with the following: an L-2200 autosampler, an L-2130 pump, a LiChrospher RP-18e column (250 mm × 4 mm, 5 µm) thermostated at 25 °C, an L-2350 column oven, and an L-2455 diode array detector at a UV range of 200–400 nm.…”
Section: Methodsmentioning
confidence: 99%
“…The extracts were dissolved quantitatively in HPLC-grade methanol and re-filtered through membrane filters. The prepared methanol extracts were used for determining the content of phenolic acids using RP-HPLC according to an optimized procedure described elsewhere [ 21 ]. The process was performed using an HPLC (Merck-Hitachi, Merck KGaA, Darmstadt, Germany) apparatus equipped with the following: an L-2200 autosampler, an L-2130 pump, a LiChrospher RP-18e column (250 mm × 4 mm, 5 µm) thermostated at 25 °C, an L-2350 column oven, and an L-2455 diode array detector at a UV range of 200–400 nm.…”
Section: Methodsmentioning
confidence: 99%