Extraction of small molecule from human plasma by prototyping 3D printed sorbent through extruded filament for LC-MS/MS analysis

Adye, Daya Raju; Ponneganti, Srikanth; Malakar, Tushar Kanti; Radhakrishnanand, P.; Murty, Upadhyayula Suryanarayana; Banerjee, Subham; Borkar, Roshan M.

doi:10.1016/j.aca.2021.339142

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…Various 3D-printed devices have been employed to aid in extracting analytes from biological matrices. These devices serve as sorbents, sorbent holders, membrane separators, phase separators, and extraction chambers [ 2 , 33 , 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…The activation process using water resulted in the formation of a microporous structure on the surface of the sorbent by removing PVA [ 2 , 35 ]. Qualitative and quantitative DSC analyses were employed to measure the partial removal of PVA crystals from the extraction device.…”

Section: Resultsmentioning

confidence: 99%

“…However, this endothermic peak was not observed in the thermogram of the washed sorbent, indicating the removal of PVA from the extraction device during the activation process. This indicated that PVA was either completely or partially removed from the polymer composite [ 2 ], which involved interactions between the drug molecules and polymers and reversible adsorption on the surface of the extraction device. Prototyped 3D extraction devices formed pores after activation for 10 min with double-distilled water and sonication, as observed in Figure 3 after the sorbent activation.…”

Section: Resultsmentioning

confidence: 99%

“…FFF 3D printing is preferred due to its affordability and ability to print various thermoplastic polymeric filaments into tangible prototypes, including the ability to print personalized dosage forms and customized shapes. Recently, the extraction of small molecules from plasma samples using 3D printing technology has emerged, most notably in analytical chemistry [ 2 ]. Several analytical prototypes have been developed using 3D printing technology, including porous media columns [ 3 ], 3D-printed extraction sorbents for sample extraction from biological matrices [ 4 ], cooling interfaces for ultraviolet-light-emitting diode-based optical detectors [ 5 ], sensors [ 6 ], and microfluidic devices [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of NSAIDs in Rat Plasma Using 3D-Printed Sorbents by LC-MS/MS: An Approach to Pre-Clinical Pharmacokinetic Studies

et al. 2023

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Analytical sample preparation techniques are essential for assessing chemicals in various biological matrices. The development of extraction techniques is a modern trend in the bioanalytical sciences. We fabricated customized filaments using hot-melt extrusion techniques followed by fused filament fabrication-mediated 3D printing technology to rapidly prototype sorbents that extract non-steroidal anti-inflammatory drugs from rat plasma for determining pharmacokinetic profiles. The filament was prototyped as a 3D-printed sorbent for extracting small molecules using AffinisolTM, polyvinyl alcohol, and triethyl citrate. The optimized extraction procedure and parameters influencing the sorbent extraction were systematically investigated by the validated LC-MS/MS method. Furthermore, a bioanalytical method was successfully implemented after oral administration to determine the pharmacokinetic profiles of indomethacin and acetaminophen in rat plasma. The Cmax was found to be 0.33 ± 0.04 µg/mL and 27.27 ± 9.9 µg/mL for indomethacin and acetaminophen, respectively, at the maximum time (Tmax) (h) of 0.5–1 h. The mean area under the curve (AUC0–t) for indomethacin was 0.93 ± 0.17 µg h/mL, and for acetaminophen was 32.33± 10.8 µg h/mL. Owing to their newly customizable size and shape, 3D-printed sorbents have opened new opportunities for extracting small molecules from biological matrices in preclinical studies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of NSAIDs in Rat Plasma Using 3D-Printed Sorbents by LC-MS/MS: An Approach to Pre-Clinical Pharmacokinetic Studies

et al. 2023

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“…The 3D printing drug has been developed using melt extrusion deposition (MED) to treat rheumatoid arthritis. In parallel, recent publications in the field of pharmaceutical sciences using other methods of 3D printing such as fused deposition modeling (FDM) have increased 19–23 . For example, FDM technique has been applied to fabricate 3D printed versions of Acetaminophen, Prednisolone, Aspirin, Captopril, Nifedipine, and other pills 13,24–28 …”

Section: Introductionmentioning

confidence: 99%

Facile fabrication of an extended‐release tablet of Ticagrelor using three dimensional printing technology

Rastpeiman,

Panahi,

Akrami

et al. 2023

J Biomedical Materials Res

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The objective of the study was to fabricate tailored extended‐release tablets of blood thinner Ticagrelor as once‐daily dosing using additive manufacturing for better compliance in heart failure therapy. The solid work design of the tablet was printed using hot melt extrusion (HME) based 3D printing by optimized mixture of Eudragit RS‐100, plasticizer and drug for producing extrudable and printable filaments. FTIR and TGA results showed no covalent interaction among ingredients and no decomposition during HME process, respectively. Friability, weight variation, assay and content uniformity tests met USP requirements, while the mean hardness of the tablets was calculated in a value between 40 and 50 kg. According to DSC and XRD results, the crystallinity state of the Ticagrelor was converted to an amorphous one in the tablet matrix. Smooth surfaces with multiple deposited layers were observed using SEM. In comparison, the maximum Ticagrelor release of 100% after 120 min from Brilinta® tablets was decreased to 97% in 400 min from the 3D tablet at infill of 90%. Korsmeyer‐Peppas kinetic model showed the drug release mechanism is affected by diffusion and swelling. In general, fabrication of the extended‐release 3D printed tablet of Ticagrelor using HME‐based‐additive manufacturing has the potential to provide specific doses with tailored kinetic release for personalized medicine, improving adherence at point‐of‐care.

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Polymeric amorphous dispersion as a carrier to enhance the in vitro dissolution in vivo pharmacokinetics and cytotoxicity of niclosamide

Kolimi,

Padakanti,

Kolagani

et al. 2023

Polymers for Advanced Techs

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Amorphous solid dispersion is one of the recent technologies that attracted the pharmaceutical industry to deliver poorly soluble drugs. However, the selection of polymeric carriers and characterization of drug‐polymer interactions need to be performed systemically to develop a stable amorphous delivery system. Solubility parameter analysis was used to screen the polymers. Hot melt extrusion (HME) was used to prepare the polymeric amorphous dispersion. Drug polymer interactions were identified by attenuated total reflection spectroscopy. Solid‐state transformations were characterized by differential scanning calorimetry and powder X‐ray diffraction. In vitro drug release was performed under non‐sink conditions. In vivo pharmacokinetic studies were performed on rats, and MCF‐7 and A‐549 cell lines were used to study the cytotoxicity. Polymer miscibility studies indicated polyvinylpyrrolidone (PVPK17) as a suitable polymer, and 180°C at 50 rpm was the ideal condition in the HME. The analytical tools confirmed the conversion of niclosamide (NCL) to an amorphous state and the existence of hydrogen bond interactions between the drug and the polymer. Prepared solid dispersions were stable for 3 months and showed about 214‐fold improvement in solubility and around 10‐fold improvement in dissolution over pure crystalline NCL. Pharmacokinetic studies showed a 4.04‐fold enhancement in Cmax and a 2.05‐fold enhancement in the area under the plasma concentration‐time curve (AUC0‐24h) compared to crystalline NCL. Cytotoxicity studies also showed a better toxicity profile with a reduction in the IC50 values of NCL in the amorphous solid dispersions. These encouraging findings indicate that polymeric amorphous solid dispersions could be a commercially feasible option to deliver poorly soluble drugs like NCL using HME.

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Extraction of small molecule from human plasma by prototyping 3D printed sorbent through extruded filament for LC-MS/MS analysis

Cited by 14 publications

References 23 publications

Analysis of NSAIDs in Rat Plasma Using 3D-Printed Sorbents by LC-MS/MS: An Approach to Pre-Clinical Pharmacokinetic Studies

Analysis of NSAIDs in Rat Plasma Using 3D-Printed Sorbents by LC-MS/MS: An Approach to Pre-Clinical Pharmacokinetic Studies

Facile fabrication of an extended‐release tablet of Ticagrelor using three dimensional printing technology

Polymeric amorphous dispersion as a carrier to enhance the in vitro dissolution in vivo pharmacokinetics and cytotoxicity of niclosamide

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