Extraction of Two Albumin Fractions from Amaranth Grains: Comparison of Some Physicochemical Properties and the Putative Localization in the Grains

Konishi, Yôtarô; Horikawa, Kazuko; Oku, Yoko; Azumaya, Junko; Nakatani, Nobuji

doi:10.1080/00021369.1991.10857150

Cited by 12 publications

(12 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, globulin appears to be somehow protected by the alcalase treatment. A similar behaviour was reported by Konishi et al (1991): they observed that albumin 1 was decomposed by pronase, but not albumin 2 (actually globulin-p) because the latter is associated with protein-bodies. Profiles by SDS-PAGE have shown that the isolated protein-bodies from amaranth grain contain globulin and albumin 2.…”

Section: Dh and Ace-inhibitory Activity Of Albumin 1 And Globulinsupporting

confidence: 75%

“…In addition, glutelins, storage-aggregated proteins, are only soluble in alkaline or acidic media. Konishi et al (1991) described two types of albumin in amaranth: albumin 1, removed with water and/or saline solutions; and albumin 2, extracted with water after the flour had been treated with saline solutions in order to remove albumin 1 and globulins. Albumin 2, recently called globulin-p, is scarce.…”

Section: Introductionmentioning

confidence: 99%

“…They comprise globulin (11S-globulin and globulin-p) and glutelins, which present different solubility in aqueous solvents. The 11S-globulin is extracted by treating the flour with a saline, neutral buffer (C = 0.5 M) and globulin-p (formerly named albumin 2 by Konishi, Horikawa, Oku, Azumaya, & Nakatani, 1991) is extracted with water after the saline treatment of the flour. In addition, glutelins, storage-aggregated proteins, are only soluble in alkaline or acidic media.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Angiotensin I-converting enzyme-inhibitory peptide fractions from albumin 1 and globulin as obtained of amaranth grain

et al. 2009

View full text Add to dashboard Cite

Section: Dh and Ace-inhibitory Activity Of Albumin 1 And Globulinsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Angiotensin I-converting enzyme-inhibitory peptide fractions from albumin 1 and globulin as obtained of amaranth grain

et al. 2009

View full text Add to dashboard Cite

“…Additionally, it has also been reported that globulin-P is associated with protein-bodies that do not allow easy access to hydrolytic enzymes. Konishi et al (1991) observed that Glo is nowhere as prone to proteolysis as Alb because the former can be found inside protein bodies and this slows down proteolysis during the first 12 h. Condés et al (2009) found that a polypeptide of 45 kDa from 7S globulin was more resistant to the trypsin action (serine protease) and Vecchi and Añón (2009) reported a great resistance to alcalase hydrolysis of hydrophobic B polypeptides of 11S globulin.…”

Section: Resultsmentioning

confidence: 99%

Dipeptidyl peptidase IV inhibitory activity of protein hydrolyzates from <i>Amaranthus hypochondriacus</i> L. Grain and their influence on postprandial glycemia in Streptozotocin-induced diabetic mice.

Jorge¹,

Raúl²,

Isabel³

et al. 2015

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Background: Type 2 diabetes is a chronic metabolic disorder. Recently, dipeptidyl peptidase IV (DPP-IV) inhibitors that protect incretin hormones from being cleaved by DPP-IV have been used as drugs to control glycemia. This study examined the potential hypoglycemic effect of amaranth grain storage protein hydrolyzates to control postprandial glycemia in streptozotocin (STZ)-induced diabetic mice as a model system of diabetes, and their inhibition mode on the enzyme. Material and Methods: Amaranth grain proteins were isolated and hydrolyzed and fractionated by gel filtration. The DPP-IV inhibitory activity of hydrolyzates as well as their kinetic parameters were assessed. Selected hydrolyzates (300 mg/kg body weight) were administered in a single administration-study (SAS) or in the same concentration during a four-week chronic daily dosing study (FWCDDS) in order to observe the effect on postprandial glycemia of diabetic mice. Results: Albumin 1, Globulin and Glutelin hydrolyzates (GluH) competitively inhibited DPP-IV in vitro (Ki= 0.11-5.61 mg/mL). GluH called Glu.III (IC 50 = 0.12±0.01 mg/mL) considerably inhibited DPP-IV activity. GluH identified as GluH24 improved glucose tolerance significantly (p<0.05), with remarkable increments in plasma insulin in SAS and FWCDDS (1.25 and 2.25 mg/mL, respectively). This effect could be compared to the one obtained from the mice group that was administered Sitagliptin (580 mg/kg body weight) as positive control (p<0.05). Conclusion: Amaranth Glutelin hydrolyzates yielded the highest enzyme inhibitory activity reported not only in vitro, but also in the STZ-induced diabetic mice in order to control postprandial glycemia.

show abstract

“…We have recently observed two albumin fractions (Alb-1 and Alb-2) in amaranth grains (11). Alb-2 was extracted with water after extracting Alb-1 and water sometimes shows an acidic pH by dissolving C02, depending on temperature or duration of storage of distilled water used.…”

mentioning

confidence: 99%

A Pitfall in Determining the Globulin/Albumin Ratio in Amaranth Grains.

Konishi

Azumaya

Horikawa

et al. 1992

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

Self Cite

View full text Add to dashboard Cite

SummaryIn amaranth grains (Amaranthus hypochondriacus L. and Amaranthus cruentus L.), there were two albumin (Alb) fractions, Alb-1 and Alb-2; Alb-1 was extracted with water or 0.5M NaCl, and Alb-2 was extracted with water after extracting Alb-1 and globulin. The amount of Alb-2 comprised about 30% of the total albumin and globulin content. Little or no measurable protein corresponding to the amaranth Alb-2 was extracted from seeds of buckwheat (Fagopyrum esculentum Moench), quinoa (Chenopodium quinoa L.), or barley (Hordeum vulgare L. cv Sekitorisai-1). That the globulin/albumin ratio (G/A) in amaranth grains has been conflicting among investigatiors probably accounts for the consideration of Alb-2 during extraction. We here report the revised G/ A ratio of 0.3-0.4.

show abstract

Extraction of Two Albumin Fractions from Amaranth Grains: Comparison of Some Physicochemical Properties and the Putative Localization in the Grains

Cited by 12 publications

References 8 publications

Angiotensin I-converting enzyme-inhibitory peptide fractions from albumin 1 and globulin as obtained of amaranth grain

Angiotensin I-converting enzyme-inhibitory peptide fractions from albumin 1 and globulin as obtained of amaranth grain

Dipeptidyl peptidase IV inhibitory activity of protein hydrolyzates from <i>Amaranthus hypochondriacus</i> L. Grain and their influence on postprandial glycemia in Streptozotocin-induced diabetic mice.

A Pitfall in Determining the Globulin/Albumin Ratio in Amaranth Grains.

Contact Info

Product

Resources

About