Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers

Blosser, Renée S; Gray, Kendall M.

doi:10.1016/s0167-7012(99)00136-0

Cited by 199 publications

(140 citation statements)

References 24 publications

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“…Similarly to other clinically significant multidrug-resistant microorganisms, C. violaceum shows resistance against numerous antibiotics and drug efflux pump genes (18) and thus represents a model for antibacterial screening. C. violaceum also uses a LuxR/I-type QS system (19), where CviI is an N-hexanoyl-L-homoserine lactone (C 6 -AHL) synthase, and CviR is a cytoplasmic receptor protein (DNA) binding transcription factor) that activates gene expression following binding to a diffusible autoinducer C 6 -AHL (20). As the purple pigment violacein is under the control of this system, the inhibition of pigment production indicates the presence of anti-QS activity; therefore, C. violaceum bioassays are suitable for investigations of this type (13)(14)(15)(16)(17).…”

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confidence: 99%

Antibacterial and quorum sensing regulatory activities of some traditional Eastern-European medicinal plants

2014

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The increasing resistance of human and animal bacterial pathogens to a wide spectrum of antibiotics has become an emerging problem in the 21st century (1). In view of the slow progress in the development of antibiotic classes that can overcome drug-resistance mechanisms, the next generation of safe and effective antimicrobial drugs is likely to be The objective of this study was to screen extracts of twenty Eastern European medicinal plants, using wild-type and reporter Chromobacterium violaceum bioassays, for novel components that target bacterial cells and their quorum sensing (QS) communication systems. Three types of activity and their combinations were revealed: (i) direct antimicrobial growth-inhibitory activity, (ii) non-specific and specific pro-QS activities, (iii) anti-QS activity. Among seven plant extracts showing direct growth-inhibitory activity, the strongest effect was shown by Arctostaphylos uva--ursi (bearberry) leaves. Many plants stimulated violacein production by wild-type C. violaceum ATCC 31532 in a non-specific manner, and only the herb Bidens tripartita (three-lobe beggarticks) contained compounds that mimic acyl-homoserine lactone and operated as a QS agonist. Anti-QS activity was found in eleven plants including Quercus robur (oak) cortex, Betula verrucosa (birch) buds and Eucalyptus viminalis (Manna Gum) leaves. Subsequent statistical analysis showed differences between antimicrobial and anti-QS activities, whereas both activities were defined by phylogenetic position of medical resource plant. Finally, extract from Quercus robur cortex revealed at least two fractions, showing different anti-QS mechanisms. These data confirm that multicomponent anti-infectious mechanisms are used by plants, which may be useful for drug development.

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confidence: 99%

Antibacterial and quorum sensing regulatory activities of some traditional Eastern-European medicinal plants

2014

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“…The solution was vortexed vigorously for 30 s to completely solubilize the violacein, then centrifuged at 12000 rpm for 10 min to remove the cells (Choo et al ., 2006). Next, 200 μl of the violacein‐containing supernatant was added to 96‐well flat‐bottomed microplates, with three wells used per solution; absorbance was read with a spectrophotometer (Infinite ® 200 PRO, Tecan, Groedig, Salzburg, Austria) at a wavelength of 585 nm (Blosser and Gray, 2000). …”

Section: Methodsmentioning

confidence: 99%

Antibiofilm activity substances derived from coral symbiotic bacterial extract inhibit biofouling by the model strainPseudomonas aeruginosaPAO1

Song

Cai

Lao

et al. 2018

Microbial Biotechnology

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SummaryThe mitigation of biofouling has received significant research attention, with particular focus on non‐toxic and sustainable strategies. Here, we investigated quorum sensing inhibitor (QSI) bacteria as a means of controlling biofouling in a laboratory‐scale system. Approximately, 200 strains were isolated from coral (Pocillopora damicornis) and screened for their ability to inhibit quorum sensing (QS). Approximately, 15% of the isolates exhibited QSI activity, and a typical coral symbiotic bacterium, H12‐Vibrio alginolyticus, was selected in order for us to investigate quorum sensing inhibitory activity further. Confocal microscopy revealed that V. alginolyticus extract inhibited biofilm formation from Pseudomonas aeruginosa PAO1. In addition, the secondary metabolites of V. alginolyticus inhibited PAO1 virulence phenotypes by downregulating motility ability, elastase activity and rhamnolipid production. NMR and MS spectrometry suggested that the potential bioactive compound involved was rhodamine isothiocyanate. Quantitative real‐time PCR indicated that the bacterial extract induced a significant downregulation of QS regulatory genes (lasB, lasI, lasR, rhlI, rhlR) and virulence‐related genes (pqsA, pqsR). The possible mechanism underlying the action of rhodamine isothiocyanate analogue involves the disruption of the las and/or rhl system of PAO1. Our results highlight coral microbes as a bioresource pool for developing QS inhibitors and identifying novel antifouling agents.

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“…Violacein quantification: Quantitative evaluation of QS inhibitory activity was carried out following the procedure described by Blosser et al 12 , in which the pigment violacein is extracted in ethanol from liquid culture of C. violaceum ATCC 12472 and measured by molecular absorption at 585 nm. The 100% value was assigned to the violacein concentration produced by control cultures grown without inhibitor (n=3).…”

Section: Qualitative Qs Inhibitionmentioning

confidence: 99%

Inhibition of Quorum Sensing by Drimane Lactones From Chilean Flora

Carcamo¹,

Silva²,

Becerra³

et al. 2014

J. Chil. Chem. Soc.

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Fifteen native Chilean plants were evaluated against the quorum sensing (QS) biosensor AI-1, Chromobacterium violaceum ATCC 12472. Three extracts showed inhibitory activity of QS: Drymis winteri J.R, Psoralea glandulosa L and Peumus boldus. Purification of secondary metabolites from barks of Drimys winteri showed that drimane sesquiterpenes were the main compounds in the tree. Polygodial, drimenol, isodrimeninol, drimenin, isodrimenin, cinnamolide and valdiviolide were isolated and evaluated in QS inhibition assays. Two α,β unsaturated lactones, cinnamolide and valdiviolide, with the carbonyl on position 12 of the drimane skeleton were found to be inhibitors of QS; other drimane lactones were not active.

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Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers

Cited by 199 publications

References 24 publications

Antibacterial and quorum sensing regulatory activities of some traditional Eastern-European medicinal plants

Antibacterial and quorum sensing regulatory activities of some traditional Eastern-European medicinal plants

Antibiofilm activity substances derived from coral symbiotic bacterial extract inhibit biofouling by the model strainPseudomonas aeruginosaPAO1

Inhibition of Quorum Sensing by Drimane Lactones From Chilean Flora

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