Extraction, purification, and characterisation of the flavonoids from Opuntia milpa alta skin

Abstract

Cai W., Gu X., Tang J. (2010): Extraction, purification, and characterisation of the flavonoids from Opuntia milpa alta skin. Czech J. Food Sci., 28: 108-116.The flavonoids contained in Opuntia milpa alta skin were extracted, purified, and identified. Based on single factor experimental results, the extraction parameters were optimised by using orthogonal design and variance analysis. The results show that the maximum yield of the flavonoids (5.55 mg/g dry weight) can be obtained with 80% ethanol (v/v), extraction temperature of 90°C, and the solvent to raw material ratio of 25:1. After purification with AB-8 resin, the main components of the extract were characterised as isorhamnetin 3-O-(2, 6-dirhamnosyl)glucoside and isorhamnetin 3-O-d-rutinoside by employing liquid chromatography-mass spectrometry and ultraviolet-visible spectrometry.

In vitro evaluation of the fermentation of added‐value agroindustrial by‐products: cactus pear (Opuntia ficus‐indica L.) peel and pineapple (Ananas comosus) peel as functional ingredients

“…The percentage of ABTS scavenging activity or antioxidant activity for cactus pear peel flours was higher than for pineapple peel flour (89% and 69%, respectively), with the resultant TEAC value being higher for cactus pear peel flour (2.6 and 1.5, respectively). The use of cactus pear peel as a source of functional ingredient such as fibre (Bensadón et al ., ), antioxidants (Fernández‐López et al ., ; Cardador‐Martínez et al ., ) and flavonoids (Cai et al ., ) has been recently reported. Similarly, pineapple peel has also been studied as functional ingredient as a source of fibre (Huang et al ., ), polyphenols (Larrauri et al ., ) or compounds against oxidative stress (Erukainure et al ., ).…”

Beneficial effect ofCitrus limonpeel aqueous methanol extract on experimentally induced urolithic rats

“…The extraction started with removal of essential oils by n-hexane and chloroform was used to remove other non-polar compounds. The remaining polar compounds and flavonoids were extracted with ethanol at a temperature of about 90 C and partitioned with aqueous methanol and n-hexane as performed by Cai et al (2010). In order to observe the mechanism behind the antilithogenic role of lemon peel, we studied the composition of the LP extracts using GC-MS analysis before exhibiting its antilithogenic potential.…”

The optimization of phenolic compounds extraction from cactus pear (Opuntia ficus-indica) skin in a reflux system using response surface methodology

“…Opuntia ficus-indica (O. ficus-indica, cactus pear) is a cactus well adapted to arid and semiarid conditions [9] . The central part of Mexico hosts the greatest diversity of this cactus in the world.…”

“…The average radical inhibition between treatments 5 to 12 was 92%. The higher values were detected using extracts obtained under conditions of treatments [5][6][7][8][9][10][11][12] (Figure 2). …”