Extraction, purification and preliminary characterization of polysaccharides from Kadsura marmorata fruits

Wang, Hongjun; Jiang, Hong; Wang, Shude; Li, Xinguo; Yao, Dan; Dong, Jinqiao; Zhou, Tiezhong; Liu, Yingzi; Gao, Shenyang; Li, Li; Deng, Xuming

doi:10.1016/j.carbpol.2012.11.060

Cited by 11 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further UAE parameters coupling with RSM has become a new avenue for the optimization of yield of polysaccharides. The ratio of polysaccharides extracted from Kadsura marmorata fruit powder was 2.7735 ± 0.068%, where the optimal conditions were extraction time for 3 h, liquid–solid ratio 27:1 and temperature of 98 °C (Wang et al., 2013). Cui et al.…”

Section: Resultsmentioning

confidence: 99%

Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Zhang

et al. 2021

Journal of Food Science

View full text Add to dashboard Cite

In this study, response surface methodology was employed to optimize the ultrasound‐assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP). The optimal conditions for UAE of PSMP were: liquid–solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%. The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography‐mass spectrometry, Fourier‐transfer infrared spectroscopy, and nuclear magnetic resonance detections. Scanning electron microscopy analysis showed that many holes were formed on the surface of PSM after UAE. The antioxidant activities of PSMP were investigated using various assays in vitro. The results suggested that PSMP is potential natural resource of antioxidants for medicine and functional foods. Practical Application The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources. With consumer demands for newly developed foods with natural, wholesome ingredients are increasing nowadays. This study provides effective reference for in‐depth research on other medicine‐food dual‐use resources. Ultrasound‐assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving. In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Zhang

et al. 2021

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…The molar percentage of GalA exceeded 70%, which is consistent with the uronic acid content test results (79.80 ± 1.98%). The polysaccharides of Schisandra fruits of the same genus were previously reported to be mainly composed of GalA and xylose ( 11 ). These results suggested that KCFP is an acidic heteropolysaccharide composed of homogalacturonic acid.…”

Section: Resultsmentioning

confidence: 98%

Physicochemical Characterization and Antioxidant and Hypolipidaemic Activities of a Polysaccharide From the Fruit of Kadsura coccinea (Lem.) A. C. Smith

et al. 2022

View full text Add to dashboard Cite

Kadsura coccinea fruit, a novel fruit resource, has attracted wide interest, but the physicochemical characteristics and biological activities of its polysaccharides remain unclear. This study investigated the physicochemical properties of a polysaccharide extracted from K. coccinea fruit polysaccharide (KCFP) and evaluated its antioxidant and hypolipidaemic activities in vitro and in vivo. KCFP is an amorphous, thermally stable pectin heteropolysaccharide with an average molecular weight of 204.6 kDa that is mainly composed of mannose, rhamnose, glucose, galactose, xylose, arabinose, galacturonic acid (molar percentage >70%) and glucuronic acid. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging assays and an iron reducing antioxidant power assay showed that KCFP has strong antioxidant capacity, while the bile acid binding assay showed that KCFP has hypolipidaemic potential in vitro. The antioxidant and hypolipidaemic activities of KCFP were further evaluated in high-fat diet-induced hyperlipidaemic mice. KCFP significantly increased the activities of superoxide dismutase, glutathione peroxidase and catalase, decreased the malondialdehyde content, significantly reduced the total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) levels, and increased the amount of high-density lipoprotein cholesterol (HDL-C). These findings suggest that KCFP could be used as a functional food to remedy oxidative damage and hyperlipidaemia.

show abstract

“…Sometimes complete reaction mixture can be injected directly into the gas chromatograph, thus avoiding any cleanup stages. There are many silylation reagents that have been applied for the analysis of carbohydrates in sweet medicines at different temperatures for different reaction times (19)(20)(21)(22). Hexamethyldisilazane (HMDS), N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), trimethylchlorosilane (TMCS) and N-methyl-Ntrimethylsilyltrifluoroacetamide (MSTFA) are the most commonly used reagents.…”

Section: Qualitative Analysis Of Monosaccharidesmentioning

confidence: 99%

“…GC analysis despite time consuming for derivatization procedure remains the finest method for precise estimation of uronic acids (41,42). The derivatives including acetates (43), TMS derivatives (22,44), oximes derivatives (45), and alditol acetates (26) have been used for the analysis of sugar acids. However, because hydroxyl and carboxyl groups simultaneously exist in one molecule, different lactones will be formed, and furthermore, coupled with anomeric centre, complex chromatograms will be generated when uronic acids presented in the samples.…”

Section: Sugar Acidsmentioning

confidence: 99%

Quality control of sweet medicines based on gas chromatography-mass spectrometry

Zhao

et al. 2015

DD&T

View full text Add to dashboard Cite

Extraction, purification and preliminary characterization of polysaccharides from Kadsura marmorata fruits

Cited by 11 publications

References 13 publications

Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Physicochemical Characterization and Antioxidant and Hypolipidaemic Activities of a Polysaccharide From the Fruit of Kadsura coccinea (Lem.) A. C. Smith

Quality control of sweet medicines based on gas chromatography-mass spectrometry

Contact Info

Product

Resources

About