Extraction, structural characterization, and immunobiological activity of ABP Ia polysaccharide from Agaricus bisporus

Guo, Liu; Ye, Jing; Li, Wei; Zhang, Jun; Wang, Qun; Zhu, Xiaoai; Miao, Jianyin; Huang, Yahui; Chen, Yunjiao; Cao, Yong

doi:10.1016/j.ijbiomac.2020.06.204

Cited by 28 publications

(17 citation statements)

References 55 publications

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“…Figure 1 presents the preliminary optimized results of the extraction conditions via single-factor experiments. As displayed in Figure 1 A, the cCPP yields significantly increased with an increasing liquid-to-material ratio from 10 to 30 mL/g, and then gradually stabilized, which is consistent with previous related research [ 27 ]. As reported, the improved liquid-to-material ratio could have been beneficial for polysaccharide dissolution, resulting in higher polysaccharide yields until reaching the saturated polysaccharide dissolution [ 28 ].…”

Section: Resultssupporting

confidence: 91%

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Jiao

et al. 2022

Nutrients

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In recent years, multiple edible polysaccharides from Codonopsis pilosula were mainly isolated with high average molecular weights and exhibited various bioactivities, but it was proven that low-molecular-weight polysaccharides could exert stronger activities due to the superior water solubility and permeability. In the present study, the water-soluble polysaccharide C. pilosula with low molecular weight was isolated under ultrasonic assistance at 30 °C, the extraction process was optimized via response surface method (RSM), and the structure and immunoregulatory activity were further investigated. The maximum yield (4.86%) for crude polysaccharides (cCPPs) was obtained under following parameters: ultrasonic power of 370 W, liquid/material ratio of 33 mL/g, ultrasonic time of 81 min. Subsequently, the cCPPs were further purified through dialysis and Sephadex G-25 column to acquire purified polysaccharide (CPPs). Structural analysis indicated that CPPs was a glucofructan (average molecular weight of 4.23 × 103 Da) with (2→1)-β-D-Fruf and (1→)-α-D-Glcp as the backbone branched by (2→6)-β-D-Fruf. Additionally, CPPs could enhance immunoregulatory function by stimulating NO production and cytokine (IL-6 and TNF-α) secretion of RAW264.7 macrophages dose-dependently, which presented no cytotoxic effects. These data suggest that CPPs have the potential to be used as a nutritional dietary compound and natural immunostimulant supplement in the food industry.

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Section: Resultssupporting

confidence: 91%

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Jiao

et al. 2022

Nutrients

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“…Results implied that three polysaccharides fractions mainly contained glucose and three fractions were an α-type, β-type, and β-type glycosidic linkages, respectively, suggesting that FVRP are more suitable to be used as functional factors in food, drugs, and cosmetic fields. Liu et al (2020) extracted Agaricus bisporus polysaccharides (ABP) using HWE and purified them by DEAE-52 and Sephadex G-200 column chromatography to obtain the homogeneous fraction (ABP Ia) with the Mw of 784 k D . Subsequently, the structural characteristics of ABP Ia were determined by GC–MS and FT–IR.…”

Section: Structural Characteristics Of Fungal Polysaccharidesmentioning

confidence: 99%

Polysaccharides from fungi: A review on their extraction, purification, structural features, and biological activities

Wang

Tan²,

Nima³

et al. 2022

Food Chemistry: X

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“…and the absorption peak located near 1,240 cm -1 might have resulted because of C-O-C vibration (Gao et al, 2020). Besides, a significant absorption peak was observed at 1,100 cm -1 in all samples, confirming the pyranose configuration of RPs (Liu et al, 2020). Meanwhile, the absorption peaks near 830 cm -1 and 760 cm -1 indicated the presence of α-or β-glycosidic bonds in RPs (Ognyanov et al, 2018).…”

Section: Fourier Transform Infrared Spectroscopy (Ft-ir)mentioning

confidence: 74%

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

Qiao

Cai

et al. 2021

J. Food Process. Preserv.

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The purpose of the present study is to illustrate the effects of extraction methods on the antioxidant activity and rheological properties of polysaccharides isolated from Ribes stenocarpum Maxim (RPs). RPs were extracted by ultrasonic-assisted extraction (RPs-1), alkaline extraction (RPs-2), acid extraction (RPs-3), microwaveassisted extraction (RPs-4), and three-phase separation (RPs-5). The results showed that RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 are acidic heteropolysaccharides with glucose, arabinose, and galacturonic acid as the main chain units and absence of triple-helix structure. Of them, RPs-3 possess the highest uronic acid content, best thermal stability, and strongest DPPH, ABTS, and hydroxyl radical scavenging abilities. Rheological properties showed that the above-mentioned polysaccharides are non-Newtonian fluids and own the similar shear thinning properties. RPs-3 represent the highest apparent viscosity and better stability in the over-acid or overalkali environment. The above results indicated that the acid extraction could serve as the most favorable extraction method for RPs. Novelty impact statement: A novel polysaccharide was isolated from Ribes stenocarpumMaxim by acid extraction, showing a stronger antioxidant activity and excellent rheological properties, which has a good application prospect in food processing.

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Extraction, structural characterization, and immunobiological activity of ABP Ia polysaccharide from Agaricus bisporus

Cited by 28 publications

References 55 publications

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Polysaccharides from fungi: A review on their extraction, purification, structural features, and biological activities

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

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