Extraction, Structural Characterization, and In Vivo Anti-Inflammatory Effect of Alginate from Cystoseira crinita (Desf.) Borry Harvested in the Bulgarian Black Sea

Kokova, Vesela; Lukova, Paolina; Baldzhieva, Alexandra; Katsarov, Plamen; Delattre, Cédric; Molinié, Roland; Petit, Elsa; Elboutachfaiti, Redouan; Murdjeva, Marianna; Apostolova, Elisaveta

doi:10.3390/md21040245

Cited by 7 publications

(3 citation statements)

References 67 publications

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“…d 5.05 ppm was assigned to the anomeric protons of the guluronic acid units (G), whereas the group of unresolved overlapping shifts at ca. d 4.7 ppm were tentatively assigned to the anomeric protons of the mannuronic acid units (M) and H5 protons of guluronic/mannuronic acid blocks [59,60]. However, the signal at ca.…”

Section: Band Maximum Position (Cmmentioning

confidence: 99%

Use of Limestone Sludge in the Preparation of ɩ-Carrageenan/Alginate-Based Films

Adão,

Calado,

Fernandes

et al. 2024

Materials

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The use of processed limestone sludge as a crosslinking agent for films based on Na–alginate and ɩ-carrageenan/Na-alginate blends was studied. Sorbitol was tested as a plasticizer. The produced gel formulations included alginate/sorbitol and carrageenan/alginate/sorbitol mixtures, with tested sorbitol concentrations of 0.0, 0.5 and 1.0 wt%. The limestone sludge waste obtained from the processing of quarried limestone was converted into an aqueous solution of Ca2+ by dissolution with mineral acid. This solution was then diluted in water and used to induce gel crosslinking. The necessity of using sorbitol as a component of the crosslinking solution was also assessed. The resulting films were characterized regarding their dimensional stability, microstructure, chemical structure, mechanical performance and antifungal properties. Alginate/sorbitol films displayed poor dimensional stability and were deemed not viable. Carrageenan/alginate/sorbitol films exhibited higher dimensional stability and smooth and flat surfaces, especially in compositions with 0.5 wt% sorbitol. However, an increasing amount of plasticizer appears to result in severe surface cracking, the development of a segregation phenomenon affecting carrageenan and an overall decrease in films’ mechanical resistance. Although further studies regarding film composition—including plasticizer fraction, film optimal thickness and film/mold material interaction—are mandatory, the attained results show the potential of the reported ɩ-carrageenan/alginate/sorbitol films to be used towards the development of viable films derived from algal polysaccharides.

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Section: Band Maximum Position (Cmmentioning

confidence: 99%

Use of Limestone Sludge in the Preparation of ɩ-Carrageenan/Alginate-Based Films

Adão,

Calado,

Fernandes

et al. 2024

Materials

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“…Sodium alginate (SA) (with molecular weights of 12,000-40,000 daltons) powder was obtained from Milipore Sigma (Sigma-Aldrich, St. Louis, MO, USA). This SA powder was extracted from Macrocystis pyrifera and composed of approximately 61% mannuronic and 39% guluronic acids [33]. The ratio of M/G acids is approximately 1.56.…”

Section: Experimental Procedures 211 Procurement Of Materialsmentioning

confidence: 99%

Effects of Sodium Alginate and Calcium Chloride on Fungal Growth and Viability in Biomass-Fungi Composite Materials Used for 3D Printing

Rahman,

Bedsole,

Akib

et al. 2024

Biomimetics

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To combat climate change, one approach is to manufacture products from biomass-fungi composite materials instead of petroleum-based plastics. These products can be used in packaging, furniture, and construction industries. A 3D printing-based manufacturing method was developed for these biomass-fungi composite materials, eliminating the need for molds, and enabling customized product design. However, previous studies on the 3D printing-based method showed significant shrinkage of printed samples. In this paper, an approach is proposed to reduce the shrinkage by incorporating ionic crosslinking into biomass-fungi composite materials. This paper reports two sets of experiments regarding the effects of sodium alginate (SA) and calcium chloride (CaCl2) on fungal growth and fungal viability. The first set of experiments was conducted using Petri dishes with fungi isolated from colonized biomass-fungi material and different concentrations of SA and CaCl2. Fungal growth was measured by the circumference of fungal colonies. The results showed that concentrations of SA and CaCl2 had significant effects on fungal growth and no fungal growth was observed on Petri dishes with 15% CaCl2. Some of these Petri dishes were also observed under confocal microscopy. The results confirmed the differences obtained by measuring the circumference of fungal colonies. The second set of experiments was conducted using Petri dishes with biomass-fungi mixtures that were treated with different concentrations of SA and exposure times in a CaCl2 (crosslinking) solution. Fungal viability was measured by counting colony-forming units. The results showed that the addition of the SA solution and exposure times in the crosslinking solution had statistically significant effects on fungal viability. The 2SA solution was prepared by dissolving 2 g of SA in 100 mL of water, the 5SA solution was prepared by dissolving 5 g of SA in 100 mL of water, and the crosslinking solution was prepared by dissolving 5 g of CaCl2 in 100 mL of water. The results also showed that fungal viability was not too low in biomass-fungi mixtures that included 2SA solution and were exposed to the crosslinking solution for 1 min.

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“…[28][29][30]. Alginates are also characterized by some medicinal properties, in particular, antitumor, immunomodulatory [31,32] and antioxidant [33] activities, antianaphylaxis [34], anti-inflammatory [35] effects, and exert antihypertensive potential [36]. The ability of alginates to sorption of heavy metals, metalloids, fluorides, and toxic compounds is used both in medicine and for the creation of bio-sorbents and materials for water purification [8][9][10][11][12][13]37,38].…”

Section: Introductionmentioning

confidence: 99%

Ions-Induced Alginate Gelation According to Elemental Analysis and a Combinatorial Approach

Zueva,

Khair,

Kazantseva

et al. 2023

IJMS

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This study considers the potential of elemental analysis of polysaccharide ionotropic gels in elucidating the junction zones for different divalent cations. The developed algorithm ensures the correct separation of contributions from physically adsorbed and structure-forming ionic compounds, with the obtained results scaled to alginate C12 block. Possible versions of chain association into dimers and their subsequent integration into flat junction zones were analyzed within the framework of the “egg-box” model. The application of combinatorial analysis made it possible to derive theoretical relations to find the probability of various types of egg-box cell occurrences for alginate chains with arbitrary monomeric units ratio μ = M/G, which makes it possible to compare experimental data for alginates of different origins. Based on literature data and obtained chemical formulas, the possible correspondence of concrete biopolymer cells to those most preferable for filling by alkaline earth cations was established. The identified features of elemental composition suggest the formation of composite hydrated complexes with the participation of transition metal cations. The possibility of quantitatively assessing ordered secondary structures formed due to the physical sorption of ions and molecules from environment, correlating with the sorption capabilities of Me2+ alginate, was established.

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Extraction, Structural Characterization, and In Vivo Anti-Inflammatory Effect of Alginate from Cystoseira crinita (Desf.) Borry Harvested in the Bulgarian Black Sea

Cited by 7 publications

References 67 publications

Use of Limestone Sludge in the Preparation of ɩ-Carrageenan/Alginate-Based Films

Use of Limestone Sludge in the Preparation of ɩ-Carrageenan/Alginate-Based Films

Effects of Sodium Alginate and Calcium Chloride on Fungal Growth and Viability in Biomass-Fungi Composite Materials Used for 3D Printing

Ions-Induced Alginate Gelation According to Elemental Analysis and a Combinatorial Approach

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