EXTRACTION STUDIES OF <i>PODOPHYLLUM HEXANDRUM</i> USING CONVENTIONAL AND NONCONVENTIONAL METHODS BY HPLC–UV–DAD

Gupta, D. K.; Verma, Mahendra K.; Lal, Shankar; Anand, Rajneesh; Khajuria, Rajni; Kitchlu, Surinder; Koul, Surrinder

doi:10.1080/10826076.2012.745134

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…To validate this method the amount of podophyllotoxin in the roots was determined by the traditional Soxhlet extraction method earlier described by Gupta and coworkers (Gupta et al 2013). Furthermore, the presence of podophyllotoxin was confirmed by LC-ESI-MS/MS.…”

Section: Resultsmentioning

confidence: 99%

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Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

et al. 2017

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Background and aim The endangered Podophyllum hexandrum is an important industrial source of podophyllotoxin, which is a precursor for the anticancer drugs etoposide and teniposide. Attempts to obtain podophyllotoxin through cell cultures or chemical synthesis have still a long way to go before being economical feasible. The objective of this study was to increase the root formation and podophyllotoxin production of P. hexandrum cultivated in a glasshouse. Methods Root formation and podophyllotoxin production of P. hexandrum in sand or peat-perlite soil at 15°C or 25°C was determined. Furthermore, the influence of methyl jasmonate on the podophyllotoxin production was determined. Results More root formation was observed in peat-perlite soil than in sand soil. Furthermore, root formation was higher at 15°C than at 25°C. This resulted in the highest podophyllotoxin production per plant in peat-perlite at 15°C (160 ± 22 mg/plant d.w.). Furthermore, methyl jasmonate treatment of the leaves increased the podophyllotoxin production in the roots by 21%. Conclusion We were able to cultivate P. hexandrum in a glasshouse in the Netherlands and improve the root formation and podophyllotoxin production. This paves the way for large-scale cultivation of P. hexandrum in the temperate latitudes for the production of the pharmaceutical interesting podophyllotoxin.

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Section: Resultsmentioning

confidence: 99%

“…The Soxhlet method has been previously used for podophyllotoxin extraction (Gupta et al 2013). Soxhlet extraction was done in a Tecator Soxtec System HT2 that consisted of two 1045 extraction units connected to one 1046 service unit (Gemini, Apeldoorn, The Netherlands).…”

Section: Soxhlet Extractionmentioning

confidence: 99%

Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

et al. 2017

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“…In many cases, these toxins are large polar compounds that cannot be extracted by supercritical fluids, but not always. Some examples are the isolation of toxins from Acorus calamus 18 or from Podophyllum hexandrum rizhomes 19 , where SFE provided much higher recoveries of some toxins, using neat CO 2 , than conventional Soxhlet.…”

Section: Removal Of Unwanted Compoundsmentioning

confidence: 99%

Membrane Separation

Voutsas¹

2014

Food Engineering Handbook

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Supercritical fluid extraction (SFE) has become one of the most popular green extraction techniques nowadays since it has demonstrated many advantages compared to traditional or classical extraction processes. Aspects such as improved selectivity, higher extraction yields, better fractionation capabilities and lower environmental impacts have been crucialto the important growth of SFE. In this chapter, fundamentals of SFE are presented together with the most important variables that can affect the extraction process and how to tune them. Moreover, interesting and new applications in different areas such as food science, pharmaceutical and others like, for instance, heavy metals recovery are presented.

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“…S. hexandrum embodies abundant pharmacological effects such as anti-proliferative, anti-viral, anti-in ammatory, anti-bacterial, insecticide and cytotoxic activities [2]. In recent decades, the main researches on S. hexandrum have been chie y focused on the establishment of chromatographic methods to quantitatively analyze and measure the content of podophyllotoxin, the chemical compositions and pharmacological effects, and how to prompt the sustainable development and utilization of this endangered plant [3][4][5]. So far, numerous chemical components have been isolated and identi ed from S. hexandrum, namely lignans, avonoids, saponins, polysaccharides and tannins, among which lignans are primarily composed of podophyllotoxin [3,6].…”

Section: Introductionmentioning

confidence: 99%

Potential Anti-Proliferative, Anti-Inflammatory and Anti-Viral Components from Sinopodophyllum Hexandrum Explored Using Bio-Affinity Ultrafiltration with Multiple Drug Targets

Li¹,

Chen²,

Zhang³

et al. 2021

Preprint

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Background: Sinopodophyllum hexandrum (S. hexandrum) is a typical Chinese herbal medicine with numerous components and remarkable pharmacological activities. However, the specific phytochemicals responsible for its anti-proliferative, anti-inflammatory and anti-viral effects remain unexplored.Methods: The integrated analytical strategy combining bio-affinity ultrafiltration with multiple drug targets was developed to rapidly screen and identify bioactive ligands from S. hexandrum. The in vitro anti-proliferative and COX-2 inhibitory assays of bioactive ligands screened were further verified by sulforhodamine B (SRB) cell proliferation and cytotoxicity detection and COX-2 inhibitor screening kits, respectively. Molecular docking analysis was also implemented by the AutoDockTools 1.5.6 software.Results: 10, 7, 9 and 9 phytochemicals were screened out and identified as the potential Topo I, Topo II, COX-2 and ACE2 ligands, respectively. Hereinto, podophyllotoxin and quercetin with higher EF values displayed strong inhibitory effects on A549 and HT-29 cells comparable with etoposide and 5-FU. Furthermore, compared with indomethacin at 0.73 ± 0.07 mM, podophyllotoxin and kaempferol with higher EF values exerted stronger inhibitory effects with IC50 values at 0.36 ± 0.02 mM and 10.49 ± 0.61 mM, respectively. Additionally, the optimal binding sites and mode of action between bioactive ligands and multiple drug targets were determined by molecular docking. Wherein, isorhamnetin showed a stronger affinity to ACE2 with the binding energy of -5.72 kcal/mol and the IC50 value at 63.95 mM, lower than MLN-4760 (-4.27 kcal/mol and 738.62 mM). Conclusions: The integrative strategy combining multiple drug targets and bio-affinity ultrafiltration LC-MS in the present study showed very promising potential for the quick screening and identifying bioactive ligands in S. hexandrum for Topo I, Topo II, COX-2 and ACE2, and some bioactive compounds screened out from this work were verified with other in vitro assays, and even better than those positive drugs of interest. Based on these findings, we then first constructed an interacting network among multi-components and multi-targets. In this way, we showcased a quick and reliable experimental strategy for uncovering the underlying mechanism of the empirical traditional applications of S. hexandrum which could also provide valuable information for better understanding the therapeutic targets and therapeutic ligands of other herbal medicines.

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EXTRACTION STUDIES OF PODOPHYLLUM HEXANDRUM USING CONVENTIONAL AND NONCONVENTIONAL METHODS BY HPLC–UV–DAD

Cited by 10 publications

References 22 publications

Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions

Membrane Separation

Potential Anti-Proliferative, Anti-Inflammatory and Anti-Viral Components from Sinopodophyllum Hexandrum Explored Using Bio-Affinity Ultrafiltration with Multiple Drug Targets

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