Extraction technology, component analysis, and in vitro antioxidant and antibacterial activities of total flavonoids and fatty acids from Tribulus terrestris L. fruits

Abstract

The current study focused on the extraction technology, components analysis, in vitro antioxidant and antibacterial activities of total flavonoids and fatty acids from Tribulus terrestris L. fruits. The extraction process of total flavonoids and fatty acids was optimized by the response surface method, and the compositions were identified from the two extracts by HPLC–DAD–ESI–MS− and GC–MS, respectively. In addition, the antioxidant and antibacterial activities were evaluated by assay of ABTS, DPPH radical scavenging activity, ferric reducing antioxidant power and minimal inhibitory concentration. The yields of total flavonoids and fatty acids were 0.46 and 9.76% under the optimized conditions. Moreover, nine and eight compositions were identified from the two extracts based on the related references, respectively. In addition, total flavonoids and fatty acids extracts both exhibited certain antioxidant and antibacterial activities. The present findings suggest that total flavonoids extracted from T. terrestris L. fruits comprised a more interesting candidate than fatty acids for the research and development of natural and healthy antioxidants and antibacterial agents for the pharmaceutical and food industries.

A Comprehensive Review of the Phytochemical, Pharmacological, and Toxicological Properties of Tribulus terrestris L.

“…These results suggest that the antibacterial activity of TT is correlated mostly with the saponin content. Flavonoid fractions from TT leaves and fruits have also been proven to have antibacterial activity against E. coli, Salmonella, Staphylococcus aureus, and Streptococcus [39,40].…”

Plants-Derived Biomolecules as Potent Antiviral Phytomedicines: New Insights on Ethnobotanical Evidences against Coronaviruses

“…P. tomentosa has wide-ranging biological activities comprising antiviral, antioxidant and antibacterial effects [ 136 , 138 ]. It is mostly used for the treatment of various diseases, such as inflammatory bronchitis, upper respiratory tract infection, asthma, tonsillitis, gonorrhea, traumatic bleeding, enteritis, bacteriological diarrhea, erysipelas, swelling, bronchopneumonia, conjunctivitis, and hemorrhoids [ 139 , 265 ]. Furthermore, P. tomentosa has been known to have an antiviral effect on SARS-CoV PLpro.…”

The Influence of Different Extraction Methods/Solvents on Composition, Biological Activities and ADMET Predictions of Phenolics in Tribulus terrestris

“…The antioxidant activities of the ethanol extract obtained from the fruit of the plant were found to have moderate DPPH (IC50: 11.52 µg /mL), ABTS (IC50: 6.74 µg /mL) radical scavenging and Brazilian Archives of Biology and Technology. Vol.64: e21210249, 2021 www.scielo.br/babt iron (III) ion reducing power activity (0.11 mmol Fe 2+ /100 µg/mL) compared to the standard [32]. In our present study, the DPPH radical scavenging activities of the aerial parts of the plant were examined and the IC50 values of the methanol extracts obtained by all three methods were found to be 0.277-0.372 mg/mL.…”

“…In another study, terrestrosin A-E, desgalactotigonin, F-gitonin, gitonin, desglucolanatigonin and (25R,S)-5α -spirostan-3βol -3 -O-β3-D-galactopyranosyl(1-2)-β-D-glucopyranosyl (1-4)-β-Dgalactopyranoside were isolated from an 80% ethanol extract prepared from the fruit of the plant [37]. It was determined by HPLC-DAD-ESI-MS that the ethanol extract obtained from the fruit of the plant contained quercetin 3,7 diglucoside+xyl, isomer of quercetin 3-O-arabinosyl galactoside, kaempferol-3-gentiobioside, isoquercitrin, astragaloside, sumaflavone, apigenin-6,8 glucoside+xyl, quercetin-3-gentiobioside [32]. According to our present knowledge, no studies have been reported regarding the effects of different extraction solvents/ methods on the chemical content of the plant.…”