Extractive ethoxycarbonylation in high-temperature gas chromatography–mass spectrometry based analysis of serum estrogens

Moon, Ju Yeon; Kang, Se Mi; Moon, Myeong Hee; Hong, Jongki; Kim, Ki Tae; Jeong, Dae Hoon; Kim, Young Nam; Chung, Bong Chul; Choi, Man Ho

doi:10.1016/j.jchromb.2011.10.024

Cited by 6 publications

(2 citation statements)

References 43 publications

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“…In previous studies, it successfully achieved good chromatographic properties for the analysis of lipid molecules including cholesterols ( Jung et al . 2009 , 2010 ), as well as estrogens with two-phase extractive EOC and subsequent PFP derivatization ( Moon et al . 2011 a , b ).…”

Section: Analytical Techniques For Sex Steroid Metabolomementioning

confidence: 99%

Metabolic profiling of cholesterol and sex steroid hormones to monitor urological diseases

Moon

Choi

Kim

2016

Endocrine-Related Cancer

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Cholesterol and sex steroid hormones including androgens and estrogens play a critical role in the development and progression of urological diseases such as prostate cancer. This disease remains the most commonly diagnosed malignant tumor in men and is the leading cause of death from different cancers. Attempts to understand the role of cholesterol and steroid metabolism in urological diseases have been ongoing for many years, but despite this, our mechanistic and translational understanding remains elusive. In order to further evaluate the problem, we have taken an interest in metabolomics; a discipline dedicated to the systematic study of biologically active metabolites in cells, tissues, hair and biofluids. Recently, we provided evidence that a quantitative measurement of cholesterol and sex steroid metabolites can be successfully achieved using hair of human and mouse models. The overall goal of this short review article is to introduce current metabolomic technologies for the quantitative biomarker assay development and also to provide new insight into understanding the underlying mechanisms that trigger the pathological condition. Furthermore, this review will place a particular emphasis on how to prepare biospecimens (e.g., hair fiber), quantify molecular profiles and assess their clinical significance in various urological diseases.

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Section: Analytical Techniques For Sex Steroid Metabolomementioning

confidence: 99%

Metabolic profiling of cholesterol and sex steroid hormones to monitor urological diseases

Moon

Choi

Kim

2016

Endocrine-Related Cancer

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“…Various chromatographic analytical methods have been reported for the determination of estrogens (Socas‐Rodriguez and others ), including high‐performance liquid chromatography (HPLC), gas chromatography (GC), liquid chromatography with mass spectrometry (LC‐MS; Vitku and others ; Wang and others b,c), and GC‐MS (Moon and others ). The preconcentration and clean‐up techniques for estrogens mainly included liquid–liquid extraction (Kuhnle and others ), dispersive liquid–liquid microextraction (Wu and others ), solid‐phase extraction (SPE; Sadowski and Gadzala‐Kopciuch ), micro‐SPE (μ‐SPE; Aftafa and others ), and solid‐phase micro‐extraction (SPME).…”

Section: Introductionmentioning

confidence: 99%

Determination of Estrogens in Milk Samples by Magnetic‐Solid‐Phase Extraction Technique Coupled With High‐Performance Liquid Chromatography

Wang

Cheng²,

Yang

2015

Journal of Food Science

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A magnetic-solid-phase extraction method coupled with high-performance liquid chromatography and diode array detection has been developed for simultaneous determination of 3 estrogens in milk samples. In this work, Fe3O4 NPs were synthesized by a simple chemical co-precipitation reaction, and the surface of Fe3O4 was modified with cetyltrimethyl ammonium bromide (CTAB; designed as Fe3O4@CTAB). The synthesized Fe3O4@CTAB NPs were characterized by Fourier transformed infrared spectroscopy, scanning electron microscopy, and transmission electron microscope. Fe3O4@CTAB NPs have high binding affinity toward estrone (E1), 17β-estradiol (17β-E2), and diethylstilbestrol (DES). Fe3O4@CTAB NPs can be easily separated from sample solutions using an external magnet due to the high super-paramagnetic property. The separation, preconcentration procedure is fast and will be completed in 2 min. Estrogens linear dynamic ranges were achieved in the range of 10 to 1000 ng/mL with regression coefficients (R(2)) higher than 0.9992. The limits of detection were between 0.26 and 0.61 ng/mL. Parameters influencing the recoveries were investigated and optimized. The proposed method was used for the determination of E1, 17β-E2, and DES in milk samples, and recoveries were ranged from 91.3% to 105.0%, with the relative standard deviations in the range of 2.7% to 4.0%.

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Improved detectability of sex steroids from frozen sections of breast cancer tissue using GC-triple quadrupole-MS

Moon

McNamara

Lee

et al. 2018

The Journal of Steroid Biochemistry and Molecular Biology

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Extractive ethoxycarbonylation in high-temperature gas chromatography–mass spectrometry based analysis of serum estrogens

Cited by 6 publications

References 43 publications

Metabolic profiling of cholesterol and sex steroid hormones to monitor urological diseases

Metabolic profiling of cholesterol and sex steroid hormones to monitor urological diseases

Determination of Estrogens in Milk Samples by Magnetic‐Solid‐Phase Extraction Technique Coupled With High‐Performance Liquid Chromatography

Improved detectability of sex steroids from frozen sections of breast cancer tissue using GC-triple quadrupole-MS

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