Extractive Fermentation Processes: Modes of Operation and Application

Teke, George M.; Tai, Siew Leng; Pott, Robert William McClelland

doi:10.1002/cben.202100028

Cited by 19 publications

(6 citation statements)

References 176 publications

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“…Inspired by the above results about the higher reaction rates with furfural (Figure C), it is envisioned that the catalytic efficiencies of whole cells may be greatly increased by improving mass transfer (e.g., increasing cell membrane permeability). In extractive fermentation, hydrophobic solvents are usually added to improve the cell membrane permeability as well as to in situ recover products . Considering the fact that acetate esters are used as acyl donors in the following BHMF diacetate synthesis, the intensification of biocatalytic BHMF synthesis was performed by using acetate esters (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…In extractive fermentation, hydrophobic solvents are usually added to improve the cell membrane permeability as well as to in situ recover products. 64 Considering the fact that acetate esters are used as acyl donors in the following BHMF diacetate synthesis, the intensification of biocatalytic BHMF synthesis was performed by using acetate esters (Figure 3). Figure 3A shows the effect of EtOAc contents on the HMF reduction.…”

Section: Table 1 Apparent Kinetic Parameters Of Adhs Toward Hmf and N...mentioning

confidence: 99%

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One-Pot Chemobiocatalytic Production of 2,5-Bis(hydroxymethyl)furan and Its Diester from Biomass in Aqueous Media

Qian

Zong

2023

ACS Catal.

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Direct valorization of biomass into value-added chemicals is attractive yet challenging. Herein, we report a one-pot synthesis of 2,5-bis(hydroxymethyl)furan (BHMF) and its diacetate from inexpensive fructose and sugar syrup in the aqueous phase via sequential chemical dehydration, biocatalytic reduction, and esterification. A robust alcohol dehydrogenase from Escherichia coli (EcYjgB) was identified for HMF reduction, with high catalytic efficiencies toward 5hydroxymethylfurfural (HMF) (k cat /K m , 1300 s −1 mM −1 ) as well as NADPH (9700 s −1 mM −1 ). Recombinant E. coli harboring EcYjgB (E. coli_EcYjgB) showed high tolerance toward highly toxic and strongly inhibitory biobased furans, enabling smooth reduction of 1 M HMF and 0.5 M furfural. Its practicality was demonstrated by gram-scale synthesis of BHMF with a 79% isolated yield at approximately 126 g/L substrate loading. Biocatalytic process intensification was realized by improving cell membrane permeability using ethyl acetate, providing up to 15 g/L h BHMF productivity. A concurrent biocatalytic cascade for producing BHMF diacetate from HMF was constructed by combining E. coli_EcYjgB and Mycobacterium smegmatis acyltransferase (MsAcT) variant N94A, affording the desired product with a 93% yield in 1 h. Starting from crude HMF obtained via chemical dehydration and organic solvent evaporation, BHMF and its diacetate were produced with >99 and 88% yields, respectively. Finally, direct synthesis of these furan-based products from sugars was performed by chemobiocatalysis, without isolation of any intermediates, resulting in yields of up to 59% (relative to sugar). This work may pave the way for scalable production of furan-based chemicals from inexpensive renewable biomass.

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Section: Resultsmentioning

confidence: 99%

Section: Table 1 Apparent Kinetic Parameters Of Adhs Toward Hmf and N...mentioning

confidence: 99%

One-Pot Chemobiocatalytic Production of 2,5-Bis(hydroxymethyl)furan and Its Diester from Biomass in Aqueous Media

Qian

Zong

2023

ACS Catal.

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“…A study by (Nascimento et al, 2023) methods can be challenging due to the interference of biomass with downstream separation processes-biomass will also be collected along with precipitated MELs. Consequently, additional cell separation techniques may be required before implementing an in situ precipitation process (Teke, Tai, et al, 2022). In the context of the study by Kitamoto, Akiba, et al (1990), observed coprecipitation of MELs with yeast cells and oil at reduced pH levels may be linked to their association with other components like proteins, oil, and FFA in the fermentation broth, rather than a direct response to pH changes.…”

Section: Membrane Filtrationmentioning

confidence: 99%

A review on the upstream production and downstream purification of mannosylerythritol lipids

Valkenburg,

Ncube,

Teke

et al. 2023

Biotech & Bioengineering

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Biosurfactants are natural compounds with remarkable surface‐active properties that may offer an eco‐friendly alternative to conventional surfactants. Among them, mannosylerythritol lipids (MELs) stand out as an intriguing example of a glycolipid biosurfactant. MELs have been used in a variety of sectors for various applications, and are currently commercially produced. Industrially, they are used in the pharmaceutical, cosmetic, food, and agricultural industries, based on their ability to reduce surface tension and enhance emulsification. However, despite their utility, their production is comparatively limited industrially. From a bioprocessing standpoint, two areas of interest to improve the production process are upstream production and downstream (separation and purification) product recovery. The former has seen a significant amount of research, with researchers investigating several production factors: the microbial species or strain employed, the producing media composition, and the production strategy implemented. Improvement and optimization of these are key to scale‐up the production of MELs. On the other hand, the latter has seen comparatively limited work presented in the literature. For the most part traditional separation techniques have been employed. This systematic review presents the production and purification methodologies used by researchers by comprehensively analyzing the current state‐of‐the‐art with regards the production, separation, and purification of MELs. By doing so, the review presents different possible approaches, and highlights some potential areas for future work by identifying opportunities for the commercialization of MELs.

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“…Due to product toxicity and possible product degradation, VFA extraction steps were strategized to selectively recover (and potentially concentrate) the targeted VFAs and achieve simultaneous impurity and contaminant removal. Numerous methods have been employed to extract VFAs from the aqueous phase, including electrodialysis, adsorption, and liquid-liquid extraction (LLE) [32]. It is noted that the extraction steps are often followed by a secondary purification stage, using technologies including distillation, evaporation, and crystallization, to reach the targeted purity for potential sale in the market or downstream upgrading [33].…”

Section: Extraction Of Vfasmentioning

confidence: 99%

“…The liquid used as the extracting solvent is commonly called the extractant, while the other liquid phase is known as the raffinate. During LLE, VFAs are partitioned from the digestate (raffinate phase) to the solvent phase, and the partition coefficient relies on multiple factors, such as digestate pH, initial VFA concentration, and any other solutes in the digestate [32]. However, solvent selection is particularly important for extraction performance.…”

Section: Liquid-liquid Extraction (Lle)mentioning

confidence: 99%

Volatile Fatty Acid Recovery from Arrested Anaerobic Digestion for the Production of Sustainable Aviation Fuel: A Review

Ge,

Chen,

Sànchez i Nogué

et al. 2023

Fermentation

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The process of producing sustainable aviation fuel (SAF) from organic waste involves the use of volatile fatty acids (VFAs) as intermediates that are obtained via arrested anaerobic digestion (AAD) and VFA recovery. This recovery process often requires several steps, including dewatering, filtration, extraction, and purification. The recovery of VFAs is crucial for their upgrading and can pose a challenge in the production of SAF from organic waste due to high costs and compatibility issues. This review discusses various dewatering methods, including centrifuges, belt filter presses, and screw presses, and explores conditioning technologies that can improve dewatering performance. It also introduces filtration technologies, with a focus on dynamic filtration, which shows promise in addressing the issue of membrane fouling. Additionally, the review describes extraction technologies such as electrodialysis, adsorption, and liquid–liquid extraction (LLE). By providing insights into these different techniques, the review aims to contribute to the development of an integrated VFA recovery process with low carbon footprint.

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Extractive Fermentation Processes: Modes of Operation and Application

Cited by 19 publications

References 176 publications

One-Pot Chemobiocatalytic Production of 2,5-Bis(hydroxymethyl)furan and Its Diester from Biomass in Aqueous Media

One-Pot Chemobiocatalytic Production of 2,5-Bis(hydroxymethyl)furan and Its Diester from Biomass in Aqueous Media

A review on the upstream production and downstream purification of mannosylerythritol lipids

Volatile Fatty Acid Recovery from Arrested Anaerobic Digestion for the Production of Sustainable Aviation Fuel: A Review

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