Extracts from Black Carrot Tissue Culture as Potent Anticancer Agents

Abstract

Black carrots contain anthocyanins possessing enhanced physiological activities. Explants of young black carrot shoots were cultured in Murashige and Skoog (MS) medium for callus initiation and were transferred to new MS medium supplemented with four different combinations of 2,4-dichlorophenoxyacetic acid and kinetin. Subsequently, the lyophilized calli and black carrot harvested from fields were subjected to ultrasound extraction with ethanol at a ratio of 1:15 (w:v). Obtained extracts were applied to various human cancer cell lines including MCF-7 SK-BR-3 and MDA-MB-231 (human breast adenocarcinomas), HT-29 (human colon adenocarcinoma), PC-3 (human prostate adenocarcinoma), Neuro 2A (Musmusculus neuroblastoma) cancer cell lines and VERO (African green monkey kidney) normal cell line by MTT assay. The highest cytotoxic activity was achieved against Neuro-2A cell lines exhibiting viability of 38-46% at 6.25 μg/ml concentration for all calli and natural extracts. However, a significantly high IC50 value of 170.13 μg/ml was attained in normal cell line VERO indicating that its natural counterpart is an ideal candidate for treatment of brain cancer without causing negative effects to normal healthy cells.

Black carrot pomace as a source of polyphenols for enhancing the nutritional value of cake: An in vitro digestion study with a standardized static model

“…Recently, they have received increasing interest as a source of natural food colorant due to the legal restrictions for synthetic colorants and increasing consumer demand for natural pigments (Ekici et al, 2015). In addition to their colorant features, anthocyanin rich black carrots are indicated to exhibit health promoting properties including antioxidant (Kamiloglu, Pasli, Ozcelik, Van Camp, & Capanoglu, 2015;Koley et al, 2014), antiinflammatory (Kamiloglu et al, 2016b;Mizgier et al, 2016) and anticancer (Sevimli-Gur, Cetin, Akay, Gulce-Iz, & Yesil-Celiktas, 2013) activities.…”

Is carrot consumption associated with a decreased risk of lung cancer? A meta-analysis of observational studies

“…Carrot is the main source of α-carotene, and previous studies have shown that carrot may play an important role in the prevention of cancers (4,5,6) . The association between carrot consumption and lung cancer has drawn much attention since the 1980s.…”

Essential oils in seeds of Daucus spp.: Insights into the development of potential new cultivars

“…Comparative cytology and mapping studies are valuable resources for in-depth genetic and genomic research of Daucus spp., and for assisting in breeding programs Iovene et al, 2011). Plant tissue culture and genetic transformation have been also utilized in enhancing secondary metabolites production (Aharoni et al, 2005;Gonç alves and Romano, 2013;Govindaraj et al, 2008;Hasunuma et al, 2008;Mendoza-Poudereux et al, 2014;Muñoz-Bertomeu et al, 2008;Sevimli-Gur et al, 2013).…”

“…In addition to their nutritional values, carrots are used in many medical applications and are known for their therapeutic properties resulting from the antibacterial, antifungal and antitumor activities of their essential oils (Glišić et al, 2007;Sevimli-Gur et al, 2013;Shebaby et al, 2014;Tavares et al, 2008), and their ethno-medicinal properties in wound healing activity (Patil et al, 2012). It is reported that the volatile oil from carrot seeds possess analgesic, anti-inflammatory and antibacterial activity (Radulović et al, 2011).…”