Extracts from Calendula officinalis Offer in Vitro Protection Against H₂O₂ Induced Oxidative Stress Cell Killing of Human Skin Cells

Abstract

The in vitro safety and antioxidant potential of Calendula officinalis flower head extracts was investigated. The effect of different concentrations (0.125, 0.5, 1.0, 2.0 and 5.0% (v/v)) of Calendula extracts on human skin cells HaCaT in vitro was explored. Doses of 1.0% (v/v) (0.88 mg dry weight/mL) or less showed no toxicity. Cells were also exposed to the Calendula extracts for either 4, 24 or 48 h before being exposed to an oxidative insult (hydrogen peroxide H2 O2 ) for 1 h. Using the MTT cytotoxicity assay, it was observed that two independent extracts of C. officinalis gave time-dependent and concentration-dependent H2 O2 protection against induced oxidative stress in vitro using human skin cells. Pre-incubation with the Calendula extracts for 24 and 48 h increased survival relative to the population without extract by 20% and 40% respectively following oxidative challenge. The antioxidant potential of the Calendula extracts was confirmed using a complimentary chemical technique, the DPPH(●) assay. Calendula extracts exhibited free radical scavenging abilities. This study demonstrates that Calendula flower extracts contain bioactive and free radical scavenging compounds that significantly protect against oxidative stress in a human skin cell culture model.

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“…Cell viability was then tested via MTT assay. The MTT assay showed that cell viability of Vero cells was significantly decreased after treatment officinalis flower extracts contain bioactive and free radical scavenging compounds that significantly protect against oxidative stress in a human skin cell culture model [55].…”

Calendula officinalis Protection Against Cytotoxicity Effects of Personal Care Products on HaCaT Human Skin Cells

“…Therefore, HaCaT cells treated with Calendula extract C to produce bioactivity antioxidant compounds did protect against toxicity of personal care products (beauty products and head lice treatments) that caused oxidative stress cell killing. The protection offered by Calendula extract C was time-dependent as explored early [4,5]. It was noted that there was significantly higher protection after pre-treatment with calendula extract with compounds that displayed higher toxicity when induced by the treatment of compounds alone.…”

“…The toxicity results of personal care products on HaCaT cells were measured by MTT assay which is a relative survival assay. Calendula officinalis showed protection activity against hydrogen peroxide (H 2 O 2 ) induced oxidative stress cell killing and chromosomal damage [4,5]. Therefore, HaCaT cells treated with Calendula extract C to produce bioactivity antioxidant compounds did protect against toxicity of personal care products (beauty products and head lice treatments) that caused oxidative stress cell killing.…”

“…The characterization of calendula extract C and its ability to protect human skin cells HaCaT against hydrogen peroxide (H 2 O 2 ) induced oxidative stress cell killing and genetic damage was published previously [4,5]. Briefly, dried flowers of Calendula officinalis were supplied by an industry partner (Jurlique International, Mount Barker, South Australia) from biodynamically grown plants.…”

“…Oxidative stress interacts with the process of variety of skin diseases [1]. Calendula flower has been shown to protect against cells being killed and chromosomal damage induced by hydrogen peroxide (H 2 O 2 ) in HaCaT human skin cells [4,5]. It was shown that Calendula officinalis contains a number of compounds with antioxidants and free radicals with scavenging potential.…”