Extracts from the Roots ofLindera strychifoliaInduces Apoptosis in Lung Cancer Cells and Prolongs Survival of Tumor-bearing Mice

Abstract

Lindera strychifolia, a scandent shrub Lauraceous medicinal plant, has been used in Chinese traditional medicine as a palliative and an anti-spasmodic. It also shows cytotoxic effects against several tumor cell lines and inhibits marcromolecule biosynthesis. This study investigated the anti-tumor effects of L. strychifolia extract against lung cancer cells using in vitro and in vivo models. Two human lung cancer cell lines A549 (adenocarcinoma) and SBC-3 (small cell carcinoma), and a non-tumor cell line 3T3-L1 (mice fibroblasts) were subjected to L. strychifolia extract treatment. On lung cancer cells, L. strychifolia induced cell growth inhibition in a dose-dependent manner. Conversely, the extract did not show any significant cytotoxic effect on 3T3-L1 cells. Therefore, the extract is specific for tumor cells. Tumor cells treated with L. strychifolia extract showed typical morphological appearance of apoptosis including nuclei fragmentation and cell condensation. The in vivo effects of L. strychifolia extract were investigated in C57BL/6 mice transplanted with Lewis lung cancer (LL-2) cells, and in BALB/c nude mice transplanted with A549 or SBC-3 human lung cancer cells. Oral administration of L. strychifolia extract prolonged survival time and inhibited tumor growth in a dose-dependent manner by inducing apoptosis in the LL-2 cell mice model. Furthermore, in A549 or SBC-3 cell nude mice models, oral administration of L. strychifolia extract also significantly inhibited tumor growth at the 5.0 mg/ml concentration. These findings suggested that the components of L. strychifolia have anticancer activity and may contribute to clinical applications in the prevention and treatment of lung cancer.

Review on Functions of Dietary Agents and Plants in Cancer Prevention and Treatment

“…Increase oral bioavailability of anticancer agents [95] Lindera strychifolia Roots Extract Induces apoptosis A-549 and SBC-3 human lung cancer cell line [96] (Continued) Cell cycle arrest in G0/G1 phase as well as decrease of cells in S -phase Human colorectal adenocarcinoma. [134] plagued by lung, colon, rectum and prostrate cancer.…”

Potential agents for cancer and obesity treatment with herbal medicines from the green garden

“…1. Fujiwara and co-workers have studied the inhibitory effects of extracts of Lindera strychifolia, which can induce inhibition of cell growth in a dose-dependent manner in human lung cancer, and did not show any significant cytotoxicity [27]. Additionally, L. strychifolia extracts significantly inhibited tumor growth at a concentration of 5 mg/mL in a mouse model.…”

Antioxidant Activities of Uyaku (Lindera Strychnifolia) Leaf Extract: A Natural Extract Used in Traditional Medicine

“…The well-known Uyaku compounds mentioned above, and other sesquiterpenes such as isolinderalactone, neolinderalactone, lindestrenolide lindenone, linderazulene, chamazulene, laurolitsine, isogermafurene have been summarized in previous other studies [2][3][4][5]. Recent studies demonstrated that Uyaku extract has antiviral activity [21], an inhibitory effect on prolyl endopeptidase [22], and is associated with anticancer activity in lungs [23]. It has also a potentially protective effect against post-ischemic myocardial dysfunction [24], and diabetic nephropathy [25].…”