Extracts of Phyllostachys pubescens Leaves Represses Human Steroid 5-Alpha Reductase Type 2 Promoter Activity in BHP-1 Cells and Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rat Model

Abstract

Benign prostatic hyperplasia (BPH) is the most common symptomatic abnormality of the human prostate characterized by uncontrolled proliferation of the prostate gland. In this study, we investigated the effect of bamboo, Phyllostachys pubescens, leaves extract (PPE) on human 5α-reductase type 2 (SRD5A2) gene promoter activity in human prostate cell lines and the protective effect of PPE on a testosterone-induced BPH rat model. PPE repressed human SRD5A2 promoter activity and its mRNA expression. The rats treated with PPE for 4 weeks showed a significantly attenuated prostate weight compared to vehicle control. PPE-treated rats also showed reduced serum dihydrotestosterone, testosterone, prostate-specific antigen, and SRD5A2 levels by testosterone injection. Quantitative real-time polymerase chain reaction showed that PPE treatment significantly decreased mRNA expression of SRD5A2, androgen receptor (AR), proliferating cell nuclear antigen (PCNA), and fibroblast growth factor 2 compared with the vehicle-treated, testosterone-injected rats in the prostate. Furthermore, PPE treatment showed reduced AR, PCNA, and tumor necrosis factor alpha expression in the prostate via immunohistofluorescence staining. In conclusion, oral administration of PPE prevented and inhibited the development and progression of enlarged prostate lesions in testosterone-induced animal models through various anti-proliferative and anti-inflammatory pharmacological effects and induced suppression of SRD5A2 gene expression.

Effects of Bioactive Composition in Oryza sativa L. cv. KDML105 Bran Extract on Gene Expression Related to Hair Cycle in Human Hair Follicle Dermal Papilla Cells

“…The γ-tocopherol content (Figure 1) was highest, followed by α-tocopherol, β-tocopherol, and δ-tocopherol. Finasteride and dutasteride, 5α-reductase inhibitors, suppressed SRD5A expression in previous studies [31,32]. Purmorphamine, an agonist of the smoothened receptor, has been reported to enhance the gene expression of mediators in the sonic hedgehog pathway [33,34].…”

Effects of Alginate Oligosaccharide on Testosterone-Induced Benign Prostatic Hyperplasia in Orchiectomized Rats

“…Human prostate epithelial (BPH-1) cells were cultured and maintained as previously described [ 33 ]. Briefly, cells were maintained in RPMI 1640 (Thermo Scientific, Waltham, MA, USA) supplemented with 20% fetal bovine serum (FBS).…”

Phytochemical Characterization for Quality Control of Phyllostachys pubescens Leaves Using High-Performance Liquid Chromatography Coupled with Diode Array Detector and Tandem Mass Detector

“…The system was controlled by LabSolution software (version 5.53, SP3, Kyoto, Japan). Analytical conditions such as analytical column, mobile phase, and gradient elution conditions for simultaneous quantification of marker components were determined according to a previous protocol [ 15 ]. The markers were quantified by measuring absorbance at 310 nm for p -coumaric acid, 325 nm for chlorogenic acid, 335 nm for isovitexin, and 350 nm for isoorientin and orientin using a DAD that simultaneously scanned from 190 to 400 nm.…”

“…Vinpocetine, isolated from leaves of P. pubescens , has also been reported to prevent osteoblast apoptosis and osteonecrosis of the femoral head [ 14 ]. We recently investigated the effect of extracts from P. pubescens leaves on SRD5A2 gene expression in human prostate cell lines and an animal model of testosterone-induced benign prostatic hyperplasia [ 15 ].…”