Extracts of Stevia rebaudiana against Fusarium oxysporum associated with tomato cultivation

Ramírez, Priscila Guerra; Ramírez, Diana Guerra; Mejía, Emma Zavaleta; Ocampo, Sergio Aranda; Díaz, Cristian Nava; Rojas-Martínez, Reyna Isabel

doi:10.1016/j.scienta.2019.108683

Cited by 18 publications

(14 citation statements)

References 22 publications

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“…Since -to the best of the authors' knowledge-this is the first time that this compound is assayed against Botryosphaeriaceae fungi, no comparisons with similar taxa in terms of MIC values are available. However, the detected antifungal activity would be in good agreement with the results presented by other authors, who reported an inhibitory effect against other fungi (Alternaria solani Sorauer, Helminthosporium solani Durieu & Montagne, Aspergillus spp., Fusarium spp., Penicillium chrysogenum Thom, or Botrytis cinerea Pers., among others) [56][57][58][59][60], with MIC values varying over a wide range (from 250 to 3000 μg•mL −1 ).…”

Section: Efficacy Of the Treatmentsmentioning

confidence: 99%

Antifungal Activity against Botryosphaeriaceae Fungi of the Hydro-Methanolic Extract of Silybum marianum Capitula Conjugated with Stevioside

Langa-Lomba

Buzón-Durán

Sánchez-Hernández

et al. 2021

Plants

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Silybum marianum (L.) Gaertn, viz. milk thistle, has been the focus of research efforts in the past few years, albeit almost exclusively restricted to the medicinal properties of its fruits (achenes). Given that other milk thistle plant organs and tissues have been scarcely investigated for the presence of bioactive compounds, in this study, we present a phytochemical analysis of the extracts of S. marianum capitula during the flowering phenological stage (stage 67). Gas chromatography–mass spectroscopy results evidenced the presence of high contents of coniferyl alcohol (47.4%), and secondarily of ferulic acid ester, opening a new valorization strategy of this plant based on the former high-added-value component. Moreover, the application of the hydro-methanolic extracts as an antifungal agent has been also explored. Specifically, their activity against three fungal species responsible for the so-called Botryosphaeria dieback of grapevine (Neofusicoccum parvum, Dothiorella viticola and Diplodia seriata) has been assayed both in vitro and in vivo. From the mycelial growth inhibition assays, the best results (EC90 values of 303, 366, and 355 μg·mL−1 for N. parvum, D. viticola, and D. seriata, respectively) were not obtained for the hydroalcoholic extract alone, but after its conjugation with stevioside, which resulted in a strong synergistic behavior. Greenhouse experiments confirmed the efficacy of the conjugated complexes, pointing to the potential of the combination of milk thistle extracts with stevioside as a promising plant protection product in organic Viticulture.

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Section: Efficacy Of the Treatmentsmentioning

confidence: 99%

Antifungal Activity against Botryosphaeriaceae Fungi of the Hydro-Methanolic Extract of Silybum marianum Capitula Conjugated with Stevioside

Langa-Lomba

Buzón-Durán

Sánchez-Hernández

et al. 2021

Plants

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“…These authors found a MIC of 2 mg·mL −1 for F. oxysporum , much higher than the one reported herein. Ramírez et al [ 16 ] evaluated the antifungal activity of extracts of different polarity obtained from S. rebaudiana leaves against F. oxysporum as well, finding that the hexane extract at a concentration of 833 ppm (five times higher than the one reported herein for stevioside) only inhibited mycelial growth by around 50%. The differences may be ascribed to both the sensitivity of the pathogen under study and the use of stevia leaf extract instead of the purified stevioside.…”

Section: Discussionmentioning

confidence: 89%

“…Nonetheless, a literature survey revealed that their antifungal/fungistatic activity has been little studied [ 11 , 12 , 13 , 14 ]. With regard to phytopathogenic fungi, promising results were reported by Shukla et al [ 15 ] and Ramírez et al [ 16 ] against Fusarium oxysporum .…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activity against Fusarium culmorum of Stevioside, Silybum marianum Seed Extracts, and Their Conjugate Complexes

et al. 2020

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Fusarium head blight (FHB) is a disease that poses a major challenge in cereal production that has important food and feed safety implications due to trichothecene contamination. In this study, the effect of stevioside—a glycoside found in the leaves of candyleaf (Stevia rebaudiana Bertoni)—was evaluated in vitro against Fusarium culmorum (W.G. Smith) Sacc., alone and in combination (in a 1:1 molar ratio) with polyphenols obtained from milk thistle seeds (Silybum marianum (L.) Gaertn). Different concentrations, ranging from 32 to 512 µg·mL−1, were assayed, finding EC50 and EC90 inhibitory concentrations of 156 and 221 µg·mL−1, respectively, for the treatment based only on stevioside, and EC50 and EC90 values of 123 and 160 µg·mL−1, respectively, for the treatment based on the stevioside–polyphenol conjugate complexes. Colony formation inhibition results were consistent, reaching full inhibition at 256 µg·mL−1. Given that synergistic behavior was observed for this latter formulation (SF = 1.43, according to Wadley’s method), it was further assessed for grain protection at storage, mostly directed against mycotoxin contamination caused by the aforementioned phytopathogen, confirming that it could inhibit fungal growth and avoid trichothecene contamination. Moreover, seed tests showed that the treatment did not affect the percentage of germination, and it resulted in a lower incidence of root rot caused by the pathogen in Kamut and winter wheat seedlings. Hence, the application of these stevioside–S. marianum seed extract conjugate complexes may be put forward as a promising and environmentally friendly treatment for the protection of cereal crops and stored grain against FHB.

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“…In the same context, previously major components of S. rebaudiana's essential oil [13,14], such as spathulenol, caryophyllene oxide, manool oxide, trans-nerolinol, and α-cadinol are constituents of numerous natural products, with pronounced biological activities. Recently, plant protection properties of S. rebaudiana have been published against the phytopathogenic fungi Fusarium oxysporum [15,16] in the frame of developing safe and effective strategies, but alternative to the synthetic, plant protection products. In recent years there is an uprising global need for ecofriendly substitutes to the synthetic pesticides since many chemical groups of formulates have been associated with ecotoxicity concerns and, as a result, have been withdrawn from the market [17,18].…”

Section: Soil Amending With S Rebaudiana Leaves Powder (Lp) and Wood Powder (Wp) To Treat Against M Incognita And Subsequent Biofertilizamentioning

confidence: 99%

Nematicidal Activity of Stevia rebaudiana (Bertoni) Assisted by Phytochemical Analysis

Ntalli

Kasiotis

Baira

et al. 2020

Toxins

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To date, there has been great demand for ecofriendly nematicides with beneficial properties to the nematode hosting plants. Great efforts are made towards the chemical characterization of botanical extracts exhibiting nematicidal activity against Meloidogyne spp., but only a small percentage of these data are actually used by the chemical industry in order to develop new formulates. On the other hand, the ready to use farmer produced water extracts based on edible plants could be a sustainable and economic solution for low income countries. Herein, we evaluate the nematicidal potential of Stevia rebaudiana grown in Greece against Meloidogyne incognita and Meloidogyne javanica, two most notorious phytoparasitic nematode species causing great losses in tomato cultivation worldwide. In an effort to recycle the plant’s remnants, after leaves selection for commercial use, we use both leaves and wooden stems to test for activity. In vitro tests demonstrate significant paralysis activity of both plant parts’ water extracts against the second-stage juvenile (J2) of the parasites; while, in vivo bioassays demonstrated the substantial efficacy of leaves’ powder (95% at 1 g kg−1) followed by stems. Interestingly, the incorporation of up to 50 g powder/kg of soil is not phytotoxic, which demonstrates the ability to elevate the applied concentration of the nematicidal stevia powder under high inoculum level. Last but not least, the chemical composition analyses using cutting edge analytical methodologies, demonstrated amongst components molecules of already proven nematicidal activity, was exemplified by several flavonoids and essential oil components. Interestingly, and to our knowledge, for the flavonoids, morin and robinin, the anthocyanidin, keracyanin, and a napthalen-2-ol derivative is their first report in Stevia species.

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Extracts of Stevia rebaudiana against Fusarium oxysporum associated with tomato cultivation

Cited by 18 publications

References 22 publications

Antifungal Activity against Botryosphaeriaceae Fungi of the Hydro-Methanolic Extract of Silybum marianum Capitula Conjugated with Stevioside

Antifungal Activity against Botryosphaeriaceae Fungi of the Hydro-Methanolic Extract of Silybum marianum Capitula Conjugated with Stevioside

Antifungal Activity against Fusarium culmorum of Stevioside, Silybum marianum Seed Extracts, and Their Conjugate Complexes

Nematicidal Activity of Stevia rebaudiana (Bertoni) Assisted by Phytochemical Analysis

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