Abstracts Glaucousness is a visual trait related to the colour of the photosynthetic surface and hence it can be easily selected. It is associated both with the deposition and orientation of wax platelets on the cuticle of the photosynthetic surface. It is known that the glaucous leaf character is determined by the W1 gene, and Iw1 and Iw1 DIC genes from Triticum dicoccoides, which act as an epistatic inhibitor to a glaucousness. The aim of the present study was to map W1, Iw1 and Iw1 DIC from T. dicoccoides in the short arm of chromosome 2B of tetraploid wheat. Segregation of F 2 populations of three hybrids indicated that the marker Xgwm455 is linked with and distal to Iw1 (16.7 cM), two markers are tightly linked with W1, and Iw1 DIC is linked with Xgwm614 and Xwmc661 distally located on chromosome 2BS. From three derived maps, it is suggested that Iw1 DIC locus is different from W1. We discuss the difference between Vir and Iw1 DIC from T. dicoccoides.