Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

Schmidt, Hans C.; Hagens, Johanna; Schuppert, Pauline; Philippi, Clara; Reinshagen, Konrad; Tomuschat, Christian

doi:10.3791/64424

Cited by 2 publications

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“…Cutting of pyloric region of the stomach and duodenum right lateral of the duodenal papilla was performed. Remaining structures connecting the liver to the abdomen were dissected and the En-Bloc-sample was transferred to a foam mat where the liver and EHBD samples were isolated [130]. Tissue samples were immediately xated in Paraformaldehyde (4% PFA in PBS) after harvesting.…”

Section: Techniquesmentioning

confidence: 99%

Biliatresone and Biliary atresia – Disease modelling in C57BL/6J neonates

Schmidt

Hagens

Schuppert

et al. 2023

Preprint

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Exposure to plant toxins or microbiota that are able to digest common food ingredients to toxic structures might be responsible for biliary atresia (BA). An isoflavonoid, biliatresone is known to effectively alter the extrahepatic bile duct (EHBD) development in BALB/c mice. Biliatresone causes a reduction of Glutathione (GSH) levels, SOX17 downregulation and is effectively countered with N-Acetyl-L-cysteine (NAC) treatment in vitro. Therefore, reversing GSH-loss appears to be a promising treatment target for a translational approach. Since BALB/c mice have been described as sensitive in various models, we evaluated the toxic effect of biliatresone in robust C57BL/6J mice and confirmed its toxicity. Comparison between BALB/c and C57BL/6J mice revealed similarity in the toxic model, indicating that the onset of BA induced by biliatresone is independent of the strain specific different immune systems. Affected neonates exhibited clinical symptoms of BA, such as jaundice, ascites, clay-colored stools, yellow urine and impaired weight gain. The gallbladders of jaundiced neonates were hydropic and EHBD were twisted and enlarged. Serum and histological analysis proved cholestasis. No anomalies were seen in the liver and EHBD of control animals. With our study we join a chain of evidence confirming the BA-inducing effect of biliatresone.

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Section: Techniquesmentioning

confidence: 99%

Biliatresone and Biliary atresia – Disease modelling in C57BL/6J neonates

Schmidt

Hagens

Schuppert

et al. 2023

Preprint

Self Cite

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show abstract

“…The needle was slowly inserted in the umbilicus nearly parallel to the skin and directed to the upper right abdomen followed by slow injection and withdrawal. EHBD and liver were harvested using the En-bloc-Resection for murine neonatal EHBDs 72 . In brief, the access to the abdomen was ensured.…”

Section: Reagents and Toxinsmentioning

confidence: 99%

Biliatresone induces cholangiopathy in C57BL/6J neonates

Schmidt

Hagens

Schuppert

et al. 2023

Sci Rep

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Exposure to plant toxins or microbiota that are able to digest common food ingredients to toxic structures might be responsible for biliary atresia (BA). An isoflavonoid, biliatresone is known to effectively alter the extrahepatic bile duct (EHBD) development in BALB/c mice. Biliatresone causes a reduction of Glutathione (GSH) levels, SOX17 downregulation and is effectively countered with N-Acetyl-L-cysteine treatment in vitro. Therefore, reversing GSH-loss appears to be a promising treatment target for a translational approach. Since BALB/c mice have been described as sensitive in various models, we evaluated the toxic effect of biliatresone in robust C57BL/6J mice and confirmed its toxicity. Comparison between BALB/c and C57BL/6J mice revealed similarity in the toxic model. Affected neonates exhibited clinical symptoms of BA, such as jaundice, ascites, clay-colored stools, yellow urine and impaired weight gain. The gallbladders of jaundiced neonates were hydropic and EHBD were twisted and enlarged. Serum and histological analysis proved cholestasis. No anomalies were seen in the liver and EHBD of control animals. With our study we join a chain of evidence confirming that biliatresone is an effective agent for cross-lineage targeted alteration of the EHBD system.

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Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

Cited by 2 publications

References 0 publications

Biliatresone and Biliary atresia – Disease modelling in C57BL/6J neonates

Biliatresone and Biliary atresia – Disease modelling in C57BL/6J neonates

Biliatresone induces cholangiopathy in C57BL/6J neonates

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