Extraintestinal Pathogenic Escherichia coli

Russo, Thomas A.; Johnson, James R.

doi:10.1016/b978-0-12-369408-9.00048-2

Cited by 4 publications

(4 citation statements)

References 235 publications

(167 reference statements)

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“…The virulence score used to classify the ExPEC isolates was calculated using the total number of VFs genes. Isolates were classified as ExPEC if they were positive for two or more of the tested virulence genes [5]. The iut A (aerobactin acquisition), pap C and iha (adhesins), fyu A and irp 2 (iron capture systems), tra T (protectins) were the common detected genes, whereas usp (uropathogenic-specific proteins) and some of the adhesin genes ( hra , ibe A, and pap G1) were the least.…”

Section: Resultsmentioning

confidence: 99%

“…Gene amplification was done for bla TEM ; aminoglycoside resistant genes [ Ia (aphA1), Ia (aphA2)], integrase genes I with II; and 18 virulent factors for ExPEC adhesions, toxins, iron capture systems, protectins, uropathogenic specific protein and aerobactin system (Additional file 1: S3 file) as previously reported [11, 30, 31]. Virulence factors (Vfs) for all the E. coli isolates were tested using primers by Johnson et al [5, 6, 9, 11, 30] and other authors [31–39] as these were reported to be sufficient to identify ExPECs. The virulent genes were amplified in 6 primer pools [1 ( iron , sfa , iut A, hra ), 2 ( pap A, KpsM TIII, ire A, ibe A), 3 ( pap G1, pap GII, III, iha , omp T, KpsM TII), 4 ( iuC , Cnf 1, irp 2), 5 ( hly D, usp , tra T), and 6 ( pap C, sat , Fyu A)] [11, 30, 31].…”

Section: Methodsmentioning

confidence: 99%

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Escherichia coli bacteriuria in pregnant women in Ghana: antibiotic resistance patterns and virulence factors

et al. 2018

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ObjectivesThe relevance of Escherichia coli associated bacteriuria infection in pregnant women is poorly understood, despite these strains sharing a similar virulence profile with other pathogenic E. coli causing severe obstetric and neonatal infections. We characterized and determined the antimicrobial susceptibility, resistance genes and virulence profiles of 82 E. coli isolates associated with asymptomatic bacteriuria in some pregnant in Ghana from February to August 2016 using Kirby–Bauer disc diffusion and polymerase chain reaction.ResultsHigh levels of antimicrobial resistance were observed to ampicillin (79.3%), tetracycline (70.7%) and cotrimoxazole (59.8%), except for cefuroxime (32.9%). Resistance genes analyses revealed 58.5% were positive for BlaTEM and 7.3% for aph(3)-Ia(aphA2). Virulence factors (VFs) was more widespread in pregnant women in the 2nd and 3rd trimesters than 1st trimester. VFs relating to adhesion (papC and iha), Protectins (traT), aerobactin acquisition (iutA) and iron acquisition systems (fyuA and irp2) were more prevalent in the resistant E. coli isolates. This study provides evidence for a link in bacteriuria and transmission of extra-intestinal E. coli in pregnant women to cause multi-resistant obstetric or neonatal infections. Considering the involvement of extra-intestinal E. coli in infections, results are helpful to develop strategies to prevent maternal and/ neonatal infections.Electronic supplementary materialThe online version of this article (10.1186/s13104-018-3989-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Escherichia coli bacteriuria in pregnant women in Ghana: antibiotic resistance patterns and virulence factors

et al. 2018

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“…Escherichia coli is an important zoonotic bacterial pathogen ( Johnson and Russo, 2005 ; Russo and Johnson, 2009 ; Yin and Wan, 2019 ). More research is required to improve our understanding of this pathogen, including the analysis of its antibiotic resistance, virulence genes and genetic origin, to better protect vulnerable and endangered animals such as the giant panda.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance, serogroups, virulence gene profiles and MLST of Escherichia coli from giant panda

Li,

Lv,

et al. 2024

Front. Microbiol.

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Escherichia coli is a major bacterial pathogen which causes diarrhea in the giant panda. This study investigated the biological characteristics of 100 E. coli strains isolated from fecal samples collected from 100 captive giant pandas of different age groups and sexes. A standard Kirby-Bauer disk diffusion antimicrobial susceptibility test was performed with the isolates and we then further evaluated the antibiotic resistance genes (ARGs) by high-throughput quantitative PCR. Additionally, we then analyzed O serogroups through a slide agglutination test, virulence genes and the multi-locus sequence typing (MLST) by PCR. Antimicrobial susceptibility testing demonstrated that the 100 E. coli strains were mainly resistant to ENR (68%), AM (56%), IPM (55%), AMX (54%) and CA (52%), but were susceptible to MEM and FOX. The resistance to TZP, AK, FEP, CAZ, AMS, AZM, AT and IPM was significantly related to age (p < 0.05); the resistance rate of E. coli isolated from female giant pandas to N was significantly higher than in males (p < 0.05). Forty-five different types of ARGs were found, which included a total of 2,258 ARGs, in the 100 E. coli isolates. The top 10 of detection rate of ARGs were: acrA-04, acrA-05, aacC, blaCTX-M-04, ampC-04, blaSHV-01, blaTEM, sul2, blaOXY, tetA-02. ARGs aac (6’)I1, blaCTX-M-03, tetD-02, blaSHV-02 and blaOXY were significantly related to age (p < 0.05), blaSHV-02, blaNDM and ampC-04 were related to sex (p < 0.05). Twelve different O serogroups from 32 E. coli isolates were distinguished, including O4, O8, O9, O15, O18, O20, O55, O88, O112, O157, O158, and O167. The most prevalent O serotype was O20, but O28, O45, O101, O149, and O152 were not detected. Fourteen different types of virulence genes were detected in the 100 E. coli isolates, of which papA (99%) were highly detected, while hlyA, elt and estA were not detected. MLST showed that 41 STs, which had one CCs and six groups with SLVs, in the 100 E. coli strains were identified, the main type was ST37. Our results advocate the need of strict biosecurity and surveillance programs in order to prevent the spread of pathogenic bacteria in the captive giant panda population.

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“…In the last decades, the rod shaped gram-negative lactose fermenter and gas producing member of the family Enterobacteriaceae called Escherichia coli is reported to be a major cause of UTI in pregnancy [2, 3]. There are many pathotypes of E. coli , but the pathotype associated with extra intestinal infection is called extra intestinal pathogenic Escherichia coli (ExPEC) [4, 5, 6]. ExPEC are characterized by the presence of large numbers of specialized virulent factors (VFs) that enable them to become invasive, adhesive, and resistant to bactericidal drugs or resistant to phagocytosis in the host [7, 8].…”

Section: Introductionmentioning

confidence: 99%

Escherichia coliBcteriuria in pregnant women in Ghana: Antibiotic resistance pattern, Virulence Factors and Resistant genetic markers

Forson

Bright

Nana-Adjei

et al. 2018

Preprint

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45The relevance of Escherichia coli associated bacteriuria infection in pregnant women is poorly 46 understood, despite these strains sharing a similar virulence profile with other extra intestinal 47 pathogenic E. coli producing severe obstetric and neonatal infections. We characterized and 48 determined the antimicrobial susceptibility, resistant genes and virulence profiles of 82 E. coli 49 isolates associated with asymptomatic bacteriuria in some pregnant in five very distinct 50 hospitals in the Volta region from January, 2016 to April, 2016 using Kirby-Bauer disc 51 diffusion and polymerase chain reaction. 52High levels of antimicrobial resistance was observed to Ampicillin (79.3%), Tetracycline 53 (70.7%) and Cotrimoxazole (59.8%), except for Cefuroxime (32.9%). Resistant genes analyses 54 revealed 58.5% were positive for Bla TEM and 14.6% for aph(3)-Ia(aphA2). Virulence factors 55 (VFs) was more widespread in pregnant women in the 2 nd and 3 rd trimesters than 1 st trimester. 56VFs relating to adhesion (papC and iha), Protectins (traT), aerobactin acquisition (iutA) and 57 iron acquisition systems (fyuA and irp2) were more prevalent in the resistant E. coli isolates. 58This study provides additional evidence for a link in bacteriuria and transmission of extra-59 intestinal E. coli in pregnant women to cause multi-resistant severe obstetric or neonatal 60 infections. Considering the involvement of extra-intestinal E. coli in infections, our results may 61 be helpful to develop strategies to prevent maternal and/ neonatal infections. In addition 62 continuous surveillance is required to guide appropriate antibiotic usage in pregnant women. 64Maternal genitourinary infection is a leading cause of pregnancy complications worldwide [1]. 74 In the last decades, the rod shaped gram-negative lactose fermenter and gas producing member 75 of the family Enterobacteriaceae called Escherichia coli is reported to be a major cause of UTI 76 in pregnancy [2, 3]. There are many pathotypes of E. coli, but the pathotype associated with 77 extra intestinal infection is called extra intestinal pathogenic Escherichia coli (ExPEC) [4, 5, 78 6]. ExPEC are characterized by the presence of large numbers of specialized virulent factors 79(VFs) that enable them to become invasive, adhesive, and resistant to bactericidal drugs or 80 resistant to phagocytosis in the host [7, 8]. ExPEC lack the ability to cause gastroenteritis in 81 humans [7, 9], however, they are able to cause extra-intestinal infections involving sepsis, 82 meningitis, cellulitis, osteomyelitis, wounds infections and urinary tract [9, 10, 11]. 83ExPEC infections are common causes of healthcare-associated infection in recent years with a 84 large number reported to be associated with bacteriuria, bacteraemia and urosepsis [12][13][14][15]. 85Urinary tracts infections (UTI) is treatable, however, it is becoming increasingly difficult to 86 control because of rampant antimicrobial resistance to pregnancy friendly antibiotics, 87 especially those belonging to t...

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Extraintestinal Pathogenic Escherichia coli

Cited by 4 publications

References 235 publications

Escherichia coli bacteriuria in pregnant women in Ghana: antibiotic resistance patterns and virulence factors

Escherichia coli bacteriuria in pregnant women in Ghana: antibiotic resistance patterns and virulence factors

Antimicrobial resistance, serogroups, virulence gene profiles and MLST of Escherichia coli from giant panda

Escherichia coliBcteriuria in pregnant women in Ghana: Antibiotic resistance pattern, Virulence Factors and Resistant genetic markers

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