Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution

Mora, Azucena; López, Cecilia; Dabhi, Ghizlane; Blanco, Miguel; Blanco, Jesús E.; Alonso, Marı́a Pilar; Herrera, Alexandra; Mamani, Rosalía; Bonacorsi, Stéphane; Moulin-Schouleur, Maryvonne; Blanco, Jorge

doi:10.1186/1471-2180-9-132

Cited by 116 publications

(91 citation statements)

References 29 publications

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“…We preferred the "twohit" infection model over a single-infusion model because the hypotension observed with live E. coli challenge is attenuated by the prime, allowing more opportunity for acute lung injury resembling sepsis-induced acute respiratory distress syndrome (17,18). The E. coli O1:K1:H7 strain (American Type Culture Collection) was chosen given its activity as an extraintestinal pathogen and uropathogen (19,20) when administered intravenously, along with demonstrated survival and growth outside of an intestinal environment (21). Animals were observed post-challenge for the onset of clinical symptoms.…”

Section: Methods Summarymentioning

confidence: 99%

Integrative “Omic” Analysis of Experimental Bacteremia Identifies a Metabolic Signature That Distinguishes Human Sepsis from Systemic Inflammatory Response Syndromes

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Baron

et al. 2014

Am J Respir Crit Care Med

102

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Rationale: Sepsis is a leading cause of morbidity and mortality. Currently, early diagnosis and the progression of the disease are difficult to make. The integration of metabolomic and transcriptomic data in a primate model of sepsis may provide a novel molecular signature of clinical sepsis.Objectives: To develop a biomarker panel to characterize sepsis in primates and ascertain its relevance to early diagnosis and progression of human sepsis.Methods: Intravenous inoculation of Macaca fascicularis with Escherichia coli produced mild to severe sepsis, lung injury, and death. Plasma samples were obtained before and after 1, 3, and 5 days of E. coli challenge and at the time of killing. At necropsy, blood, lung, kidney, and spleen samples were collected. An integrative analysis of the metabolomic and transcriptomic datasets was performed to identify a panel of sepsis biomarkers. Measurements and Main Results:The extent of E. coli invasion, respiratory distress, lethargy, and mortality was dependent on the bacterial dose. Metabolomic and transcriptomic changes characterized severe infections and death, and indicated impaired mitochondrial, peroxisomal, and liver functions. Analysis of the pulmonary transcriptome and plasma metabolome suggested impaired fatty acid catabolism regulated by peroxisome-proliferator activated receptor signaling. A representative four-metabolite model effectively diagnosed sepsis in primates (area under the curve, 0.966) and in two human sepsis cohorts (area under the curve, 0.78 and 0.82).Conclusions: A model of sepsis based on reciprocal metabolomic and transcriptomic data was developed in primates and validated in two human patient cohorts. It is anticipated that the identified parameters will facilitate early diagnosis and management of sepsis.

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Section: Methods Summarymentioning

confidence: 99%

Integrative “Omic” Analysis of Experimental Bacteremia Identifies a Metabolic Signature That Distinguishes Human Sepsis from Systemic Inflammatory Response Syndromes

Tipper

Bruse

Baron

et al. 2014

Am J Respir Crit Care Med

102

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“…XbaI PFGE analysis was performed as previously described (21). Profiles were analyzed with the BioNumerics fingerprinting software (Applied Maths, St-Martens-Latem, Belgium).…”

Section: Methodsmentioning

confidence: 99%

“…The detection of 40 ExPEC-associated virulence genes was done by multiplex PCR (21,22 (Table 1). Isolates were classified as ExPEC if they carried Ն2 of papEF (P fimbriae), sfa/focDE (S/F1C fimbriae), afa/draBC (Afa/Dr adhesins), iutA (aerobactin receptor), and kpsM II (group 2 capsule synthesis) (23).…”

Section: Methodsmentioning

confidence: 99%

Four Main Virotypes among Extended-Spectrum-β-Lactamase-Producing Isolates of Escherichia coli O25b:H4-B2-ST131: Bacterial, Epidemiological, and Clinical Characteristics

et al. 2013

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“…APEC, however, mainly belongs to group B2 and to a lesser extent to group D (Mora et al, 2009). According to the ''mix and match'' hypothesis (Mokady et al, 2005), a successful clone of E. coli is likely to develop and spread horizontally and vertically.…”

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confidence: 99%

Multi-locus sequence typing and plasmid profile characterization of avian pathogenicEscherichia coliassociated with increased mortality in free-range layer flocks

et al. 2011

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Avian pathogenic Escherichia coli strains originating from 10 free-range layer flocks were characterized by multi-locus sequence typing and plasmid profile analysis to investigate their phylogenetic relationship and diversity, respectively. In addition to colibacillosis, all flocks tested positive for antibodies against avian metapneumovirus (aMPV) during production, and six of the flocks were concurrently affected by histomonosis. Accumulated average mortality for flocks concurrently affected by colibacillosis and histomonosis made up 17.4%, while the average mortality for E. coli-infected flocks was 16.5%. A total of eight different sequence types (STs) and 47 different plasmid profiles were demonstrated among the E. coli isolates. Within each flock between one and four different STs and between three and 13 different plasmid profiles were demonstrated. A statistical significant difference in STs and plasmid profile diversity of the population of E. coli was not demonstrated between flocks affected by histomonosis compared with histomonosis-free flocks. Only minor clonal diversity was demonstrated for each flock, and in all but one flock colibacillosis started before antibodies against aMPV were detected. All isolates, except two, carried plasmids greater than 100 kb, but only a single plasmid replicon type, IncFIB, was demonstrated, suggesting plasmids representing this type might represent a common pathogenicity factor for the different STs of E. coli. Within each flock a clonal tendency was observed, indicating that only certain clones of E. coli possess a significant pathogenic potential. These clones act as primary rather than secondary pathogens, resulting in colibacillosis without predisposing factors, including histomonosis and aMPV.

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Extraintestinal pathogenic Escherichia coli O1:K1:H7/NM from human and avian origin: detection of clonal groups B2 ST95 and D ST59 with different host distribution

Cited by 116 publications

References 29 publications

Integrative “Omic” Analysis of Experimental Bacteremia Identifies a Metabolic Signature That Distinguishes Human Sepsis from Systemic Inflammatory Response Syndromes

Integrative “Omic” Analysis of Experimental Bacteremia Identifies a Metabolic Signature That Distinguishes Human Sepsis from Systemic Inflammatory Response Syndromes

Four Main Virotypes among Extended-Spectrum-β-Lactamase-Producing Isolates of Escherichia coli O25b:H4-B2-ST131: Bacterial, Epidemiological, and Clinical Characteristics

Multi-locus sequence typing and plasmid profile characterization of avian pathogenicEscherichia coliassociated with increased mortality in free-range layer flocks

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